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Western Blot (WB) (Western blot analysis of lysates from K562, mouse F9 cell line (from left to right), using Dnmt1 Antibody (Center). MBS9213109 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Rabbit anti-Human, mouse Dnmt1 Polyclonal Antibody | anti-Dnmt1 antibody

(Mouse) Dnmt1 Antibody (Center)

Gene Names
Dnmt1; Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
Reactivity
Human, mouse
Applications
Western Blot, ELISA
Purity
Purified Rabbit Polyclonal Antibody (Pab)
Synonyms
Dnmt1; Polyclonal Antibody; (Mouse) Dnmt1 Antibody (Center); DNA (cytosine-5)-methyltransferase 1; Met-1; DNA methyltransferase MmuI; DNA MTase MmuI; MMmuI; MCMT; Dnmt; Met1; Uim; anti-Dnmt1 antibody
Ordering
For Research Use Only!
Host
Rabbit
Reactivity
Human, mouse
Clonality
Polyclonal
Isotype
Rabbit Ig
Specificity
This MouseDnmt1 antibody is generated from a rabbit immunized with a KLH conjugated synthetic peptide between 881-914 amino acids from the Central region of mouse Dnmt1.
Purity/Purification
Purified Rabbit Polyclonal Antibody (Pab)
Form/Format
Purified polyclonal antibody supplied in PBS with 0.09% (W/V) sodium azide. This antibody is purified through a protein A column, followed by peptide affinity purification.
Concentration
Vial Concentration: 0.5 (varies by lot)
Sequence Length
1,502
Applicable Applications for anti-Dnmt1 antibody
Western Blot (WB), ELISA (EIA)
Application Notes
WB~~1:1000
Crown Antibody
Yes
Antigen Source
HUMAN
Preparation and Storage
Maintain refrigerated at 2-8 degree C for up to 6 months. For long term storage store at -20 degree C in small aliquots to prevent freeze-thaw cycles.

Western Blot (WB)

(Western blot analysis of lysates from K562, mouse F9 cell line (from left to right), using Dnmt1 Antibody (Center). MBS9213109 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB) (Western blot analysis of lysates from K562, mouse F9 cell line (from left to right), using Dnmt1 Antibody (Center). MBS9213109 was diluted at 1:1000 at each lane. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysates at 20ug per lane.)

Western Blot (WB)

(Western blot analysis of lysate from K562 cell line, using Dnmt1 Antibody (Center). MBS9213109 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)

Western Blot (WB) (Western blot analysis of lysate from K562 cell line, using Dnmt1 Antibody (Center). MBS9213109 was diluted at 1:1000. A goat anti-rabbit IgG H&L(HRP) at 1:10000 dilution was used as the secondary antibody. Lysate at 20ug.)
Related Product Information for anti-Dnmt1 antibody
Methylates CpG residues. Preferentially methylates hemimethylated DNA. Associates with DNA replication sites in S phase maintaining the methylation pattern in the newly synthesized strand, that is essential for epigenetic inheritance. Associates with chromatin during G2 and M phases to maintain DNA methylation independently of replication. It is responsible for maintaining methylation patterns established in development. DNA methylation is coordinated with methylation of histones. Mediates transcriptional repression by direct binding to HDAC2. In association with DNMT3B and via the recruitment of CTCFL/BORIS, involved in activation of BAG1 gene expression by modulating dimethylation of promoter histone H3 at H3K4 and H3K9.
Product Categories/Family for anti-Dnmt1 antibody
References
Bestor T.H.,et al.J. Mol. Biol. 203:971-983(1988).
Yoder J.A.,et al.J. Biol. Chem. 271:31092-31097(1996).
Aguirre-Arteta A.M.,et al.Cell Growth Differ. 11:551-559(2000).
Margot J.B.,et al.J. Mol. Biol. 297:293-300(2000).
Mertineit C.,et al.Development 125:889-897(1998).

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
183189
NCBI Official Full Name
DNA (cytosine-5)-methyltransferase 1 isoform 1
NCBI Official Synonym Full Names
DNA methyltransferase (cytosine-5) 1
NCBI Official Symbol
Dnmt1
NCBI Official Synonym Symbols
Dnmt; MCMT; Met1; Cxxc9; MTase; Met-1; Dnmt1o; m.MmuI; MommeD2
NCBI Protein Information
DNA (cytosine-5)-methyltransferase 1
UniProt Protein Name
DNA (cytosine-5)-methyltransferase 1
UniProt Gene Name
Dnmt1
UniProt Synonym Gene Names
Dnmt; Met1; Uim; Dnmt1; Met-1; DNA MTase MmuI; M.MmuI
UniProt Entry Name
DNMT1_MOUSE

Uniprot Description

DNMT1: an ubiquitous DNA methyltransferase that methylates CpG residues. Preferentially methylates hemimethylated DNA. It is responsible for maintaining methylation patterns established in development, and may play an active role in DNA damage repair. Mediates transcriptional repression by direct binding to HDAC2. Its abundance is reduced to non detectable levels at the G0 phase of the cell cycle and is dramatically induced upon entrance into the S-phase of the cell cycle. Interacts with HDAC1 and with PCNA. Forms a complex with DMAP1 and HDAC2, with direct interaction. Forms also a stable complex with E2F1, BB1 and HDAC1. Binds MBD2 and MBD3. Three isoforms of the human protein produced by alternative splicing have been described.

Protein type: Methyltransferase, DNA; Cell development/differentiation; Transcription regulation; EC 2.1.1.37; Methyltransferase; Amino Acid Metabolism - cysteine and methionine

Cellular Component: heterochromatin; centric heterochromatin; protein complex; cell soma; cytoplasm; replication fork; nucleus

Molecular Function: protein domain specific binding; methyltransferase activity; methyl-CpG binding; zinc ion binding; unmethylated CpG binding; RNA binding; histone deacetylase binding; metal ion binding; DNA-methyltransferase activity; transcription factor binding; transferase activity; DNA (cytosine-5-)-methyltransferase activity; protein binding; DNA binding; DNA (cytosine-5-)-methyltransferase activity, acting on CpG substrates; double-stranded DNA binding; estrogen receptor binding; chromatin binding; catalytic activity

Biological Process: negative regulation of histone H3-K9 methylation; methylation; maintenance of DNA methylation; transcription, DNA-dependent; metabolic process; S-adenosylmethionine metabolic process; S-adenosylhomocysteine metabolic process; gene silencing; negative regulation of transcription from RNA polymerase II promoter; positive regulation of histone H3-K4 methylation; chromatin modification; regulation of cell proliferation; cytosine methylation within a CG sequence; regulation of transcription, DNA-dependent; regulation of gene expression; DNA methylation; S-adenosylmethioninamine metabolic process; negative regulation of transcription, DNA-dependent; DNA methylation on cytosine

Research Articles on Dnmt1

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Product Notes

The Dnmt1 dnmt1 (Catalog #AAA9213109) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The (Mouse) Dnmt1 Antibody (Center) reacts with Human, mouse and may cross-react with other species as described in the data sheet. AAA Biotech's Dnmt1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), ELISA (EIA). WB~~1:1000. Researchers should empirically determine the suitability of the Dnmt1 dnmt1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Dnmt1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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