Rabbit anti-Human RCHY1 Polyclonal Antibody | anti-RCHY1 antibody

RCHY1 (RING Finger and CHY Zinc Finger Domain-containing Protein 1, Androgen Receptor N-terminal-interacting Protein, CH-rich-interacting Match with PLAG1, E3 Ubiquitin-protein Ligase Pirh2, RING Finger Protein 199, Zinc Finger Protein 363, p53-induced RI

Cat. Num. AAA6392235

• Gene Names: RCHY1; ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
• Reactivity: Human
• Applications: Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: RCHY1; Polyclonal Antibody; RCHY1 (RING Finger and CHY Zinc Finger Domain-containing Protein 1; Androgen Receptor N-terminal-interacting Protein; CH-rich-interacting Match with PLAG1; E3 Ubiquitin-protein Ligase Pirh2; RING Finger Protein 199; Zinc Finger Protein 363; p53-induced RI; anti-RCHY1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human RCHY1.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Fluorescein Isothiocyanate (FITC).

• Applicable Applications for anti-RCHY1 antibody: Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Full length human RCHY1, aa1-252 (NP_001009922.1).
• Immunogen Sequence: MAATAREDGASGQERGQRGCEHYDRGCLLKAPCCDKLYTCRLCHDNNEDHQLDRFKVKEVQCINCEKIQHAQQTCEECSTLFGEYYCDICHLFDKDKKQYHCENCGICRIGPKEDFFHCLKCNLCLAMNLQGRHKCIENVSRQNCPICLEDIHTSRVVAHVLPCGHLLHRGYRCPLCMHSALDMTRYWRQLDDEVAQTPMPSEYQNMTVDILCNDCNGRSTVQFHILGMKCKICESYNTAQAGGRRISLDQQ
• Conjugate: FITC
• Note: Preservative Free
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Caution: FITC conjugates are sensitive to light. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(RCHY1 rabbit polyclonal antibody. Western Blot analysis of RCHY1 expression in human liver.)

Western Blot (WB)

(Western Blot analysis of RCHY1 expression in transfected 293T cell line by RCHY1 polyclonal antibody. Lane 1: RCHY1 transfected lysate (29kD). Lane 2: Non-transfected lysate.)

Related Product Information for anti-RCHY1 antibody

RCHY1 protein has ubiquitin-protein ligase activity. This protein binds with p53 and promotes the ubiquitin-mediated proteosomal degradation of p53. Gene of RCHY1 is oncogenic because loss of p53 function contributes directly to malignant tumor development.

Product Categories/Family for anti-RCHY1 antibody

Antibodies; Transcription Factors; Zinc (Ring) Finger

NCBI and Uniprot Product Information

• NCBI GI #: 58331199
• NCBI GeneID: 25898
• NCBI Accession #: NP_001009922.1
• NCBI GenBank Nucleotide #: NM_001009922.1
• UniProt Accession #: Q96PM5; Q2KN33; Q59GN7; Q86X26; B3KRG3; C7E541; C7E542; C7E543; D3YRV2; E7EMC8; E7ETW5; J3KPI0
• Molecular Weight: 24,210 Da
• NCBI Official Full Name: RING finger and CHY zinc finger domain-containing protein 1 isoform 3
• NCBI Official Synonym Full Names: ring finger and CHY zinc finger domain containing 1, E3 ubiquitin protein ligase
• NCBI Official Symbol: RCHY1
• NCBI Official Synonym Symbols: ZCHY; ARNIP; CHIMP; PIRH2; RNF199; ZNF363; PRO1996
• NCBI Protein Information: RING finger and CHY zinc finger domain-containing protein 1; CH-rich interacting match with PLAG1; E3 ubiquitin-protein ligase Pirh2; RING finger protein 199; androgen-receptor N-terminal-interacting protein; p53-induced protein with a RING-H2 domain; zin
• UniProt Protein Name: RING finger and CHY zinc finger domain-containing protein 1
• Protein Family: RING finger and CHY zinc finger domain-containing protein
• UniProt Gene Name: RCHY1
• UniProt Synonym Gene Names: ARNIP; CHIMP; PIRH2; RNF199; ZNF363; hPirh2
• UniProt Entry Name: ZN363_HUMAN

NCBI Description

The protein encoded by this gene has ubiquitin ligase activity. It mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including tumor protein 53, histone deacetylase 1, and cyclin-dependent kinase inhibitor 1B, thus regulating their levels and cell cycle progression. Alternatively spliced transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jun 2013]

Uniprot Description

RCHY1: Mediates E3-dependent ubiquitination and proteasomal degradation of target proteins, including p53/TP53, HDAC1 and p27Kip1. Preferentially acts on tetrameric p53/TP53. Contributes to the regulation of p27Kip1 and p53/TP53 levels, and thereby contributes to the regulation of the cell cycle progression. Increases AR transcription factor activity. Monomer and homodimer. Interacts with AR, p53/TP53, MDM2, HDAC1, KAT5, PLAG1, PLAGL2, p27Kip1, COPE, UBE2D2 and GORAB/NTKLBP1. Up-regulated during the S phase of the cell cycle. Expressed at low levels during G phase. 3 isoforms of the human protein are produced by alternative splicing.

Protein type: Ubiquitin ligase; Ubiquitin conjugating system; Nuclear receptor co-regulator; EC 6.3.2.-; EC 6.3.2.19

Chromosomal Location of Human Ortholog: 4q21.1

Cellular Component: nucleoplasm; cytoplasm; nuclear speck; nucleus; ubiquitin ligase complex

Molecular Function: protein binding; protein homodimerization activity; zinc ion binding; p53 binding; ubiquitin-protein ligase activity; ligase activity; receptor binding

Biological Process: protein autoubiquitination; positive regulation of protein ubiquitination; positive regulation of proteasomal ubiquitin-dependent protein catabolic process; protein ubiquitination during ubiquitin-dependent protein catabolic process; protein ubiquitination