Human HSP-40 (Heat Shock Protein 40) CLIA Kit | HSP-40 clia kit

Human HSP-40 (Heat Shock Protein 40) CLIA Kit

Cat. Num. AAA2532200

• Gene Names: DNAJB1; Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• Reactivity: Human
• Synonyms: HSP-40 (Heat Shock Protein 40); Human HSP-40 (Heat Shock Protein 40) CLIA Kit; HSP-40 clia kit

• Reactivity: Human
• Specificity: This kit recognizes natural and recombinant HSP-40. No significant cross-reactivity or interference between HSP-40 and analogues was observed.
• Sequence Length: 340

• Assay Type: Sandwich
• Detection Range: 31.25-2000pg/mL
• Sensitivity: Min: 18.75pg/mL; Max: 2000pg/mL

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for HSP-40 clia kit

Intended Uses: This CLIA kit applies to the in vitro quantitative determination of HSP-40 concentrations in serum, plasma and other biological fluids.

Principle of the Assay: This kit uses Sandwich-CLIA as the method. The micro CLIA plate provided in this kit has been pre-coated with an antibody specific to HSP-40. Standards or samples are added to the appropriate micro CLIA plate wells and combined with the specific antibody. Then a biotinylated detection antibody specific for HSP-40 and Avidin-Horseradish Peroxidase (HRP) conjugate is added to each micro plate well successively and incubated. Free components are washed away. The substrate solution is added to each well. Only those wells that contain HSP-40, biotinylated detection antibody and Avidin-HRP conjugate will appear fluorescence. The Relative light unit (RLU) value is measured spectrophotometrically by the Chemiluminescence immunoassay analyzer. The RLU value is positively associated with the concentration of HSP-40. You can calculate the concentration of HSP-40 in the samples by comparing the RLU value of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 1816452
• NCBI GeneID: 3337
• Molecular Weight: 27,016 Da
• NCBI Official Full Name: heat shock protein 40
• NCBI Official Synonym Full Names: DnaJ (Hsp40) homolog, subfamily B, member 1
• NCBI Official Symbol: DNAJB1
• NCBI Official Synonym Symbols: Hdj1; Sis1; HSPF1; Hsp40; RSPH16B
• NCBI Protein Information: dnaJ homolog subfamily B member 1
• UniProt Protein Name: DnaJ homolog subfamily B member 1
• UniProt Gene Name: DNAJB1
• UniProt Synonym Gene Names: DNAJ1; HDJ1; HSPF1; HSP40; Heat shock protein 40; hDj-1
• UniProt Entry Name: DNJB1_HUMAN

NCBI Description

This gene encodes a member of the DnaJ or Hsp40 (heat shock protein 40 kD) family of proteins. DNAJ family members are characterized by a highly conserved amino acid stretch called the 'J-domain' and function as one of the two major classes of molecular chaperones involved in a wide range of cellular events, such as protein folding and oligomeric protein complex assembly. The encoded protein is a molecular chaperone that stimulates the ATPase activity of Hsp70 heat-shock proteins in order to promote protein folding and prevent misfolded protein aggregation. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Sep 2015]

Uniprot Description

Interacts with HSP70 and can stimulate its ATPase activity. Stimulates the association between HSC70 and HIP.