Western Blot (WB)
(Western Blot: Sample: Human Lung Tissue.)
Rabbit anti-Human BH3 Interacting Domain Death Agonist (Bid) Polyclonal Antibody | anti-Bid antibody
Polyclonal Antibody to BH3 Interacting Domain Death Agonist (Bid)
MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD
Immunocytochemistry in formalin fixed cells: 1:100-500
Immunohistochemistry in formalin fixed frozen section: 1:100-500
Immunohistochemistry in paraffin section: 1:50-200
Enzyme-linked Immunosorbent Assay: 1:100-200
Western Blot (WB)
(Western Blot: Sample: Human Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Human Lung Tissue.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Bid, Human.)
Western Blot (WB)
(Western Blot: Sample: Recombinant Bid, Human.)
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
Immunohistochemistry (IHC)
(DAB staining on fromalin fixed paraffin-embedded Spleen tissue))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: Bid knockout Jurkat cell lysate; Predicted MW: 22kd Observed MW: 22kd Primary Ab: 2ug/ml Rabbit Anti-Human Bid Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
Knockout Validation
(Knockout Validation: Lane 1: Wild-type Jurkat cell lysate; Lane 2: Bid knockout Jurkat cell lysate; Predicted MW: 22kd Observed MW: 22kd Primary Ab: 2ug/ml Rabbit Anti-Human Bid Antibody Second Ab: 0.2ug/mL HRP-Linked Caprine Anti-Rabbit IgG Polyclonal Antibody (#MBS2086047))
NCBI and Uniprot Product Information
NCBI Description
This gene encodes a death agonist that heterodimerizes with either agonist BAX or antagonist BCL2. The encoded protein is a member of the BCL-2 family of cell death regulators. It is a mediator of mitochondrial damage induced by caspase-8 (CASP8); CASP8 cleaves this encoded protein, and the COOH-terminal part translocates to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced transcript variants have been found, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
Uniprot Description
BID: a pro-apoptotic member of the Bcl-2 superfamily. Targets intracellular membranes and contains a BH3 death domain. Heterodimerizes with either the pro-apoptotic protein BAX or the anti-apoptotic protein BCL2, antagonizing its protective effect. The activity of BID is regulated by Caspase 8-mediated cleavage, exposing the BH3 domain and significantly changing the surface charge and hydrophobicity, causing translocation to mitochondria where it triggers cytochrome c release. Multiple alternatively spliced variants have been found.
Protein type: Apoptosis; Mitochondrial
Chromosomal Location of Human Ortholog: 22q11.21
Cellular Component: cytosol; extracellular exosome; membrane; mitochondrial outer membrane; mitochondrion
Molecular Function: death receptor binding; protein binding; protein heterodimerization activity; ubiquitin protein ligase binding
Biological Process: activation of cysteine-type endopeptidase activity involved in apoptotic process; apoptotic mitochondrial changes; establishment of protein localization to membrane; induction of apoptosis via death domain receptors; negative regulation of intrinsic apoptotic signaling pathway in response to DNA damage; neuron apoptosis; organelle ATP synthesis coupled electron transport; positive regulation of apoptosis; positive regulation of extrinsic apoptotic signaling pathway; positive regulation of fibroblast apoptotic process; positive regulation of intrinsic apoptotic signaling pathway; positive regulation of mitochondrial membrane potential; positive regulation of mitochondrial outer membrane permeabilization involved in apoptotic signaling pathway; positive regulation of protein homooligomerization; positive regulation of protein insertion into mitochondrial membrane involved in apoptotic signaling pathway; positive regulation of protein oligomerization; positive regulation of release of cytochrome c from mitochondria; protein homooligomerization; protein targeting to mitochondrion; regulation of apoptosis; regulation of cell proliferation; regulation of G1/S transition of mitotic cell cycle; release of cytochrome c from mitochondria; signal transduction in response to DNA damage
Research Articles on Bid
Similar Products
Product Notes
The Bid bid (Catalog #AAA2002257) is an Antibody produced from Rabbit and is intended for research purposes only. The product is available for immediate purchase. The Polyclonal Antibody to BH3 Interacting Domain Death Agonist (Bid) reacts with Human and may cross-react with other species as described in the data sheet. AAA Biotech's BH3 Interacting Domain Death Agonist (Bid) can be used in a range of immunoassay formats including, but not limited to, Immunocytochemistry (ICC), Immunohistochemistry (IHC) - Formalin/Paraffin, ELISA (EIA), Western Blot (WB). Western blotting: 1:100-400 Immunocytochemistry in formalin fixed cells: 1:100-500 Immunohistochemistry in formalin fixed frozen section: 1:100-500 Immunohistochemistry in paraffin section: 1:50-200 Enzyme-linked Immunosorbent Assay: 1:100-200. Researchers should empirically determine the suitability of the Bid bid for an application not listed in the data sheet. Researchers commonly develop new applications and it is an integral, important part of the investigative research process. The amino acid sequence is listed below: Antigen: The target protein is fused with two N-terminal Tags, His-tag and its sequence is listed below. MGSSHHHHHH SSGLVPRGSH MASMTGGQQM GRGSEF- MDCEVNNGSS LRDECITNLL VFGFLQSCSD NSFRRELDAL GHELPVLAPQ WEGYDELQTD GNRSSHSRLG RIEADSESQE DIIRNIARHL AQVGDSMDRS IPPGLVNGLA LQLRNTSRSE EDRNRDLATA LEQLLQAYPR DMEKEKTMLV LALLLAKKVA SHTPSLLRDV FHTTVNFINQ NLRTYVRSLA RNGMD. It is sometimes possible for the material contained within the vial of "BH3 Interacting Domain Death Agonist (Bid), Polyclonal Antibody" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
If you are ready to order, navigate to Shopping Cart and get ready to checkout.
