BCA-1 (CXCL13) Recombinant Protein | BCA-1 recombinant protein

Human BCA-1 (CXCL13) Recombinant

Cat. Num. AAA553043

• Purity: >98%, as determined by SDS-PAGE and HPLC
• Synonyms: BCA-1 (CXCL13); Human BCA-1 (CXCL13) Recombinant; Human BCA1 (CXCL13) Recombinant; bca-1; bca1; cxcl13; cxcl-13; BCA-1 recombinant protein

• Host: E Coli
• Purity/Purification: >98%, as determined by SDS-PAGE and HPLC
• Form/Format: Recombinant BCA-1 was lyophilized from a 0.2 um filtered 20mM PB,100mM NaCl solution pH 7.5.
• Sequence: MKFISTSLLL MLLVSSLSPV QGVLEVYYTS LRCRCVQESS VFIPRRFIDR IQILPRGNGC PRKEIIVWKK NKSIVCVDPQ AEWIQRMMEV LRKRSSSTLP VPVFKRKIP
• Sequence Length: 109

• Host Note: Optimized DNA sequence encoding Human BCA-1 (CXCL13) mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Human CXCL13, generated by the proteolytic removal of the signal peptide; and propeptide and has a calculated molecular mass of approximately 10 kDa.; ; Recombinant CXCL13 is a disulfide-linked monomeric protein consisting of 88 amino acid residue subunits and migrates as an approximately 10 kDa protein under non-reducing conditions and reducing conditions in SDS-PAGE.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for BCA-1 recombinant protein

The homeostatic chemokine CXCL13 (also called B cell- attracting chemokine 1 [BCA- 1] or B-lymphocyte chemoattractant [BLC]) is constitutively expressed in secondary lymphoid tissue and initiates lymphoid neogenesis when expressed aberrantly in mice. CXCL13 has also been detected in chronic inflammation associated with human lymphoid neogenesis, suggesting a pathogenic role. Follicular dendritic cells (FDCs) are generally considered to be the major source of CXCL13 both in normal and aberrant lymphoid tissue.CXCL13 has been the exclusive ligand for CXCR5, which initiates chemotaxis toward an increasing gradient in vitro and to B cell zones in lymph nodes that express CXCL13. More recently, CXCR5 expression has also been shown in DN T cells in mice, a subset of CD4 follicular Th cells, and some tonsillar CD4+CD25+ Tregs in humans

Product Categories/Family for BCA-1 recombinant protein

Cytokines

References

• serum blc/CXCL13 concentrations and renal expression of CXCL13/cxcr5 in patients with systemic lupus erythematosus and lupus nephritis .J Rheumatol, Jan 2010; 37: 45 - 52.
• increased cerebrospinal fluid concentrations of the chemokine CXCL13 in active ms .Neurology, Dec 2009; 73: 2003 - 2010.
• the chemokine CXCL13 in acute neuroborreliosis .J Neurol Psychiatry, Dec 2009; 10.1136/jnnp.2009.195438.
• b-cell-attracting chemokine CXCL13 as a marker of disease activity and renal involvement in systemic lupus erythematosus (sle) .Nephrol. Dial. Transplant., Dec 2009; 24: 3708 - 3712.
• serum levels of CXCL13 are elevated in active multiple sclerosis .Multiple Sclerosis, Nov 2009; 15: 1271 - 1279.