Human Defensin 1, Neutrophil ELISA Kit | DEFA1 elisa kit

Human Defensin 1, Neutrophil ELISA Kit

Cat. Num. AAA16501

• Gene Names: DEFA1; HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• Reactivity: Human
• Synonyms: Defensin 1; Neutrophil; Human Defensin 1; Neutrophil ELISA Kit; DEFA1 elisa kit

• Reactivity: Human
• Specificity: No significant cross-reactivity or interference between Human DEFA1 and analogues was observed.
• Sequence Length: 94

• Samples: Serum, Plasma, Tissue Homogenate, Feces, Urine and Body Fluids
• Assay Type: Sandwich
• Detection Range: 31.2 pg/ml - 1000 pg/ml.
• Sensitivity: 5.0 pg/ml.
• Intended Uses: This Quantitative Sandwich ELISA kit is only for in vitro research use only, not for drug, household, therapeutic applications! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.
• Intra-assay Precision: Intra-assay CV (%) is less than 15%.
• Inter-assay Precision: Inter-assay CV (%) is less than 15%. [CV(%) = SD/mean ×100]
• Preparation and Storage: Store all reagents at 2-8 degree C

Related Product Information for DEFA1 elisa kit

Introduction: This Quantitative Sandwich ELISA kit is only for in vitro research use only! It is intended to be determinated DEFA1 concentrations in Human serum, plasma and other body fluids. Using Purified Human DEFA1 antibody to coat Microelisa Stripplate wells to make solid-phase antibody, then add DEFA1 and DEFA1 antibody which has been labeled with HRP to wells, then the reactants become antibody-antigen-antibody-enzyme complex, after washing completely, add TMB substrate solution, TMB substrate becomes blue color under HRP enzyme-catalyzed, reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm. The concentration of DEFA1 in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 85567619
• NCBI GeneID: 1667
• UniProt Accession #: P59665; P11479; Q14125; Q6EZF6
• Molecular Weight: 10,201 Da
• NCBI Official Full Name: Defensin, alpha 1, preproprotein
• NCBI Official Synonym Full Names: defensin, alpha 1
• NCBI Official Symbol: DEFA1
• NCBI Official Synonym Symbols: HP1; MRS; DEF1; HP-1; DEFA2; HNP-1
• NCBI Protein Information: neutrophil defensin 1; defensin, alpha 2; myeloid-related sequence; defensin, alpha 1, myeloid-related sequence
• UniProt Protein Name: Neutrophil defensin 1
• Protein Family: Alpha-defensin
• UniProt Gene Name: DEFA1
• UniProt Synonym Gene Names: DEF1; DEFA2; MRS; HP-1; HP1; HP-2; HP2
• UniProt Entry Name: DEF1_HUMAN

NCBI Description

Defensins are a family of microbicidal and cytotoxic peptides thought to be involved in host defense. They are abundant in the granules of neutrophils and also found in the epithelia of mucosal surfaces such as those of the intestine, respiratory tract, urinary tract, and vagina. Members of the defensin family are highly similar in protein sequence and distinguished by a conserved cysteine motif. The protein encoded by this gene, defensin, alpha 1, is found in the microbicidal granules of neutrophils and likely plays a role in phagocyte-mediated host defense. Several alpha defensin genes are clustered on chromosome 8. This gene differs from defensin, alpha 3 by only one amino acid. This gene and the gene encoding defensin, alpha 3 are both subject to copy number variation. [provided by RefSeq, May 2010]

Uniprot Description

defensin, alpha 1: Defensin 1 and defensin 2 have antibacterial, fungicide and antiviral activities. Has antimicrobial activity against Gram- negative and Gram-positive bacteria. Defensins are thought to kill microbes by permeabilizing their plasma membrane. Belongs to the alpha-defensin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 8p23.1

Cellular Component: extracellular matrix; extracellular space; Golgi lumen; extracellular region

Biological Process: defense response to Gram-positive bacterium; estrogen receptor signaling pathway; innate immune response in mucosa; killing of cells of another organism; antibacterial humoral response; innate immune response; immune response; chemotaxis; defense response to virus; defense response to fungus