Dog Fibroblast Growth Factor 23 (FGF23) ELISA Kit | FGF23 elisa kit

Canine Fibroblast Growth Factor 23, FGF23 ELISA Kit

Cat. Num. AAA932371

• Gene Names: FGF23; ADHR; FGFN; HYPF; HPDR2; PHPTC
• Reactivity: Dog
• Synonyms: Fibroblast Growth Factor 23 (FGF23); Canine Fibroblast Growth Factor 23; FGF23 ELISA Kit; Canine Fibroblast Growth Factor 23 (FGF23) ELISA Kit; ADHR; HPDR2; HYPF; PHPTC; tumor-derived hypophosphatemia inducing factor; FGF23 elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of canine FGF23. No significant cross-reactivity or interference between canine FGF23 and analogues was observed.
• Sequence Length: 251

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 3.12 pg/ml-200 pg/ml
• Sensitivity: < 0.78 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FGF23 elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FGF23 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FGF23 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FGF23 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FGF23 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 10190674
• NCBI GeneID: 8074
• NCBI Accession #: NP_065689.1
• NCBI GenBank Nucleotide #: NM_020638.2
• UniProt Accession #: Q9GZV9; Q4V758
• Molecular Weight: 27,954 Da
• NCBI Official Full Name: fibroblast growth factor 23
• NCBI Official Synonym Full Names: fibroblast growth factor 23
• NCBI Official Symbol: FGF23
• NCBI Official Synonym Symbols: ADHR; FGFN; HYPF; HPDR2; PHPTC
• NCBI Protein Information: fibroblast growth factor 23; phosphatonin; tumor-derived hypophosphatemia inducing factor
• UniProt Protein Name: Fibroblast growth factor 23
• Protein Family: Fibroblast growth factor
• UniProt Gene Name: FGF23
• UniProt Synonym Gene Names: HYPF; UNQ3027/PRO9828; FGF-23
• UniProt Entry Name: FGF23_HUMAN

NCBI Description

This gene encodes a member of the fibroblast growth factor family of proteins, which possess broad mitogenic and cell survival activities and are involved in a variety of biological processes. The product of this gene regulates phosphate homeostasis and transport in the kidney. The full-length, functional protein may be deactivated via cleavage into N-terminal and C-terminal chains. Mutation of this cleavage site causes autosomal dominant hypophosphatemic rickets (ADHR). Mutations in this gene are also associated with hyperphosphatemic familial tumoral calcinosis (HFTC). [provided by RefSeq, Feb 2013]

Uniprot Description

FGF23: Regulator of phosphate homeostasis. Inhibits renal tubular phosphate transport by reducing SLC34A1 levels. Upregulates EGR1 expression in the presence of KL. Acts directly on the parathyroid to decrease PTH secretion. Regulator of vitamin-D metabolism. Negatively regulates osteoblast differentiation and matrix mineralization. Defects in FGF23 are the cause of autosomal dominant hypophosphataemic rickets (ADHR). ADHR is characterized by low serum phosphorus concentrations, rickets, osteomalacia, leg deformities, short stature, bone pain and dental abscesses. Defects in FGF23 are a cause of hyperphosphatemic familial tumoral calcinosis (HFTC). HFTC is a severe autosomal recessive metabolic disorder that manifests with hyperphosphatemia and massive calcium deposits in the skin and subcutaneous tissues. Belongs to the heparin-binding growth factors family.

Protein type: Secreted, signal peptide; Secreted; Cytokine

Chromosomal Location of Human Ortholog: 12p13.3

Cellular Component: extracellular space; extracellular region

Molecular Function: growth factor activity; type 1 fibroblast growth factor receptor binding

Biological Process: epidermal growth factor receptor signaling pathway; fibroblast growth factor receptor signaling pathway; phosphoinositide-mediated signaling; nerve growth factor receptor signaling pathway; positive regulation of transcription, DNA-dependent; negative regulation of hormone secretion; phosphate metabolic process; negative regulation of bone mineralization; cellular phosphate ion homeostasis; insulin receptor signaling pathway; innate immune response; negative regulation of osteoblast differentiation; phosphate ion homeostasis; cell differentiation; vitamin D catabolic process

Disease: Hypophosphatemic Rickets, Autosomal Dominant