Probable diguanylate cyclase YcdT (ycdT) Recombinant Protein | ycdT recombinant protein

Recombinant Escherichia coli Probable diguanylate cyclase YcdT (ycdT), partial

Gene Names

ycdT; ECK1015; JW5143

Purity

Greater or equal to 85% purity as determined by SDS-PAGE.

Synonyms

Probable diguanylate cyclase YcdT (ycdT); Recombinant Escherichia coli Probable diguanylate cyclase YcdT (ycdT); partial; ycdT recombinant protein

Ordering
Cat. Num. AAA1146440 When autocomplete results are available use up and down arrows to review and enter to select. Touch device users, explore by touch or with swipe gestures. For Research Use Only!

Host

E Coli or Yeast or Baculovirus or Mammalian Cell

Purity/Purification

Greater or equal to 85% purity as determined by SDS-PAGE.

Form/Format

Lyophilized or liquid (Format to be determined during the manufacturing process)

Sequence Positions

1-452

Sequence

MEKDYLRISSTVLVSLLFGLALVLVNSWFNQPGVEEVVPRSTYLMVMIALFFIDTVAFIFMQLYFIYDRRQFSNCVLSLAFLSCLIYFVITVIIIQQIIEERLTSSVVQNDIAIYYLFRQMSLCILIFLALVNKVSENTKQRNLFSKKMTLCISLFFVFGGPIVAHILSSHYESYNLHIAELTNENGQVVWKASYVTIMIFMWLTLLSVNLYFNGLRYDIWNGVTVIAFCAVLYNISLLFMSRYSVSTWYISRTIEVVSKLTVMVIFMCHIFSALRVTKNIAHRDPLTNIFNRNYFFNELTVQSASAQKTPYCVMIMDIDHFKKVNDTWGHPVGDQVIKTVVNIIGKSIRPDDLLARVGGEEFGVLLTDIDTERAKALAERIRENVERLTGDNPEYAIPQKVTISIGAVVTQENALNPNEIYRLADNALYEAKETGRNKVVVRDVVNFCESP

Sequence Length

452

Species

Escherichia coli (strain K12)

Preparation and Storage

Store at -20 degrees C. For long-term storage, store at -20 degrees C or -80 degrees C. Store working aliquots at 4 degrees C for up to one week. Repeated freezing and thawing is not recommended.

NCBI and Uniprot Product Information

NCBI GI #

16128989

NCBI GeneID

945593

NCBI Accession #

NP_415544.1

NCBI GenBank Nucleotide #

NC_000913.3

UniProt Accession #

P75908; Q9R7P4; Q9R7P6

Molecular Weight

51,783 Da

NCBI Official Full Name

diguanylate cyclase, membrane-anchored

NCBI Official Symbol

ycdT

NCBI Official Synonym Symbols

ECK1015; JW5143

NCBI Protein Information

diguanylate cyclase, membrane-anchored

UniProt Protein Name

Probable diguanylate cyclase DgcT

Protein Family

Probable diguanylate cyclase

UniProt Gene Name

dgcT

UniProt Synonym Gene Names

DGC

NCBI Description

YcdT mRNA is translationally repressed by CsrA (Jonas, 2008). ycdT overexpression causes a 20-fold increase in di-c-GMP and represses motility, but a mutant has little effect (Jonas, 2008). [More information is available at EcoGene: EG13866]. YcdT is a diguanylate cyclase that regulates motility; the effect on motility is probably mediated by the second messenger c-di-GMP . [More information is available at EcoCyc: G6532].

Uniprot Description

Probably catalyzes the synthesis of cyclic-di-GMP (c-di-GMP) via the condensation of 2 GTP molecules. Overexpression leads to a strong repression of swimming; swimming returns to normal when residues 359-360 are both mutated to Ala. Overexpression also leads to a 20-fold increase in c-di-GMP levels in vivo. Cyclic-di-GMP is a second messenger which controls cell surface-associated traits in bacteria.

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Product Notes

The ycdT dgct (Catalog #AAA1146440) is a Recombinant Protein produced from E Coli or Yeast or Baculovirus or Mammalian Cell and is intended for research purposes only. The product is available for immediate purchase. The immunogen sequence is 1-452. The amino acid sequence is listed below: MEKDYLRISS TVLVSLLFGL ALVLVNSWFN QPGVEEVVPR STYLMVMIAL FFIDTVAFIF MQLYFIYDRR QFSNCVLSLA FLSCLIYFVI TVIIIQQIIE ERLTSSVVQN DIAIYYLFRQ MSLCILIFLA LVNKVSENTK QRNLFSKKMT LCISLFFVFG GPIVAHILSS HYESYNLHIA ELTNENGQVV WKASYVTIMI FMWLTLLSVN LYFNGLRYDI WNGVTVIAFC AVLYNISLLF MSRYSVSTWY ISRTIEVVSK LTVMVIFMCH IFSALRVTKN IAHRDPLTNI FNRNYFFNEL TVQSASAQKT PYCVMIMDID HFKKVNDTWG HPVGDQVIKT VVNIIGKSIR PDDLLARVGG EEFGVLLTDI DTERAKALAE RIRENVERLT GDNPEYAIPQ KVTISIGAVV TQENALNPNE IYRLADNALY EAKETGRNKV VVRDVVNFCE SP. It is sometimes possible for the material contained within the vial of "Probable diguanylate cyclase YcdT (ycdT), Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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