Dog brain natriuretic peptide (BNP) ELISA Kit | BNP elisa kit

Canine brain natriuretic peptide, BNP ELISA Kit

Cat. Num. AAA922862

• Gene Names: NPPB; BNP
• Reactivity: Dog
• Synonyms: brain natriuretic peptide (BNP); Canine brain natriuretic peptide; BNP ELISA Kit; Canine brain natriuretic peptide (BNP) ELISA Kit; BNP; brain type natriuretic peptide; natriuretic protein; BNP elisa kit

• Reactivity: Dog
• Specificity: This assay has high sensitivity and excellent specificity for detection of canine BNP. No significant cross-reactivity or interference between canine BNP and analogues was observed.
• Sequence Length: 132

• Samples: Serum, plasma, tissue homogenates
• Assay Type: Quantitative Sandwich
• Detection Range: 12.5 pg/ml-800 pg/ml
• Sensitivity: <3.12 pg/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for BNP elisa kit

Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for BNP has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any BNP present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for BNP is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of BNP bound in the initial step. The color development is stopped and the intensity of the color is measured.

References

A new method of building permanent A-V block model: ablating his-bundle potential through femoral artery with pre-implanted biventricular pacemaker. Zheng Cheng, Ye Hai-ge, Li Jin, Ye Wan-chun, Wang Lu-ping, Li Yue-chun and Lin Jia-Feng (2014)

NCBI and Uniprot Product Information

• NCBI GI #: 57163785
• NCBI GeneID: 493764
• NCBI Accession #: NP_001009244.1
• NCBI GenBank Nucleotide #: NM_001009244.1
• UniProt Accession #: Q9GLK4
• Molecular Weight: 14,344 Da
• NCBI Official Full Name: natriuretic peptides B
• NCBI Official Symbol: NPPB
• NCBI Official Synonym Symbols: BNP
• NCBI Protein Information: natriuretic peptides B; brain natriuretic peptide; gamma-brain natriuretic peptide; natriuretic peptide precursor B
• UniProt Protein Name: Natriuretic peptides B
• UniProt Gene Name: NPPB
• UniProt Synonym Gene Names: BNP; BNP-32
• UniProt Entry Name: ANFB_FELCA

Uniprot Description

Function: Cardiac hormone which may function as a paracrine antifibrotic factor in the heart. Also plays a key role in cardiovascular homeostasis through natriuresis, diuresis, vasorelaxation, and inhibition of renin and aldosterone secretion. Specifically binds and stimulates the cGMP production of the NPR1 receptor. Binds the clearance receptor NPR3