DNAJB2 recombinant protein

DNAJB2, 1-277aa, Human, His tag, E Coli

Cat. Num. AAA203831

• Gene Names: DNAJB2; HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: DNAJB2; 1-277aa; Human; His tag; E Coli; DnaJ homolog subfamily B member 2; HSJ1; HSPF3; DNAJB2 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 10% glycerol, 2mM DTT, 0.1M NaCl
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMASYYEI LDVPRSASAD DIKKAYRRKA LQWHPDKNPD NKEFAEKKFK EVAEAYEVLS DKHKREIYDR YGREGLTGTG TGPSRAEAGS GGPGFTFTFR SPEEVFREFF GSGDPFAELF DDLGPFSELQ NRGSRHSGPF FTFSSSFPGH SDFSSSSFSF SPGAGAFRSV STSTTFVQGR RITTRRIMEN GQERVEVEED GQLKSVTING VPDDLALGLE LSRREQQPSV TSRSGGTQVQ QTPASCPLDS DLSEDEDLQL AMAYSLSEME AAGKKPADVF
• Sequence Length: 277

• Applicable Applications for DNAJB2 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for DNAJB2 recombinant protein

DnaJB2, also known as HSJ1 or HSPF3, is belongs to the DnaJ family. It is expressed almost exclusively in the brain, with the highest levels in the frontal cortex and hippocampus. The DnaJ family is one of the largest of all the chaperone families and has evolved with diverse cellular localization and functions. The DnaJ proteins play a critical role in the HSP70 chaperone machine by interacting with HSP70 to stimulate ATP hydrolysis. DnaJB2 is important mediators of proteolysis and are involved in the regulation of protein degradation, exocytosis and endocytosis. Recombinant human DNAJB2 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.

Product Categories/Family for DNAJB2 recombinant protein

Heat Shock Proteins

References

Stewart GR., et al. (2004) Tuberculosis. 84 (3-4):180-7. ; Tomoyasu T., et al. (1998) Mol Microbiol. 30 (3):567-81.;

NCBI and Uniprot Product Information

• NCBI GI #: 88501736
• NCBI GeneID: 3300
• NCBI Accession #: NP_001034639
• NCBI GenBank Nucleotide #: NM_001039550.1
• UniProt Accession #: P25686; Q53QD7; Q8IUK1; Q8IUK2; Q96F52; A8K9P6
• Molecular Weight: 33 kDa (300aa) confirmed by MALDI-TOF (Molecular weight on SDS-PAGE will appear higher)
• NCBI Official Full Name: dnaJ homolog subfamily B member 2 isoform a
• NCBI Official Synonym Full Names: DnaJ heat shock protein family (Hsp40) member B2
• NCBI Official Symbol: DNAJB2
• NCBI Official Synonym Symbols: HSJ1; CMT2T; DSMA5; HSJ-1; HSPF3
• NCBI Protein Information: dnaJ homolog subfamily B member 2
• UniProt Protein Name: DnaJ homolog subfamily B member 2
• Protein Family: DnaJ homolog subfamily
• UniProt Gene Name: DNAJB2
• UniProt Synonym Gene Names: HSJ-1

NCBI Description

This gene is almost exclusively expressed in the brain, mainly in the neuronal layers. It encodes a protein that shows sequence similarity to bacterial DnaJ protein and the yeast homologs. In bacteria, this protein is implicated in protein folding and protein complex dissociation. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2011]

Uniprot Description

Functions as a co-chaperone, regulating the substrate binding and activating the ATPase activity of chaperones of the HSP70/heat shock protein 70 family (PubMed:7957263, PubMed:22219199). In parallel, also contributes to the ubiquitin-dependent proteasomal degradation of misfolded proteins (PubMed:15936278, PubMed:21625540). Thereby, may regulate the aggregation and promote the functional recovery of misfolded proteins like HTT, MC4R, PRKN, RHO and SOD1 and be crucial for many biological processes (PubMed:12754272, PubMed:20889486, PubMed:21719532, PubMed:22396390, PubMed:24023695). Isoform 1 which is localized to the endoplasmic reticulum membranes may specifically function in ER-associated protein degradation of misfolded proteins (PubMed:15936278).