CXCL12 recombinant protein

Recombinant Mouse CXCL12 Protein

Cat. Num. AAA8248904

• Gene Names: Cxcl12; Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• Reactivity: Mouse
• Applications: ELISA, Western Blot, SDS-Page
• Purity: Greater than 95% as determined by reducing SDS-PAGE.
• Synonyms: CXCL12; Recombinant Mouse CXCL12 Protein; SDF1; SDF1A; SDF1B; Stromal cell-derived factor 1; SDF-1; hSDF-1; C-X-C motif chemokine 12; Intercrine reduced in hepatomas; IRH; hIRH; Pre-B cell growth-stimulating factor; PBSF; CXCL12 recombinant protein

• Host: E Coli
• Reactivity: Mouse
• Purity/Purification: Greater than 95% as determined by reducing SDS-PAGE.
• Form/Format: Lyophilized from a 0.2 muM filtered solution of PBS, pH 7.4.
• Sequence: MKPVSLSYRC PCRFFESHIA RANVKHLKIL NTPNCALQIV ARLKNNNRQV CIDPKLKWIQ EYLEKALNK
• Sequence Length: 93

• Applicable Applications for CXCL12 recombinant protein: ELISA (EIA),Western Blot (WB), SDS-PAGE, Mass Spectrometry (MS)
• Endotoxin: Less than 0.1 ng/mug (1 IEU/mug) as determined by LAL test.
• Directions for Use: Always centrifuge tubes before opening. Do not mix by vortex or pipetting. It is not recommended to reconstitute to a concentration less than 100 mug/ml. Dissolve the lyophilized protein in 1X PBS. Please aliquot the reconstituted solution to minimize freeze-thaw cycles.
• Preparation and Storage: Lyophilized protein should be stored at -20 degree C, though stable at room temperature for 3 weeks. Reconstituted protein solution can be stored at 2-8 degree C for 2-7 days. Aliquots of reconstituted samples are stable at -20 degree C for 3 months.

Related Product Information for CXCL12 recombinant protein

Recombinant Mouse CXCL12 Protein is produced by our E Coli expression system and the target gene encoding Lys22-Lys89 is expressed.

NCBI and Uniprot Product Information

• NCBI GI #: 7305465
• NCBI GeneID: 20315
• NCBI Accession #: NP_038683.1
• NCBI GenBank Nucleotide #: NM_013655.4
• UniProt Accession #: P40224; Q4FJL5; Q543V6
• Molecular Weight: 10,032 Da
• NCBI Official Full Name: stromal cell-derived factor 1 isoform beta
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 12
• NCBI Official Symbol: Cxcl12
• NCBI Official Synonym Symbols: Pbsf; Sdf1; Tlsf; Tpar1; Scyb12
• NCBI Protein Information: stromal cell-derived factor 1
• UniProt Protein Name: Stromal cell-derived factor 1
• Protein Family: Stromal cell-derived factor
• UniProt Gene Name: Cxcl12
• UniProt Synonym Gene Names: Sdf1; SDF-1; TPAR1; PBSF; TLSF
• UniProt Entry Name: SDF1_MOUSE

NCBI Description

This gene encodes a member of the alpha chemokine protein family. The encoded protein is secreted and functions as the ligand for the G-protein coupled receptor, chemokine (C-X-C motif) receptor 4. The encoded protein plays a role in many diverse cellular functions, including embryogenesis, immune surveillance, inflammation response, tissue homeostasis, and tumor growth and metastasis. Alternative splicing results in multiple transcript variants. [provided by RefSeq, May 2013]

Uniprot Description

Chemoattractant active on T-lymphocytes, monocytes, but not neutrophils. Activates the C-X-C chemokine receptor CXCR4 to induce a rapid and transient rise in the level of intracellular calcium ions and chemotaxis. Also binds to atypical chemokine receptor ACKR3, which activates the beta-arrestin pathway and acts as a scavenger receptor for SDF-1. Acts as a positive regulator of monocyte migration and a negative regulator of monocyte adhesion via the LYN kinase. Stimulates migration of monocytes and T-lymphocytes through its receptors, CXCR4 and ACKR3, and decreases monocyte adherence to surfaces coated with ICAM-1, a ligand for beta-2 integrins. SDF1A/CXCR4 signaling axis inhibits beta-2 integrin LFA-1 mediated adhesion of monocytes to ICAM-1 through LYN kinase. Plays a protective role after myocardial infarction. Induces down-regulation and internalization of ACKR3 expressed in various cells (). Has several critical functions during embryonic development; required for B-cell lymphopoiesis, myelopoiesis in bone marrow and heart ventricular septum formation.