Typical Testing Data/Standard Curve (for reference only)
Human DNA Damage Inducible Transcript 3 ELISA Kit | DDIT3 elisa kit
Human DNA Damage Inducible Transcript 3 ELISA Kit
Gene Names
ddit3.L; chop; Xchop; ddit3; gadd153
Reactivity
Human
Synonyms
DNA Damage Inducible Transcript 3; Human DNA Damage Inducible Transcript 3 ELISA Kit; DDIT3 elisa kit
Reactivity
Human
Sequence Length
211
Samples
Serum, Plasma, Cell Culture Supernatants, Body Fluid And Tissue Homogenate
Assay Type
Quantitative Competitive
Sensitivity
0.1 ng/mL.
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Related Product Information for DDIT3 elisa kit
Intended Uses: This DDIT3 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat DDIT3. This ELISA kit for research use only!
Principle of the Assay: DDIT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DDIT3 antibody and an DDIT3-HRP conjugate. The assay sample and buffer are incubated together with DDIT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDIT3 concentration since DDIT3 from samples and DDIT3-HRP conjugate compete for the anti-DDIT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDIT3 from the sample, fewer sites are left to bind DDIT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.
Principle of the Assay: DDIT3 ELISA kit applies the competitive enzyme immunoassay technique utilizing a polyclonal anti-DDIT3 antibody and an DDIT3-HRP conjugate. The assay sample and buffer are incubated together with DDIT3-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the DDIT3 concentration since DDIT3 from samples and DDIT3-HRP conjugate compete for the anti-DDIT3 antibody binding site. Since the number of sites is limited, as more sites are occupied by DDIT3 from the sample, fewer sites are left to bind DDIT3-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The DDIT3 concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
NCBI Official Full Name
DNA damage inducible transcript 3 L homeolog
NCBI Official Synonym Full Names
DNA damage inducible transcript 3 L homeolog
NCBI Official Symbol
ddit3.L
NCBI Official Synonym Symbols
chop; Xchop; ddit3; gadd153
NCBI Protein Information
DNA damage inducible transcript 3 L homeolog
Protein Family
Research Articles on DDIT3
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Product Notes
The Human DDIT3 (Catalog #AAA7254118) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA7254118 ELISA Kit recognizes Human DDIT3. It is sometimes possible for the material contained within the vial of "DNA Damage Inducible Transcript 3, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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