DNA Polymerase I Klenow Fragment, Exo-Minus Recombinant Protein
DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant
Purity
Molecular Biology Grade
Synonyms
DNA Polymerase I Klenow Fragment; Exo-Minus; Recombinant; Exo-Minus recombinant protein
Host
E Coli
Purity/Purification
Molecular Biology Grade
Form/Format
Supplied as a liquid in 25mM Tris-HCl, pH 7.4, 0.1mM EDTA, 1mM DTT, 50% glycerol.
Application Notes
Suitable for use in labeling of DNA by the random primer method; labeling recessed 3'-termini of double-stranded DNA; DNA sequencing by the method of Sanger, F., et al.; Strand Displacement Amplification (SDA).
Quality Control
Endodeoxyribonuclease Assay:
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 20u of Klenow Fragment, exo-. with 1ug of pBR322 DNA in 50ul of reaction buffer for 4 hours at 37 degree C.
No detectable conversion of covalently closed circular DNA to nicked DNA was observed after incubation of 20u of Klenow Fragment, exo-. with 1ug of pBR322 DNA in 50ul of reaction buffer for 4 hours at 37 degree C.
Unit Definition
1u incorporates 10nm of total deoxynucleotides into a DE-81 absorbable form using poly(dA-dT)• poly(dA-dT) as a template-primer in 30 minutes at 37ºC
Preparation and Storage
For long-term storage, store at -20 degree C. Aliquots are stable for at least 6 months at -20 degree C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer.
Related Product Information for DNA Polymerase I Klenow Fragment, Exo-Minus recombinant protein
Klenow fragment, exo, exhibits 5'-3' polymerase activity of DNA polymerase I, E. coli. It lacks 3'-5' and 5'-3' exonuclease activities. It is purified from an E. coli strain carrying a modified DNA polymerase I large fragment overproducing plasmid. Klenow Fragment, exo-, is not recommended for fill-in reactions prior to DNA ligation. It frequently adds one or more extra nucleotides to the 3'terminus of a blunt-ended DNA substrate in a non-template directed fashion.
Product Categories/Family for DNA Polymerase I Klenow Fragment, Exo-Minus recombinant protein
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Product Notes
The DNA Polymerase I Klenow Fragment, Exo-Minus (Catalog #AAA637734) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. Suitable for use in labeling of DNA by the random primer method; labeling recessed 3'-termini of double-stranded DNA; DNA sequencing by the method of Sanger, F., et al.; Strand Displacement Amplification (SDA). Researchers should empirically determine the suitability of the DNA Polymerase I Klenow Fragment, Exo-Minus for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "DNA Polymerase I Klenow Fragment, Exo-Minus, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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