Rat cytochrome P450, family 2, subfamily E, polypeptide 1 ELISA Kit | CYP2E1 elisa kit

Rat cytochrome P450 2E1, CYP2E1 ELISA Kit

Cat. Num. AAA702246

• Gene Names: LOC100342572; CYP2E; CYP2E1; CYPIIE1
• Reactivity: Rat
• Synonyms: cytochrome P450; family 2; subfamily E; polypeptide 1; Rat cytochrome P450 2E1; CYP2E1 ELISA Kit; CPE1; CYP2E; P450-J; P450C2E; cytochrome P450 2E1; subfamily IIE (ethanol-inducible); flavoprotein-linked monooxygenase; microsomal monooxygenase; xenobiotic monooxygenas; CYP2E1 elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of Rat CYP2E1. No significant cross-reactivity or interference between Rat CYP2E1 and analogues was observed.
• Sequence Length: 493

• Samples: Serum, plasma, tissue homogenates
• Detection Range: 0.156 ng/ml-10 ng/ml
• Sensitivity: 0.039 ng/ml
• Intra-assay Precision: Intra-assay Precision (Precision within an assay): CV% is less than 8%; Three samples of known concentration were tested twenty times on one plate to assess.; Inter-assay Precision (Precision between assays): CV% is less than 10%; Three samples of known concentration were tested in twenty assays to assess.
• Detection Wavelength: 450 nm
• Sample Volume: 50-100ul
• Preparation and Storage: Unopened test kits should be stored at 2 to 8 degree C upon receipt. Please refer to pdf manual for further storage instructions.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CYP2E1 elisa kit

Introduction: We are constantly exposed to many potentially toxic chemicals. Most require metabolic activation to species responsible for cell injury. Although cytochrome P450 2E1 is only one of many different forms of cytochrome P450 that catalyze these reactions, it has an important role in human health as a result of being readily induced by acute and chronic alcohol ingestion. The enzyme efficiently catalyzes the low Km metabolism of compounds commonly used as solvents in industry and at home as well as components found in cigarette smoke, many of which are established carcinogens and hepatotoxins. As a result, there is the potential for increased risk to low level exposure to such chemicals while cytochrome P450 2E1 is induced. Many substrates have been identified for cytochrome P450 2E1. Of the 52 substrates for the enzyme identified in this review, the demethylation of N,N-dimethylnitrosamine and the hydroxylation of p-nitrophenol and chlorzoxazone are the most effective for monitoring the level of this enzyme. In addition to oxidative reactions, cytochrome P450 2E1 is also an efficient catalyst of reductive reactions. CCl4-induced hepatotoxicity is one of the best- documented cases for the participation of cytochrome P450 2E1 in a toxicologically important reductive reaction. The reduction of oxygen to superoxide and peroxide are also important reductive reactions of the enzyme and could be important in lipid peroxidation. However, the role of this reaction in vivo remains controversial.

Principle of the Assay: The microtiter plate provided in this kit has been pre-coated with an antibody specific to CYP2E1. Standards or samples are then added to the 3 appropriate microtiter plate wells with a biotin-conjugated polyclonal antibody preparation specific for CYP2E1. and Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. Then a TMB (3,3',5,5' tetramethyl-benzidine) substrate solution is added to each well. Only those wells that contain CYP2E1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of a sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450 nm +/- 2 nm. The concentration of CYP2E1. in the samples is then determined by comparing the O.D. of the samples to the standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 291404953
• NCBI GeneID: 100342572
• NCBI Accession #: XP_002718818.1
• NCBI GenBank Nucleotide #: XM_002718772.2
• UniProt Accession #: P08682
• Molecular Weight: 56,945 Da
• NCBI Official Full Name: cytochrome P450 2E1
• NCBI Official Symbol: LOC100342572
• NCBI Official Synonym Symbols: CYP2E; CYP2E1; CYPIIE1
• NCBI Protein Information: cytochrome P450 2E1; Cytochrome P450-ALC; Cytochrome P450 LM3A; Cytochrome P450 isozyme 3A; 4-nitrophenol 2-hydroxylase
• UniProt Protein Name: Cytochrome P450 2E1
• Protein Family: Cytochrome
• UniProt Gene Name: CYP2E1
• UniProt Synonym Gene Names: CYP2E; Cytochrome P450 LM3A
• UniProt Entry Name: CP2E1_RABIT

Uniprot Description

Function: Metabolizes several precarcinogens, drugs, and solvents to reactive metabolites

Catalytic activity: 4-nitrophenol + NADPH + O2 = 4-nitrocatechol + NADP+ + H2O.

Cofactor: Heme group