Rabbit anti-Human DNAJB2 Polyclonal Antibody | anti-DNAJB2 antibody

DNAJB2 (DnaJ Homolog Subfamily B Member 2, DnaJ Protein Homolog 1 Heat Shock 40kD Protein 3 Heat Shock Protein J1, HSJ-1, HSJ1, HSPF3) (HRP)

Cat. Num. AAA6376418

• Gene Names: DNAJB2; HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: DNAJB2; Polyclonal Antibody; DNAJB2 (DnaJ Homolog Subfamily B Member 2; DnaJ Protein Homolog 1 Heat Shock 40kD Protein 3 Heat Shock Protein J1; HSJ-1; HSJ1; HSPF3) (HRP); anti-DNAJB2 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human DNAJB2.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Horseradish Peroxidase (HRP).

• Applicable Applications for anti-DNAJB2 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human DNAJB2, aa1-277 (NP_001034639.1).
• Immunogen Sequence: MASYYEILDVPRSASADDIKKAYRRKALQWHPDKNPDNKEFAEKKFKEVAEAYEVLSDKHKREIYDRYGREGLTGTGTGPSRAEAGSGGPGFTFTFRSPEEVFREFFGSGDPFAELFDDLGPFSELQNRGSRHSGPFFTFSSSFPGHSDFSSSSFSFSPGAGAFRSVSTSTTFVQGRRITTRRIMENGQERVEVEEDGQLKSVTINGVPDDLALGLELSRREQQPSVTSRSGGTQVQQTPASCPLDSDLSEDEDLQLAMAYSLSEMEAAGKKPADVF
• Conjugate: HRP
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot analysis of DNAJB2 expression in transfected 293T cell line by DNAJB2 polyclonal antibody. Lane 1: DNAJB2 transfected lysate (30.6kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-DNAJB2 antibody

DnaJB2, also known as HSJ1 or HSPF3, is belongs to the DnaJ family. It is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The DnaJ proteins play a critical role in the HSP70 chaperone machine by interacting with HSP70 to stimulate ATP hydrolysis. DnaJB2 is important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis.

Product Categories/Family for anti-DNAJB2 antibody

Antibodies; Heat Shock Proteins

NCBI and Uniprot Product Information

• NCBI GI #: 88501736
• NCBI GeneID: 3300
• NCBI Accession #: NP_001034639.1
• NCBI GenBank Nucleotide #: NM_001039550.1
• UniProt Accession #: P25686; Q8IUK1; Q8IUK2; Q96F52; A8K9P6
• Molecular Weight: 30,569 Da
• NCBI Official Full Name: dnaJ homolog subfamily B member 2 isoform a
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily B, member 2
• NCBI Official Symbol: DNAJB2
• NCBI Official Synonym Symbols: HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• NCBI Protein Information: dnaJ homolog subfamily B member 2; dnaJ protein homolog 1; heat shock 40 kDa protein 3; heat shock protein J1; heat shock protein, neuronal DNAJ-like 1
• UniProt Protein Name: DnaJ homolog subfamily B member 2
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJB2
• UniProt Synonym Gene Names: HSJ1; HSPF3; HSJ-1
• UniProt Entry Name: DNJB2_HUMAN

NCBI Description

This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

DNAJB2: 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Chaperone

Chromosomal Location of Human Ortholog: 2q35

Cellular Component: proteasome complex; inclusion body; nucleus; cytosol

Molecular Function: protein binding; chaperone binding; unfolded protein binding; Hsp70 protein binding; polyubiquitin binding

Biological Process: negative regulation of cell proliferation; ER-associated protein catabolic process; protein folding; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein refolding; negative regulation of cell growth; response to unfolded protein

Disease: Spinal Muscular Atrophy, Distal, Autosomal Recessive, 5; Charcot-marie-tooth Disease, Axonal, Type 2t