DnaJ Recombinant Protein | DNAJB2 recombinant protein

Recombinant Human DnaJ (Hsp40) Homolog, Subfamily B, Member 2

Cat. Num. AAA144248

• Gene Names: DNAJB2; HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• Purity: Greater than 90.0% as determined by SDS-PAGE.
• Synonyms: DnaJ; Recombinant Human DnaJ (Hsp40) Homolog; Subfamily B; Member 2; DNAJB2 Human; DnaJ (Hsp40) homolog; subfamily B; member 2 Human Recombinant; DnaJ homolog subfamily B member 2; DnaJ protein homolog 1; Heat shock 40 kDa protein 3; Heat shock protein J1; HSJ-1; DNAJB2; HSJ1; HSPF3; DNAJB2 recombinant protein

• Host: E Coli
• Purity/Purification: Greater than 90.0% as determined by SDS-PAGE.
• Form/Format: The DNAJB2 solution (1 mg/ml) contains 20mM Tris-HCl buffer(pH 8.0), 10% glycerol, 2mM DTT and 0.1M NaCl.; Sterile Filtered colorless solution.
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASYYEI LDVPRSASAD DIKKAYRRKA LQWHPDKNPD NKEFAEKKFK EVAEAYEVLS DKHKREIYDR YGREGLTGTG TGPSRAEAGS GGPGFTFTFR SPEEVFREFF GSGDPFAELF DDLGPFSELQ NRGSRHSGPF FTFSSSFPGH SDFSSSSFSF SPGAGAFRSV STSTTFVQGR RITTRRIMEN GQERVEVEED GQLKSVTING VPDDLALGLE LSRREQQPSV TSRSGGTQVQ QTPASCPLDS DLSEDEDLQL AMAYSLSEME AAGKKPADVF
• Sequence Length: 277

• Preparation and Storage: DNAJB2 should be stored desiccated below -18 degree C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA). Please prevent freeze-thaw cycles.

Related Product Information for DNAJB2 recombinant protein

Description: DNAJB2 Human Recombinant fused with a 23 amino acid His tag at N-terminus produced in E Coli is a single, non-glycosylated, polypeptide chain containing 300 amino acids (1-277 a.a.) and having a molecular mass of 33kDa. The DNAJB2 is purified by proprietary chromatographic techniques.

Introduction: DnaJB2 is a member of the DnaJ family. The DnaJ family is one of the largest of all the chaperone families which has evolved with diverse cellular localization and functions. DnaJB2 are important mediators of proteolysis which are involved in the regulation of protein degradation, exocytosis and endocytosis. The DnaJ proteins play a significant role in the HSP70 chaperone machine by interacting with HSP70 to stimulate ATP hydrolysis. DnaJB2 is expressed almost solely in the brain, with the highest levels in the frontal cortex and hippocampus.

Product Categories/Family for DNAJB2 recombinant protein

Heat Shock Proteins; Recombinant Proteins; Heat Shock Protein

NCBI and Uniprot Product Information

• NCBI GI #: 88501736
• NCBI GeneID: 3300
• NCBI Accession #: NP_001034639.1
• NCBI GenBank Nucleotide #: NM_001039550.1
• UniProt Accession #: P25686; Q8IUK1; Q8IUK2; Q96F52; A8K9P6
• Molecular Weight: 30,569 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 2 isoform a
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily B, member 2
• NCBI Official Symbol: DNAJB2
• NCBI Official Synonym Symbols: HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• NCBI Protein Information: dnaJ homolog subfamily B member 2; dnaJ protein homolog 1; heat shock 40 kDa protein 3; heat shock protein J1; heat shock protein, neuronal DNAJ-like 1
• UniProt Protein Name: DnaJ homolog subfamily B member 2
• Protein Family: DnaJ
• UniProt Gene Name: DNAJB2
• UniProt Synonym Gene Names: HSJ1; HSPF3; HSJ-1
• UniProt Entry Name: DNJB2_HUMAN

NCBI Description

This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

DNAJB2: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: proteasome complex; inclusion body; nucleus; cytosol

Molecular Function: protein binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: negative regulation of cell proliferation; ER-associated protein catabolic process; protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; negative regulation of cell growth; response to unfolded protein

Disease: Spinal Muscular Atrophy, Distal, Autosomal Recessive, 5; Charcot-marie-tooth Disease, Axonal, Type 2t