Human Type I iodothyronine deiodinase ELISA Kit | DIO1 elisa kit

Human Type I iodothyronine deiodinase ELISA Kit

Cat. Num. AAA2884007

• Gene Names: DIO1; 5DI; TXDI1
• Reactivity: Human
• Synonyms: Type I iodothyronine deiodinase; Human Type I iodothyronine deiodinase ELISA Kit; 5DI; DIOI; Type 1 DI; Type-I 5'-deiodinase; DIO1; ITDI1; TXDI1; 1.97.1.10; DIO1 elisa kit

• Reactivity: Human
• Sequence Length: 249

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for DIO1 elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557522
• NCBI GeneID: 1733
• NCBI Accession #: NP_000783.2
• NCBI GenBank Nucleotide #: NM_000792.5
• UniProt Accession #: P49895; Q1RN02; Q3KNP8; Q6Q4C1; Q6Q4C2; Q6Q4C3; Q6Q4C4; Q6Q4C5; Q6Q4C6; Q6Q4C7; Q6Q4C9; Q8WWC6
• Molecular Weight: 9,774 Da
• NCBI Official Full Name: type I iodothyronine deiodinase isoform a
• NCBI Official Synonym Full Names: deiodinase, iodothyronine, type I
• NCBI Official Symbol: DIO1
• NCBI Official Synonym Symbols: 5DI; TXDI1
• NCBI Protein Information: type I iodothyronine deiodinase
• UniProt Protein Name: Type I iodothyronine deiodinase
• Protein Family: Type I iodothyronine deiodinase
• UniProt Gene Name: DIO1
• UniProt Synonym Gene Names: ITDI1; TXDI1
• UniProt Entry Name: IOD1_HUMAN

NCBI Description

The protein encoded by this gene is a thiol-requiring propylthiouracil-sensitive oxidoreductase. It activates thyroid hormone by converting the prohormone thyroxine (T4) by outer ring deiodination (ORD) to bioactive 3,3',5-triiodothyronine (T3). It also degrades both hormones by inner ring deiodination (IRD). Alternative splicing results in multiple transcript variants encoding different isoforms. Some, but not all, isoforms contain a selenocysteine (Sec) residue encoded by the UGA codon, which normally signals translation termination. The 3' UTR of Sec-containing genes have a common stem-loop structure, the sec insertion sequence (SECIS), which is necessary for the recognition of UGA as a Sec codon rather than as a stop signal. Additional transcript variants have been described but are not supported by experimental evidence. [provided by RefSeq, Jul 2008]

Uniprot Description

DIO1: Responsible for the deiodination of T4 (3,5,3',5'- tetraiodothyronine) into T3 (3,5,3'-triiodothyronine) and of T3 into T2 (3,3'-diiodothyronine). Plays a role in providing a source of plasma T3 by deiodination of T4 in peripheral tissues such as liver and kidney. Belongs to the iodothyronine deiodinase family. 9 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Oxidoreductase; EC 1.97.1.10

Chromosomal Location of Human Ortholog: 1p33-p32

Cellular Component: endoplasmic reticulum membrane; integral to membrane; plasma membrane

Molecular Function: selenium binding; thyroxine 5'-deiodinase activity

Biological Process: hormone biosynthetic process; thyroid hormone generation