Human Decorin ELISA Kit | DCN elisa kit

Human Decorin ELISA Kit

Cat. Num. AAA762622

• Gene Names: DCN; CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• Reactivity: Human
• Synonyms: Decorin; Human Decorin ELISA Kit; DCN (Decorin)/DSPG2/PG-II/PGS2/SLRR1B/Bone proteoglycan II/CSCD/PG40/PGII/PG-S2; DCN elisa kit

• Reactivity: Human
• Sequence Length: 359

• Samples: Serum, plasma and other biological fluids.
• Assay Type: Sandwich ELISA, Double Antibody
• Detection Range: 62.5-4000pg/ml
• Sensitivity: 0.75ng/ml
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data

Typical Standard Curve

Related Product Information for DCN elisa kit

Principle of the Assay||This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Anti-human DCNantibody was pre-coated onto 96-well plates. And the biotin conjugated anti-human DCN antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and wash with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the human DCN amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration ofhuman DCNcan be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 19743846
• NCBI GeneID: 1634
• NCBI Accession #: NP_598010.1
• NCBI GenBank Nucleotide #: NM_133503.3
• Molecular Weight: 8,266 Da
• NCBI Official Full Name: decorin isoform a preproprotein
• NCBI Official Synonym Full Names: decorin
• NCBI Official Symbol: DCN
• NCBI Official Synonym Symbols: CSCD; PG40; PGII; PGS2; DSPG2; SLRR1B
• NCBI Protein Information: decorin
• UniProt Protein Name: Decorin
• Protein Family: Decorin
• UniProt Gene Name: DCN
• UniProt Synonym Gene Names: SLRR1B
• UniProt Entry Name: PGS2_HUMAN

NCBI Description

This gene encodes a member of the small leucine-rich proteoglycan family of proteins. Alternative splicing results in multiple transcript variants, at least one of which encodes a preproprotein that is proteolytically processed to generate the mature protein. This protein plays a role in collagen fibril assembly. Binding of this protein to multiple cell surface receptors mediates its role in tumor suppression, including a stimulatory effect on autophagy and inflammation and an inhibitory effect on angiogenesis and tumorigenesis. This gene and the related gene biglycan are thought to be the result of a gene duplication. Mutations in this gene are associated with congenital stromal corneal dystrophy in human patients. [provided by RefSeq, Nov 2015]

Uniprot Description

DCN: May affect the rate of fibrils formation. Defects in DCN are the cause of congenital stromal corneal dystrophy (CSCD). Corneal dystrophies are inherited, bilateral, primary alterations of the cornea that are not associated with prior inflammation or secondary to systemic disease. Most show autosomal dominant inheritance. Belongs to the small leucine-rich proteoglycan (SLRP) family. SLRP class I subfamily. 5 isoforms of the human protein are produced by alternative splicing.

Protein type: Secreted, signal peptide; Motility/polarity/chemotaxis; Secreted; Extracellular matrix

Chromosomal Location of Human Ortholog: 12q21.33

Cellular Component: collagen type VI; cytoplasm; extracellular matrix; extracellular region; extracellular space; Golgi lumen; lysosomal lumen

Molecular Function: collagen binding; extracellular matrix binding; glycosaminoglycan binding; protein kinase inhibitor activity; protein N-terminus binding

Biological Process: aging; carbohydrate metabolic process; chondroitin sulfate biosynthetic process; chondroitin sulfate catabolic process; chondroitin sulfate metabolic process; cytokine and chemokine mediated signaling pathway; dermatan sulfate biosynthetic process; extracellular matrix disassembly; extracellular matrix organization and biogenesis; glycosaminoglycan metabolic process; kidney development; negative regulation of angiogenesis; negative regulation of JAK-STAT cascade; negative regulation of protein kinase activity; organ morphogenesis; peptide cross-linking via chondroitin 4-sulfate glycosaminoglycan; placenta development; positive regulation of autophagy; positive regulation of macroautophagy; positive regulation of mitochondrial depolarization; positive regulation of phosphoinositide 3-kinase cascade; positive regulation of transcription from RNA polymerase II promoter; response to lipopolysaccharide; response to mechanical stimulus; skeletal muscle development; wound healing

Disease: Corneal Dystrophy, Congenital Stromal