Rabbit anti-Mouse, Rat NOGOA Polyclonal Antibody | anti-NOGOA antibody

RABBIT ANTI HUMAN NOGOA (N-TERMINAL)

Cat. Num. AAA223852

• Gene Names: RTN4; ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• Reactivity: Mouse, Rat
• Applications: Immunohistochemistry, Western Blot
• Synonyms: NOGOA; Polyclonal Antibody; RABBIT ANTI HUMAN NOGOA (N-TERMINAL); anti-NOGOA antibody

• Host: Rabbit
• Reactivity: Mouse, Rat
• Clonality: Polyclonal
• Isotype: IgG
• Form/Format: Purified; Purified IgG - liquid
• Concentration: IgG concentration 1.0mg/ml (varies by lot)
• Sequence Length: 199

• Applicable Applications for anti-NOGOA antibody: Immunohistology Paraffin*, Western Blot (WB)
• Application Notes: Western Blot: This item detects a band of approximately 180 kDa in Mouse Brain tissue lysate.; Immunohistology - Paraffin: Maximum Dilution: 2.5ug/ml; Application Note: This product requires antigen retrieval using heat treatment prior to staining of paraffin sections. Sodium citrate buffer pH 6.0 is recommended for this purpose.; Western Blotting: Minimum Dilution: 0.5; Maximum Dilution: 1.0ug/ml
• Perservative Stabilisers: 0.02% Sodium Azide (NaN3)
• Histology Positive Control Tissue: Mouse brain; Buffer Solution
• Antiserum Preparation: Antisera to human NogoA were raised by repeated immunisation of rabbits with highly purified antigen. Purified IgG prepared from whole serum by affinity chromatography.
• Target Species: Human
• Preparation and Storage: Store at 4 degree C or at -20 degree C if preferred. Storage in frost-free freezers is not recommended. This product should be stored undiluted. Avoid repeated freezing and thawing as this may denature the antibody. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 18 months from date of despatch.

Testing Data #1

(Western blot analysis of mouse brain lysate probed with Rabbit anti Human NogoA (MBS223852) at 0.5(A) and 1(B) ug/ml)

Testing Data #2

(Immunohistochemical staining of mouse brain with Rabbit anti Human NogoA (MBS223852) )

Related Product Information for anti-NOGOA antibody

Rabbit anti Human NOGOA antibody recognizes human Reticulon-4 (RTN4), also known as NogoA, neurite outgrowth inhibitor, Foocen, Neuroendocrine-specific protein, Neuroendocrine-specific protein C homolog, Reticulon-5 or RTN-x. NogoA is a 1192 amino acid multi pass transmembrane protein associated with the endoplasmic reticulum, a member of a family of integral membrane proteins termed reticulons. Six isoforms of NogoA can be generated by alternative splicing, the canonical isoform 1 is predominantly expressed in the brain and testis with weaker expression in the heart and skeletal muscle. Reticulons are involved in various neurodegenerative diseases such as Amyotrophic lateral sclerosis,and multiple sclerosis (Chiurchiù et al. 2014). Reticulon proteins have been demonstrated to regulate many cellular processes and interact with multiple proteins and receptors such as BACE. NogoA was initially identified as a myelin-associated neurite outgrowth inhibitor (Niederöst et al. 2002). NogoA is highly expressed in oligodendrocytes in the white matter of the CNS (Kuhlmann et al. 2008). Blocking NogoA activity with antibodies or other factors results in improved long distance axonal regeneration and functional recovery in experimental CNS lesion models (Schwab 2004). NOGOA has a predicted molecular weight of 130kDa however, despite its predicted molecular weight, NogoA typically migrates at ~180kDa in an SDS-PAGE. Rabbit anti human NOGOA antibody is expected to recognize all isoforms of NogoA.

NCBI and Uniprot Product Information

• NCBI GI #: 5902016
• NCBI GeneID: 57142
• NCBI Accession #: NP_008939.1
• NCBI GenBank Nucleotide #: NM_007008.2
• UniProt Accession #: Q9NQC3; O94962; Q7L7Q5; Q7L7Q6; Q7L7Q8; Q8IUA4; Q96B16; Q9BXG5; Q9H212; Q9H3I3; Q9UQ42; Q9Y293
• Molecular Weight: 108,450 Da
• NCBI Official Full Name: reticulon-4 isoform C
• NCBI Official Synonym Full Names: reticulon 4
• NCBI Official Symbol: RTN4
• NCBI Official Synonym Symbols: ASY; NSP; NOGO; NOGOC; RTN-X; NOGO-A; NSP-CL; Nogo-B; Nogo-C; RTN4-A; RTN4-C; RTN4-B1; RTN4-B2; NI220/250; Nbla00271; Nbla10545
• NCBI Protein Information: reticulon-4; Human NogoA; My043 protein; foocen; neurite growth inhibitor 220; neurite outgrowth inhibitor; neuroendocrine-specific protein C homolog; reticulon 5
• UniProt Protein Name: Reticulon-4
• UniProt Gene Name: RTN4
• UniProt Synonym Gene Names: KIAA0886; NOGO; Nogo protein; NSP
• UniProt Entry Name: RTN4_HUMAN

NCBI Description

This gene belongs to the family of reticulon encoding genes. Reticulons are associated with the endoplasmic reticulum, and are involved in neuroendocrine secretion or in membrane trafficking in neuroendocrine cells. The product of this gene is a potent neurite outgrowth inhibitor which may also help block the regeneration of the central nervous system in higher vertebrates. Alternatively spliced transcript variants derived both from differential splicing and differential promoter usage and encoding different isoforms have been identified. [provided by RefSeq, Jul 2008]

Uniprot Description

Nogo: Developmental neurite growth regulatory factor with a role as a negative regulator of axon-axon adhesion and growth, and as a facilitator of neurite branching. Regulates neurite fasciculation, branching and extension in the developing nervous system. Involved in down-regulation of growth, stabilization of wiring and restriction of plasticity in the adult CNS. Regulates the radial migration of cortical neurons via an RTN4R-LINGO1 containing receptor complex. Isoform 2 reduces the anti-apoptotic activity of Bcl-xl and Bcl-2. This is likely consecutive to their change in subcellular location, from the mitochondria to the endoplasmic reticulum, after binding and sequestration. Isoform 2 and isoform 3 inhibit BACE1 activity and amyloid precursor protein processing. 6 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, multi-pass; Motility/polarity/chemotaxis; Inhibitor; Cell development/differentiation; Membrane protein, integral; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 2p16.3

Cellular Component: cell projection; cell soma; endoplasmic reticulum; plasma membrane; integral to endoplasmic reticulum membrane; nuclear envelope; intracellular

Molecular Function: protein binding

Biological Process: regulation of apoptosis; nerve growth factor receptor signaling pathway; cerebral cortex radial glia guided migration; apoptosis; axonal fasciculation; negative regulation of axonogenesis; negative regulation of cell growth; angiogenesis; negative regulation of axon extension; regulation of axonogenesis; regulation of cell migration