Human fetuin B ELISA Kit | FETUB elisa kit

Human Fetuin-B, FETUB ELISA Kit

Cat. Num. AAA935545

• Gene Names: FETUB; 16G2; Gugu; IRL685
• Reactivity: Human
• Synonyms: fetuin B; Human Fetuin-B; FETUB ELISA Kit; Human Fetuin-B (FETUB) ELISA kit; 16G2; Gugu; IRL685; OTTHUMP00000210121; fetuin-like protein; FETUB elisa kit

• Reactivity: Human
• Specificity: This assay has high sensitivity and excellent specificity for detection of human FETUB. No significant cross-reactivity or interference between human FETUB and analogues was observed.
• Sequence Length: 382

• Samples: Serum, plasma, tissue homogenates.
• Assay Type: Sandwich
• Detection Range: 0.156 ng/ml-10 ng/ml.
• Sensitivity: 0.039 ng/ml.
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
• Inter-assay Precision: Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for FETUB elisa kit

Principle of the Assay||This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for FETUB has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any FETUB present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for FETUB is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of FETUB bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

• NCBI GI #: 58331240
• NCBI GeneID: 26998
• NCBI Accession #: NP_055190.2
• NCBI GenBank Nucleotide #: NM_014375.2
• UniProt Accession #: Q9UGM5; Q1RMZ0; Q5J876; Q6DK58; Q6GRB6; Q9Y6Z0; B2RCW6; E9PG06
• Molecular Weight: 42,055 Da
• NCBI Official Full Name: fetuin-B
• NCBI Official Synonym Full Names: fetuin B
• NCBI Official Symbol: FETUB
• NCBI Official Synonym Symbols: 16G2; Gugu; IRL685
• NCBI Protein Information: fetuin-B; fetuin-like protein IRL685
• UniProt Protein Name: Fetuin-B
• Protein Family: Fetuin
• UniProt Gene Name: FETUB
• UniProt Entry Name: FETUB_HUMAN

NCBI Description

The protein encoded by this gene is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq, Jul 2008]

Uniprot Description

FETUB: is a member of the fetuin family, part of the cystatin superfamily of cysteine protease inhibitors. Fetuins have been implicated in several diverse functions, including osteogenesis and bone resorption, regulation of the insulin and hepatocyte growth factor receptors, and response to systemic inflammation. This protein may be secreted by cells. [provided by RefSeq, Jul 2008]

Protein type: Secreted, signal peptide; Secreted

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: extracellular space

Molecular Function: metalloendopeptidase inhibitor activity; cysteine protease inhibitor activity

Biological Process: binding of sperm to zona pellucida; single fertilization