Rabbit anti-Human, Mouse HUNK Polyclonal Antibody | anti-HUNK antibody

HUNK, CT (Hormonally Upregulated Neu Tumor-Associated Kinase, B19, MAKV, Serine/threonine-protein Kinase MAK-V) (APC)

Cat. Num. AAA6481240

• Reactivity: Human, Mouse
• Applications: Immunohistochemistry, Western Blot
• Purity: Purified by Protein G Affinity Chromatography.
• Synonyms: HUNK; Polyclonal Antibody; CT (Hormonally Upregulated Neu Tumor-Associated Kinase; B19; MAKV; Serine/threonine-protein Kinase MAK-V) (APC); Hormonally Up-regulated Neu-associated Kinase; EC=2.7.11.1; anti-HUNK antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Isotype: IgG
• Specificity: Recognizes human HUNK. Species Crossreactivity: mouse.
• Purity/Purification: Purified by Protein G Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with Allophycocyanin (APC).

• Applicable Applications for anti-HUNK antibody: Immunohistochemistry (IHC), FLISA, Western Blot (WB)
• Application Notes: IHC: 1:50-1:100; FLISA: 1:1,000; WB: 1:100-1:500; Applications are based on unconjugated antibody.
• Immunogen: Synthetic peptide selected from the C-terminal region of human HUNK (KLH).
• Conjugate: APC
• Note: Preservative Free
• Special Handling: Light sensitive
• Preparation and Storage: May be stored at 4 degree C for short-term only. Aliquot to avoid repeated freezing and thawing. Do not freeze APC conjugates. Light sensitive. Aliquots are stable for at least 6 months. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(The anti-HUNK Pab is used in Western blot to detect HUNK in NIH/3T3 cell lysate.)

Immunohistochemistry (IHC)

(Formalin-fixed and paraffin-embedded human cancer tissue reacted with the primary antibody, which was peroxidase-conjugated to the secondary antibody, followed by AEC staining. This data demonstrates the use of this antibody for immunohistochemistry; clinical relevance has not been evaluated. BC = breast carcinoma; HC = hepatocarcinoma.)

Product Categories/Family for anti-HUNK antibody

Antibodies; Protein Kinases

References

1. Gardner, H.P., et al., Genomics 63(1):46-59 (2000).

NCBI and Uniprot Product Information

• NCBI GI #: 7657214
• NCBI GeneID: 30811
• NCBI Accession #: NP_055401.1
• NCBI GenBank Nucleotide #: NM_014586.1
• UniProt Accession #: P57058
• Molecular Weight: 79,686 Da
• NCBI Official Full Name: hormonally up-regulated neu tumor-associated kinase
• NCBI Official Synonym Full Names: hormonally up-regulated Neu-associated kinase
• NCBI Official Symbol: HUNK
• NCBI Protein Information: hormonally up-regulated neu tumor-associated kinase; B19; serine/threonine protein kinase MAK-V; serine/threonine-protein kinase MAK-V; hormonally upregulated Neu-associated kinase; hormonally upregulated neu tumor-associated kinase
• UniProt Protein Name: Hormonally up-regulated neu tumor-associated kinase
• Protein Family: Hormonally up-regulated neu tumor-associated kinase
• UniProt Gene Name: HUNK
• UniProt Synonym Gene Names: MAKV
• UniProt Entry Name: HUNK_HUMAN

Uniprot Description

HUNK: a protein kinase of the CAMKL family. Is developmentally regulated in a tissue-specific fashion. May contribute to changes in the mammary gland that occur during pregnancy in response to ovarian hormones. The active enzyme is localized in the nucleocytoplasm and the centrosome.

Protein type: Protein kinase, CAMK; Protein kinase, Ser/Thr (non-receptor); Kinase, protein; EC 2.7.11.1; CAMK group; CAMKL family; HUNK subfamily

Chromosomal Location of Human Ortholog: 21q22.1

Molecular Function: protein serine/threonine kinase activity; ATP binding

Biological Process: multicellular organismal development; signal transduction; protein amino acid phosphorylation