m7GpppN-mRNA hydrolase Recombinant Protein | DCP2 recombinant protein

Recombinant Human m7GpppN-mRNA hydrolase

Cat. Num. AAA9422792

• Gene Names: DCP2; NUDT20
• Reactivity: Human
• Purity: Greater than 90% as determined by SDS-PAGE.
• Synonyms: m7GpppN-mRNA hydrolase; Recombinant Human m7GpppN-mRNA hydrolase; Nucleoside diphosphate-linked moiety X motif 20; Nudix motif 20mRNA-decapping enzyme 2; hDpc; DCP2 recombinant protein

• Host: Yeast
• Reactivity: Human
• Purity/Purification: Greater than 90% as determined by SDS-PAGE.
• Form/Format: 20mM Tris-HCl based buffer, pH8.0
• Sequence Positions: Isoform 2, 1-385aa

• Tag Info: His-tag
• Preparation and Storage: Store at -20 degree C, for extended storage, conserve at -20 degree C or -80 degree C. Repeated freezing and thawing is not recommended. Store working aliquots at 4 degree C for up to one week.

Related Product Information for DCP2 recombinant protein

Decapping metalloenzyme that catalyzes the cleavage of the cap structure on mRNAs. Roves the 7-methyl guanine cap structure from mRNA molecules, yielding a 5'-phosphorylated mRNA fragment and 7m-GDP. Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Has higher activity towards mRNAs that lack a poly (A) tail. Has no activity towards a cap structure lacking an RNA moiety

NCBI and Uniprot Product Information

• NCBI GI #: 334688848
• NCBI GeneID: 167227
• NCBI Accession #: NP_001229306.1
• NCBI GenBank Nucleotide #: NM_001242377.1
• UniProt Accession #: Q8IU60; Q6P2D4; Q7Z5W5; Q8NBG5; C9J778
• Molecular Weight: 46.4kD
• NCBI Official Full Name: m7GpppN-mRNA hydrolase isoform 2
• NCBI Official Synonym Full Names: decapping mRNA 2
• NCBI Official Symbol: DCP2
• NCBI Official Synonym Symbols: NUDT20
• NCBI Protein Information: m7GpppN-mRNA hydrolase
• UniProt Protein Name: m7GpppN-mRNA hydrolase
• Protein Family: mRNA-decapping enzyme
• UniProt Gene Name: DCP2
• UniProt Synonym Gene Names: NUDT20; Nudix motif 20; hDpc

NCBI Description

The protein encoded by this gene is a key component of an mRNA-decapping complex required for degradation of mRNAs, both in normal mRNA turnover, and in nonsense-mediated mRNA decay (NMD). It removes the 7-methyl guanine cap structure from mRNA, prior to its degradation from the 5' end. Alternatively spliced transcript variants encoding different isoforms have been noted for this gene.[provided by RefSeq, Jun 2011]

Uniprot Description

DCP2: Necessary for the degradation of mRNAs, both in normal mRNA turnover and in nonsense-mediated mRNA decay. Plays a role in replication-dependent histone mRNA degradation. Removes the 7- methyl guanine cap structure from mRNA molecules, yielding a 5'- phosphorylated mRNA fragment and 7m-GDP. Has higher activity towards mRNAs that lack a poly(A) tail. Has no activity towards a cap structure lacking a RNA moiety. Belongs to the Nudix hydrolase family. DCP2 subfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: EC 3.6.1.62; Hydrolase; RNA processing

Chromosomal Location of Human Ortholog: 5q22.2

Cellular Component: cell junction; cytosol; nucleoplasm

Molecular Function: 5'-3' exoribonuclease activity; exoribonuclease activity, producing 5'-phosphomonoesters; m7G(5')pppN diphosphatase activity; protein binding

Biological Process: deadenylation-dependent decapping; mRNA catabolic process; negative regulation of telomere maintenance via telomerase; regulation of mRNA stability