SDS-Page
DDAH1 recombinant protein
DDAH1, 1-285aa, Human, His tag, E Coli
Gene Names
DDAH1; DDAH; DDAHI; DDAH-1; HEL-S-16
Applications
SDS-Page
Purity
> 95% by SDS-PAGE
Synonyms
DDAH1; 1-285aa; Human; His tag; E Coli; Dimethylarginine dimethylaminohydrolase 1; DDAH; DDAHI; Dimethylargininase-1; DDAH1 recombinant protein
Host
E Coli
Purity/Purification
> 95% by SDS-PAGE
Form/Format
Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 1mM DTT, 10% glycerol, 50mM NaCl
Concentration
1 mg/ml (determined by Bradford assay) (varies by lot)
Sequence
MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS
Sequence Length
285
Applicable Applications for DDAH1 recombinant protein
SDS-PAGE
Antigen Species
Human
Tag Information
His-tag
Preparation and Storage
Can be stored at 4 degree C short term (1-2 weeks).
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
For long term storage, aliquot and store at -20 degree C or -70 degree C.
Avoid repeated freezing and thawing cycles.
SDS-Page
SDS-Page
Related Product Information for DDAH1 recombinant protein
Dimethylarginine dimethylaminohydrolase 1, also known DDAH1, belongs to the dimethylarginine dimethylaminohydrolase gene family. The DDAH1 plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. Impairment of DDAH1 causes ADMA (asymmetric dimethylarginine) accumulation and a reduction in cGMP generation. Recombinant human DDAH1 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by using conventional chromatography techniques.
Product Categories/Family for DDAH1 recombinant protein
References
NaKagomi S., et al. (1999) Eur J Neurosci. 11:2160-2166.; Leiper J., et al. (2002) J Biol Chem. 276:40449-40456.
NCBI and Uniprot Product Information
NCBI GI #
NCBI GeneID
NCBI Accession #
NCBI GenBank Nucleotide #
Molecular Weight
33.5 kDa (308aa) confirmed by MALDI-TOF
NCBI Official Full Name
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1 isoform 1
NCBI Official Synonym Full Names
dimethylarginine dimethylaminohydrolase 1
NCBI Official Symbol
DDAH1
NCBI Official Synonym Symbols
DDAH; DDAHI; DDAH-1; HEL-S-16
NCBI Protein Information
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
UniProt Protein Name
N(G),N(G)-dimethylarginine dimethylaminohydrolase 1
Protein Family
UniProt Gene Name
DDAH1
UniProt Synonym Gene Names
DDAH; DDAH-1; Dimethylarginine dimethylaminohydrolase 1
NCBI Description
This gene belongs to the dimethylarginine dimethylaminohydrolase (DDAH) gene family. The encoded enzyme plays a role in nitric oxide generation by regulating cellular concentrations of methylarginines, which in turn inhibit nitric oxide synthase activity. [provided by RefSeq, Jul 2008]
Uniprot Description
Hydrolyzes N(G),N(G)-dimethyl-L-arginine (ADMA) and N(G)-monomethyl-L-arginine (MMA) which act as inhibitors of NOS. Has therefore a role in the regulation of nitric oxide generation.
Research Articles on DDAH1
Similar Products
Product Notes
The DDAH1 ddah1 (Catalog #AAA203757) is a Recombinant Protein produced from E Coli and is intended for research purposes only. The product is available for immediate purchase. The tag for this protein is His-tag. AAA Biotech's DDAH1 can be used in a range of immunoassay formats including, but not limited to, SDS-PAGE. Researchers should empirically determine the suitability of the DDAH1 ddah1 for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: MGSSHHHHHH SSGLVPRGSH MGSMAGLGHP AAFGRATHAV VRALPESLGQ HALRSAKGEE VDVARAERQH QLYVGVLGSK LGLQVVELPA DESLPDCVFV EDVAVVCEET ALITRPGAPS RRKEVDMMKE ALEKLQLNIV EMKDENATLD GGDVLFTGRE FFVGLSKRTN QRGAEILADT FKDYAVSTVP VADGLHLKSF CSMAGPNLIA IGSSESAQKA LKIMQQMSDH RYDKLTVPDD IAANCIYLNI PNKGHVLLHR TPEEYPESAK VYEKLKDHML IPVSMSELEK VDGLLTCCSV LINKKVDS. It is sometimes possible for the material contained within the vial of "DDAH1, Recombinant Protein" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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