DNA polymerase III subunit beta (dnaN) Recombinant Protein | dnaN recombinant protein
Recombinant Caulobacter crescentus DNA polymerase III subunit beta (dnaN)
Purity
Greater or equal to 85% purity as determined by SDS-PAGE.
Synonyms
DNA polymerase III subunit beta (dnaN); Recombinant Caulobacter crescentus DNA polymerase III subunit beta (dnaN); dnaN recombinant protein
Host
E Coli or Yeast or Baculovirus or Mammalian Cell
Purity/Purification
Greater or equal to 85% purity as determined by SDS-PAGE.
Form/Format
Lyophilized or liquid (Format to be determined during the manufacturing process)
Sequence Positions
1-372, Full length protein
Sequence
MKLTIERAALLKALGHVQSVVERRNTIPILSNILLSAEGDRLSFSATDLDMEIIDEGFAQIDVPGQITAPAHTLYEIVRKLPDGADVSLSFSGDDPRLVIQAGRSRFNLPVLPAGDFPVMSSDGLSSRIAVDTNELIRLIDKTRFAISTEETRYYLNGLYVHTVNEGGETKLRAVATDGHRLALAEMPAPEGAVGIPGVIVPRKTIAEARRLMESAGETVDLQVSPQKVRFEFGAAALTSKVIDGAFPDYMRVIPRDNAKILTLDNDLFAKAVDRVATISAEKSRSVKLAVEPGRITLTVRNMEAGQAVEEVEVDYDGEPFEIGFNARYLLDVCGQIAGPQAEFRFADPASPTLVVDPVDPGVKYVLMPLRV
Sequence Length
372
Species
Caulobacter crescentus (strain NA1000 / CB15N)
Preparation and Storage
Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.
NCBI and Uniprot Product Information
NCBI GI #
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
40,425 Da
NCBI Official Full Name
DNA polymerase III subunit beta
UniProt Protein Name
Beta sliding clamp
Protein Family
UniProt Gene Name
dnaN
UniProt Synonym Gene Names
Beta clamp; Sliding clamp
Uniprot Description
Confers DNA tethering and processivity to DNA polymerases and other proteins. Acts as a clamp, forming a ring around DNA (a reaction catalyzed by the clamp-loading complex) which diffuses in an ATP-independent manner freely and bidirectionally along dsDNA. Initially characterized for its ability to contact the catalytic subunit of DNA polymerase III (Pol III), a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria; Pol III exhibits 3'-5' exonuclease proofreading activity. The beta chain is required for initiation of replication as well as for processivity of DNA replication (). Interaction with toxin SocB via the hydrophobic cleft blocks DNA replication elongation (PubMed:24239291).
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Product Notes
The dnaN dnan (Catalog #AAA1108238) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-372, Full length protein. The amino acid sequence is listed below: MKLTIERAAL LKALGHVQSV VERRNTIPIL SNILLSAEGD RLSFSATDLD MEIIDEGFAQ IDVPGQITAP AHTLYEIVRK LPDGADVSLS FSGDDPRLVI QAGRSRFNLP VLPAGDFPVM SSDGLSSRIA VDTNELIRLI DKTRFAISTE ETRYYLNGLY VHTVNEGGET KLRAVATDGH RLALAEMPAP EGAVGIPGVI VPRKTIAEAR RLMESAGETV DLQVSPQKVR FEFGAAALTS KVIDGAFPDY MRVIPRDNAK ILTLDNDLFA KAVDRVATIS AEKSRSVKLA VEPGRITLTV RNMEAGQAVE EVEVDYDGEP FEIGFNARYL LDVCGQIAGP QAEFRFADPA SPTLVVDPVD PGVKYVLMPL RV. It is sometimes possible for the material contained within the vial of "DNA polymerase III subunit beta (dnaN), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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