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Typical Testing Data/Standard Curve (for reference only)

Mouse defensin, alpha 4, corticostatin ELISA Kit | DEFA4 elisa kit

Mouse Neutrophil defensin 4, DEFA4 ELISA Kit

Gene Names
Defa4; Crp4; Defa28; Defcr4
Reactivity
Mouse
Synonyms
defensin; alpha 4; corticostatin; Mouse Neutrophil defensin 4; DEFA4 ELISA Kit; Mouse Neutrophil defensin 4 (DEFA4) ELISA kit; DEF4; HNP-4; HP-4; HP4; MGC120099; MGC138296; preproprotein; DEFA4 elisa kit
Ordering
For Research Use Only!
Reactivity
Mouse
Specificity
This assay has high sensitivity and excellent specificity for detection of mouse DEFA4. No significant cross-reactivity or interference between mouse DEFA4 and analogues was observed.
Sequence Length
92
Samples
Serum, plasma, tissue homogenates, cell lysates
Assay Type
Quantitative Sandwich
Detection Range
0.312 ng/ml-20 ng/ml
Sensitivity
< 0.078 ng/ml
Intra-assay Precision
Intra-assay Precision (Precision within an assay): CV%<8%. Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision
Inter-assay Precision (Precision between assays): CV%<10%. Three samples of known concentration were tested in twenty assays to assess.
Preparation and Storage
Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DEFA4 elisa kit
Principle of the Assay: This assay employs the quantitative sandwich enzyme immunoassay technique. Antibody specific for DEFA4 has been pre-coated onto a microplate. Standards and samples are pipetted into the wells and any DEFA4 present is bound by the immobilized antibody. After removing any unbound substances, a biotin-conjugated antibody specific for DEFA4 is added to the wells. After washing, avidin conjugated Horseradish Peroxidase (HRP) is added to the wells. Following a wash to remove any unbound avidin-enzyme reagent, a substrate solution is added to the wells and color develops in proportion to the amount of DEFA4 bound in the initial step. The color development is stopped and the intensity of the color is measured.

NCBI and Uniprot Product Information

NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
10,272 Da
NCBI Official Full Name
alpha-defensin 4
NCBI Official Synonym Full Names
defensin, alpha, 4
NCBI Official Symbol
Defa4
NCBI Official Synonym Symbols
Crp4; Defa28; Defcr4
NCBI Protein Information
alpha-defensin 4; alpha-defensin, 28; defensin, alpha, 28; defensin related cryptdin 4; defensin-related cryptdin-4
UniProt Protein Name
Alpha-defensin 4
Protein Family
UniProt Gene Name
Defa4
UniProt Synonym Gene Names
Defcr4
UniProt Entry Name
DEFA4_MOUSE

Uniprot Description

DEFA4: Has antimicrobial activity against Gram-negative bacteria, and to a lesser extent also against Gram-positive bacteria and fungi. Protects blood cells against infection with HIV-1 (in vitro). Inhibits corticotropin (ACTH)-stimulated corticosterone production. Belongs to the alpha-defensin family.

Protein type: Secreted, signal peptide; Secreted

Cellular Component: extracellular space; extracellular region

Molecular Function: phospholipid binding

Biological Process: defense response to bacterium; antibacterial humoral response; defense response; membrane disruption in another organism

Research Articles on DEFA4

Similar Products

Product Notes

The Mouse DEFA4 defa4 (Catalog #AAA904696) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA904696 ELISA Kit recognizes Mouse DEFA4. It is sometimes possible for the material contained within the vial of "defensin, alpha 4, corticostatin, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.

Precautions

All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.

Disclaimer

Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.

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