Neptune Sample Diluent
Neptune Sample Diluent
Synonyms
Neptune Sample Diluent; Neptune Sample Diluent diluent
Form/Format
Supplied at 1X.
Application Notes
1. Prepare standards and samples in Neptune Sample Diluent.
2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
3. To dilute the sample 1:100, add 1 part sample to 99 parts Neptune Sample Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL.
4. Highly concentrated samples may need to be diluted 1:1,000 or more.
5. Once diluted, run the assay according to the specific ELISA protocol.
2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding.
3. To dilute the sample 1:100, add 1 part sample to 99 parts Neptune Sample Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL.
4. Highly concentrated samples may need to be diluted 1:1,000 or more.
5. Once diluted, run the assay according to the specific ELISA protocol.
pH
7.4 at 1X
Contains
Non-Mammalian Proteins.
Preparation and Storage
Store at 2-8 degree C.
Related Product Information for Neptune Sample Diluent diluent
Description: Neptune Sample Diluent is a non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine sources in an antigen-down format. It can also be used for human or other mammalian plasma samples in antibody-sandwich ELISAs.
Background: Neptune Sample Diluent is a non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine sources in an antigen-down format. It can also be used for human or other mammalian plasma samples in antibody-sandwich ELISAs.
In certain animal species, nonspecific adsorption of serum IgG to antigen coated and blocked ELISA plate surfaces is a common problem. Utilization of Neptune Sample Diluent as the serum or plasma test sample diluent will minimize the plate well adsorption of nonspecific IgGs that are always present in serum or plasma samples.
Neptune Sample Diluent contains proprietary additives in a buffered PBS base to provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. This diluent also contains an antimicrobial agent to provide a solution free of bacterial contamination.
Background: Neptune Sample Diluent is a non-mammalian protein-containing solution highly recommended for use with serum or plasma samples from mouse, porcine or bovine sources in an antigen-down format. It can also be used for human or other mammalian plasma samples in antibody-sandwich ELISAs.
In certain animal species, nonspecific adsorption of serum IgG to antigen coated and blocked ELISA plate surfaces is a common problem. Utilization of Neptune Sample Diluent as the serum or plasma test sample diluent will minimize the plate well adsorption of nonspecific IgGs that are always present in serum or plasma samples.
Neptune Sample Diluent contains proprietary additives in a buffered PBS base to provide an assay environment that minimizes nonspecific binding while maintaining proper ionic strength and pH for efficient antibody-antigen interaction. This diluent also contains an antimicrobial agent to provide a solution free of bacterial contamination.
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Product Notes
The Neptune Sample Diluent (Catalog #AAA258380) is a Diluent and is intended for research purposes only. The product is available for immediate purchase. 1. Prepare standards and samples in Neptune Sample Diluent. 2. Serum samples should generally be diluted at least 1:50 in order to minimize backgrounds caused by non-specific antibody binding. 3. To dilute the sample 1:100, add 1 part sample to 99 parts Neptune Sample Diluent. For example, add 10 muL sample to 990 muL sample diluent for a total of 1,000 muL. 4. Highly concentrated samples may need to be diluted 1:1,000 or more. 5. Once diluted, run the assay according to the specific ELISA protocol. Researchers should empirically determine the suitability of the Neptune Sample Diluent for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "Neptune Sample Diluent, Diluent" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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