Human Serotransferrin ELISA Kit | TF elisa kit

Human Serotransferrin ELISA Kit

Cat. Num. AAA2883733

• Gene Names: TF; TFQTL1; PRO1557; PRO2086; HEL-S-71p
• Reactivity: Human
• Synonyms: Serotransferrin; Human Serotransferrin ELISA Kit; Transferrin; Beta-1 metal-binding globulin; Siderophilin; TF; PRO1400; TF elisa kit

• Reactivity: Human
• Sequence Length: 698

• Samples: Serum, Plasma, Tissue Homogenates, Cell Culture Supernates Or Other Biological Fluids.
• Preparation and Storage: For long term storage, please store the entire kit at -20 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for TF elisa kit

Intended Uses: This immunoassay kit allows for the in vitro quantitative determination of target antigen concentrations in serum, plasma, tissue homogenates, cell culture supernates or other biological fluids.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to target antigen. Standards or samples are then added to the appropriate microtiter plate wells with a biotin-conjugated antibody preparation specific for target antigen and then avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB substrate solution is added to each well. Only those wells that contain target antigen, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of target antigen in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 4557871
• NCBI GeneID: 7018
• NCBI Accession #: NP_001054.1
• NCBI GenBank Nucleotide #: NM_001063.3
• UniProt Accession #: P02787; O43890; Q1HBA5; Q9NQB8; Q9UHV0
• Molecular Weight: 77,064 Da
• NCBI Official Full Name: serotransferrin
• NCBI Official Synonym Full Names: transferrin
• NCBI Official Symbol: TF
• NCBI Official Synonym Symbols: TFQTL1; PRO1557; PRO2086; HEL-S-71p
• NCBI Protein Information: serotransferrin
• UniProt Protein Name: Serotransferrin
• Protein Family: Serotransferrin
• UniProt Gene Name: TF
• UniProt Synonym Gene Names: Transferrin
• UniProt Entry Name: TRFE_HUMAN

NCBI Description

This gene encodes a glycoprotein with an approximate molecular weight of 76.5 kDa. It is thought to have been created as a result of an ancient gene duplication event that led to generation of homologous C and N-terminal domains each of which binds one ion of ferric iron. The function of this protein is to transport iron from the intestine, reticuloendothelial system, and liver parenchymal cells to all proliferating cells in the body. This protein may also have a physiologic role as granulocyte/pollen-binding protein (GPBP) involved in the removal of certain organic matter and allergens from serum. [provided by RefSeq, Sep 2009]

Uniprot Description

TF: Transferrins are iron binding transport proteins which can bind two Fe(3+) ions in association with the binding of an anion, usually bicarbonate. It is responsible for the transport of iron from sites of absorption and heme degradation to those of storage and utilization. Serum transferrin may also have a further role in stimulating cell proliferation. Defects in TF are the cause of atransferrinemia (ATRAF). Atransferrinemia is rare autosomal recessive disorder characterized by iron overload and hypochromic anemia. Belongs to the transferrin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 3q22.1

Cellular Component: apical plasma membrane; basal plasma membrane; cell surface; coated pit; cytoplasmic membrane-bound vesicle; early endosome; endocytic vesicle; endosome membrane; extracellular region; extracellular space; extrinsic to external side of plasma membrane; late endosome; perinuclear region of cytoplasm; recycling endosome; vesicle

Molecular Function: ferric iron binding; ferric iron transmembrane transporter activity; ferrous iron binding; protein binding

Biological Process: blood coagulation; cellular iron ion homeostasis; iron ion homeostasis; platelet activation; platelet degranulation; positive regulation of receptor-mediated endocytosis; regulation of protein stability; retinal homeostasis; transferrin transport; transmembrane transport

Disease: Atransferrinemia