Serum Diluent
General Serum Diluent
Synonyms
Serum Diluent; General Serum Diluent; Serum Diluent diluent
Form/Format
Supplied at 1X.
Application Notes
1. Prepare standards and samples in General Serum Diluent.
2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL.
4. Highly concentrated samples may need to be diluted 1:1,000 or more.
5. Once diluted, run the assay according to the specific ELISA protocol.
2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL.
4. Highly concentrated samples may need to be diluted 1:1,000 or more.
5. Once diluted, run the assay according to the specific ELISA protocol.
pH
7.4 at 1X
Contains
Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Serum Diluent diluent
Description: General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. General Serum Diluent can also be used for dilution of many types of serum samples, including mammalian and chicken, tested in antigen-down ELISA formats.
Background: General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. This product can also be used for dilution of many types of serum samples, including mammalian and chicken, tested in antigen-down ELISA formats.
Hyper-immunized serum samples contain nonspecific IgG antibody that may interfere with the titration. Proprietary additives have been incorporated into the General Serum Diluent formulation to minimize nonspecific binding interactions of the nonspecific IgG in the samples, thus decreasing assay background noise. General Serum Diluent may also be used for dilutions of the antigen standard for use in antibody capture sandwich ELISA formats.
Background: General Serum Diluent is formulated for diluting goat, rabbit, mouse, avian, and human sera, as well as mouse ascites fluid and cell culture media, for testing in sandwich ELISA configurations. This product can also be used for dilution of many types of serum samples, including mammalian and chicken, tested in antigen-down ELISA formats.
Hyper-immunized serum samples contain nonspecific IgG antibody that may interfere with the titration. Proprietary additives have been incorporated into the General Serum Diluent formulation to minimize nonspecific binding interactions of the nonspecific IgG in the samples, thus decreasing assay background noise. General Serum Diluent may also be used for dilutions of the antigen standard for use in antibody capture sandwich ELISA formats.
Product Categories/Family for Serum Diluent diluent
Similar Products
Product Notes
The Serum Diluent (Catalog #AAA258402) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. Prepare standards and samples in General Serum Diluent. 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. 3. To dilute the sample 1:100, add 1 part sample to 99 parts General Serum Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL. 4. Highly concentrated samples may need to be diluted 1:1,000 or more. 5. Once diluted, run the assay according to the specific ELISA protocol. Researchers should empirically determine the suitability of the Serum Diluent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Serum Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
