Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22329c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22329c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Rabbit anti-Human COL14A1 Polyclonal Antibody | anti-COL14A1 antibody
COL14A1 Antibody (Center)
FC: 1:25
WB: 1:2000
For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22329c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22329c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Flow Cytometry (FC/FACS)
(Overlay histogram showing Hela cells stained with AP22329c(green line). The cells were fixed with 2% paraformaldehyde (10 min) and then permeabilized with 90% methanol for 10 min. The cells were then icubated in 2% bovine serum albumin to block non-specific protein-protein interactions followed by the antibody (AP22329c, 1:25 dilution) for 60 min at 37 degree C. The secondary antibody used was Goat-Anti-Rabbit IgG, DyLight 488 Conjugated Highly Cross-Adsorbed(OE188374) at 1/200 dilution for 40 min at 37 degree C. Isotype control antibody (blue line) was rabbit IgG1 (1mug/1x10^6 cells) used under the same conditions. Acquisition of >10, 000 events was performed.)
Western Blot (WB)
(Anti-COL14A1 Antibody (Center) at 1:2000 dilution + Human lung lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Western Blot (WB)
(Anti-COL14A1 Antibody (Center) at 1:2000 dilution + Human lung lysateLysates/proteins at 20 ug per lane. SecondaryGoat Anti-Rabbit IgG, (H+L), Peroxidase conjugated at 1/10000 dilution. Predicted band size : 194 kDaBlocking/Dilution buffer: 5% NFDM/TBST.)
Immunohistochemistry
(Staining COL14A1 in human hearttissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.)
Immunohistochemistry
(Staining COL14A1 in human hearttissue sections by Immunohistochemistry(IHC-P - paraformaldehyde-fixed,paraffin-embedded sections). Tissue was fixedwith formaldehyde and blocked with 3% BSAfor 0. 5 hour at room temperature; antigenretrieval was by heat mediation with a citratebuffer (pH6). Samples were incubated withprimary antibody (1/25) for 1 hours at 37°C. A undiluted biotinylated goat polyvalent antibodywas used as the secondary antibody.)
NCBI and Uniprot Product Information
NCBI Description
This gene encodes the alpha chain of type XIV collagen, a member of the FACIT (fibril-associated collagens with interrupted triple helices) collagen family. Type XIV collagen interacts with the fibril surface and is involved in the regulation of fibrillogenesis. [provided by RefSeq, Jan 2013]
Uniprot Description
Plays an adhesive role by integrating collagen bundles. It is probably associated with the surface of interstitial collagen fibrils via COL1. The COL2 domain may then serve as a rigid arm which sticks out from the fibril and protrudes the large N-terminal globular domain into the extracellular space, where it might interact with other matrix molecules or cell surface receptors ().
Research Articles on COL14A1
Similar Products
Product Notes
The COL14A1 col14a1 (Catalog #AAA9231274) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The COL14A1 Antibody (Center) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's COL14A1 can be used in a range of immunoassay formats including, but not limited to, Immunohistochemistry (IHC) Paraffin, Flow Cytometry (FC/FACS), Western Blot (WB), ELISA (EIA). IHC-P: 1:25 FC: 1:25 WB: 1:2000. Researchers should empirically determine the suitability of the COL14A1 col14a1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "COL14A1, Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
