Western Blot (WB)
(Figure 1: Western blot analysis using NACC1 mAb against HEK293 (1) and NACC1(AA: 165-438)-hIgGFc transfected HEK293 (2) cell lysate.)
Mouse anti-Human NACC1 Monoclonal Antibody | anti-NACC1 antibody
NACC1 Antibody
Gene Names
NACC1; NAC1; BEND8; NAC-1; NECFM; BTBD30; BTBD14B
Reactivity
Human
Applications
Western Blot, Immunohistochemistry, ELISA
Purity
Affinity-Chromatography
Synonyms
NACC1; Monoclonal Antibody; NACC1 Antibody; BEND8; BTB Domain Containing 14B; BTB/POZ domain-containing protein 14B; btbd14b; FLJ37383; NAC-1; NAC1; Nacc1; NACC1_HUMAN; Nucleus accumbens-associated protein 1; anti-NACC1 antibody
Host
Mouse
Reactivity
Human
Clonality
Monoclonal
Isotype
IgG1
Specificity
NACC1 antibody detects endogenous levels of total NACC1
Purity/Purification
Affinity-Chromatography
Form/Format
Phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
Concentration
1mg/ml (varies by lot)
Sequence Length
527
Applicable Applications for anti-NACC1 antibody
Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA)
Application Notes
ELISA: 1:10000
WB: 1:500-1:2000
IHC: 1:200-1:1000
WB: 1:500-1:2000
IHC: 1:200-1:1000
Immunogen
Purified recombinant fragment of human NACC1 expressed in E. Coli
Subcellular Location
Nucleus. Cytoplasm. Distribution in the cytoplasm is dependent on phosphorylation.
Tissue Specificity
Overexpressed in several types of carcinomas including ovarian serous carcinomas. Expression levels positively correlate with tumor recurrence in ovarian serous carcinomas, and intense immunoreactivity in primary ovarian tumors predicts early recurrence. Up-regulated in ovarian carcinomas after chemotherapy, suggesting a role in development of chemotherapy resistance in ovarian cancer.
Preparation and Storage
Store at -20 degree C. Stable for 12 months from date of receipt.
Western Blot (WB)
(Figure 1: Western blot analysis using NACC1 mAb against HEK293 (1) and NACC1(AA: 165-438)-hIgGFc transfected HEK293 (2) cell lysate.)
Western Blot (WB)
(Figure 1: Western blot analysis using NACC1 mAb against HEK293 (1) and NACC1(AA: 165-438)-hIgGFc transfected HEK293 (2) cell lysate.)
Related Product Information for anti-NACC1 antibody
Description: NAC1 or nuclear accumbens-1 is a nuclear factor that belongs to the POZ/BTB (Pox virus and zinc finger/bric-a-brac tramtrack broad complex) domain family. Also known as BTBD14B, it was originally identified in a unique neuronal forebrain structure responsible for reward motivation and addictive behaviors. NAC1 recruits HDAC3 and HDAC4 to transcriptionally repress gene expression in neuronal cells (3) and specifically co-represses other POZ/BTB proteins in the central nervous system. NAC1 is upregulated in several tumor types, including breast, renal cell, and hepatocellular carcinoma, as well as high grade ovarian serous carcinoma, where it has long been suspected as a chemoresistance gene. The chemoresistance mechanism reportedly occurs through NAC1 negative regulation of the GADD45 pathway. NAC1 has also been described as part of the extended transcriptional network in pluripotent cells that involves Oct-4, Sox2, Nanog, Sall1, KLF4 and Sall4.
Function: Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Subunit Structure: Homooligomer; mediated by the BTB domain. Interacts with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1.
Function: Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Subunit Structure: Homooligomer; mediated by the BTB domain. Interacts with HDAC3 and HDAC4. Interacts (via BTB domain) with CUL3, PSMD7 AND RCOR1.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
UniProt Accession #
Molecular Weight
Observed: 58 kDa
Predicted: 58 kDa
Predicted: 58 kDa
NCBI Official Full Name
nucleus accumbens-associated protein 1
NCBI Official Synonym Full Names
nucleus accumbens associated 1
NCBI Official Symbol
NACC1
NCBI Official Synonym Symbols
NAC1; BEND8; NAC-1; NECFM; BTBD30; BTBD14B
NCBI Protein Information
nucleus accumbens-associated protein 1
UniProt Protein Name
Nucleus accumbens-associated protein 1
Protein Family
UniProt Gene Name
NACC1
UniProt Synonym Gene Names
BTBD14B; NAC1; NAC-1
NCBI Description
This gene encodes a member of the BTB/POZ protein family. BTB/POZ proteins are involved in several cellular processes including proliferation, apoptosis and transcription regulation. The encoded protein is a transcriptional repressor that plays a role in stem cell self-renewal and pluripotency maintenance. The encoded protein also suppresses transcription of the candidate tumor suppressor Gadd45GIP1, and expression of this gene may play a role in the progression of multiple types of cancer. A pseudogene of this gene is located on the short arm of chromosome 9. [provided by RefSeq, Feb 2012]
Uniprot Description
Functions as a transcriptional repressor. Seems to function as a transcriptional corepressor in neuronal cells through recruitment of HDAC3 and HDAC4. Contributes to tumor progression, and tumor cell proliferation and survival. This may be mediated at least in part through repressing transcriptional activity of GADD45GIP1. Required for recruiting the proteasome from the nucleus to the cytoplasm and dendritic spines.
Research Articles on NACC1
Similar Products
Product Notes
The NACC1 nacc1 (Catalog #AAA9602448) is an Antibody produced from Mouse and is intended for research purposes only. The product is available for immediate purchase. The NACC1 Antibody reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's NACC1 can be used in a range of immunoassay formats including, but not limited to, Western Blot (WB), Immunohistochemistry (IHC), ELISA (EIA). ELISA: 1:10000 WB: 1:500-1:2000 IHC: 1:200-1:1000. Researchers should empirically determine the suitability of the NACC1 nacc1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. It is sometimes possible for the material contained within the vial of "NACC1, Monoclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
