Rat Bcan (Brevican core protein) ELISA Kit | Bcan elisa kit

Rat Bcan (Brevican core protein) ELISA Kit

Cat. Num. AAA7608260

• Gene Names: Bcan; BEHAB; ALPBRE
• Reactivity: Rat
• Synonyms: Bcan (Brevican core protein); Rat Bcan (Brevican core protein) ELISA Kit; BCAN; BEHAB; Brevican; Brevican core protein; CSPG7; Bcan elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Bcan. No significant cross-reactivity or interference between Bcan and analogues was observed.

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 2-8 degree C for est. ~6 months

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for Bcan elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 75992948
• NCBI GeneID: 25393
• NCBI Accession #: NP_001028837.1
• NCBI GenBank Nucleotide #: NM_001033665.1
• UniProt Accession #: P55068; Q62860; Q63040; Q63513
• Molecular Weight: 69,085 Da
• NCBI Official Full Name: brevican core protein isoform 1
• NCBI Official Synonym Full Names: brevican
• NCBI Official Symbol: Bcan
• NCBI Official Synonym Symbols: BEHAB; ALPBRE
• NCBI Protein Information: brevican core protein; brain-enriched hyaluronan-binding protein; brevican (brain specific proteoglycan in the aggrecan family)
• UniProt Protein Name: Brevican core protein
• Protein Family: Brevican core protein
• UniProt Gene Name: Bcan
• UniProt Synonym Gene Names: Behab; BEHAB
• UniProt Entry Name: PGCB_RAT

NCBI Description

brain-specific member of the aggrecan family of chondroitin sulfate proteoglycans; extracellular matrix (ECM) protein; binds hyaluronan, a ubiquitous component of the ECM [RGD, Feb 2006]

Uniprot Description

BCAN: May play a role in the terminally differentiating and the adult nervous system during postnatal development. Could stabilize interactions between hyaluronan (HA) and brain proteoglycans. Belongs to the aggrecan/versican proteoglycan family. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted; Membrane protein, GPI anchor; Secreted, signal peptide

Cellular Component: extracellular space; proteinaceous extracellular matrix; dendrite; synapse

Molecular Function: extracellular matrix structural constituent; hyaluronic acid binding; carbohydrate binding

Biological Process: central nervous system development; hippocampus development; cell adhesion; skeletal development; regulation of neuronal synaptic plasticity