Goat NCF1 / p47phox / p47 phox Polyclonal Antibody | anti-NCF1 antibody

Goat Polyclonal to Human NCF1 / p47phox / p47 phox

Cat. Num. AAA241996

• Gene Names: NCF1; NCF1A; NOXO2; p47phox; SH3PXD1A
• Reactivity: Bovine, Human, Monkey
• Applications: Immunohistochemistry, Western Blot, ELISA
• Purity: Immunoaffinity Purified
• Synonyms: NCF1 / p47phox / p47 phox; Polyclonal Antibody; Goat Polyclonal to Human NCF1 / p47phox / p47 phox; Anti-NCF1 / p47phox / p47 phox Antibody (C-Terminus) IHC-plus; NCF1; NCF1A; Neutrophil cytosolic factor 1; NCF-47K; Nox organizer 2; NOXO2; p47-phox; p47phox; SH3PXD1A; NADPH oxidase organizer 2; NCF-1; Neutrophil cytosol factor 1; Nox-organizing protein 2; Human NCF1; p47 phox; anti-NCF1 antibody

• Host: Goat
• Reactivity: Bovine, Human, Monkey
• Clonality: Polyclonal
• Specificity: Human NCF1 / p47phox.
• Purity/Purification: Immunoaffinity Purified
• Form/Format: Tris-buffered saline, pH 7.3, 0.5% BSA, 0.02% sodium azide
• Concentration: 0.5 mg/ml (varies by lot)
• Sequence Length: 390

• Applicable Applications for anti-NCF1 antibody: Immunohistochemistry (IHC - Paraffin), Western Blot (WB), ELISA (EIA)
• Application Notes: ELISA, IHC-P (5 ug/ml), WB (0.2 - 2 ug/ml); Usage: Immunohistochemistry: was validated for use in immunohistochemistry on a panel of 21 formalin-fixed, paraffin-embedded (FFPE) human tissues after heat induced antigen retrieval in pH 6.0 citrate buffer. After incubation with the primary anti...
• Target Species: Human
• Immunogen Description: Synthetic peptide C-SESTKRKLASAV from the C-terminus of human NCF1 / p47phox (NP_000256.3). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%); Mouse, Rat (85%).
• Immunogen Type: Synthetic peptide
• Immunogen: NCF1 / p47phox / p47 phox antibody was raised against synthetic peptide C-SESTKRKLASAV from the C-terminus of human NCF1 / p47phox (NP_000256.3). Percent identity by BLAST analysis: Human, Monkey, Marmoset, Dolphin, Elephant, Panda, Bovine (100%); Mouse, Rat (85%).
• Antigen Modification: C-Terminus
• Preparation and Storage: Store at -20 degree C. Minimize freezing and thawing.

Immunohistochemistry (IHC)

(Anti-p47phox antibody IHC of human colon, neutrophils. Immunohistochemistry of formalin-fixed, paraffin-embedded tissue after heat-induced antigen retrieval. Antibody concentration 5 ug/ml.)

Western Blot (WB)

(Staining (0.2 ug/ml) of U937 lysate (RIPA buffer, 30 ug total protein per lane). Primary incubated for 1 hour. Detected by chemiluminescence.)

Related Product Information for anti-NCF1 antibody

NCF1 encodes neutrophil cytosolic factor 1, the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. This oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

NCBI and Uniprot Product Information

• NCBI GI #: 115298672
• NCBI GeneID: 653361
• NCBI Accession #: NP_000256.3
• NCBI GenBank Nucleotide #: NM_000265.4
• UniProt Accession #: P14598; O43842; Q2PP07; Q53FR5; Q9BU90; Q9BXI7; Q9BXI8; Q9UDV9; Q9UMU2; A6NEH2; A8K7S9
• Molecular Weight: 30,781 Da
• NCBI Official Full Name: neutrophil cytosol factor 1
• NCBI Official Synonym Full Names: neutrophil cytosolic factor 1
• NCBI Official Symbol: NCF1
• NCBI Official Synonym Symbols: NCF1A; NOXO2; p47phox; SH3PXD1A
• NCBI Protein Information: neutrophil cytosol factor 1; NCF-1; NCF-47K; p47-phox; nox organizer 2; nox-organizing protein 2; NADPH oxidase organizer 2; 47 kDa neutrophil oxidase factor; neutrophil NADPH oxidase factor 1; SH3 and PX domain-containing protein 1A; 47 kDa autosomal chronic granulomatous disease protein; neutrophil cytosolic factor 1, (chronic granulomatous disease, autosomal 1)
• UniProt Protein Name: Neutrophil cytosol factor 1
• Protein Family: Neutrophil cytosol factor
• UniProt Gene Name: NCF1
• UniProt Synonym Gene Names: NOXO2; SH3PXD1A; NCF-1
• UniProt Entry Name: NCF1_HUMAN

NCBI Description

The protein encoded by this gene is a 47 kDa cytosolic subunit of neutrophil NADPH oxidase. This oxidase is a multicomponent enzyme that is activated to produce superoxide anion. Mutations in this gene have been associated with chronic granulomatous disease. [provided by RefSeq, Jul 2008]

Uniprot Description

p47phox: the 47-kilodalton cytosolic subunit of the multi-protein complex known as NADPH oxidase found in neutrophils. The holo-oxidase produces a burst of superoxide which is delivered to the lumen of the neutrophil phagosome. Contains 2 SH2 domains. Mutations in NCF1, as well as in other NADPH oxidase subunits, can result in chronic granulomatous disease.

Protein type: Oxidoreductase; EC 1.-.-.-

Chromosomal Location of Human Ortholog: 7q11.23

Cellular Component: Golgi apparatus; extrinsic to membrane; rough endoplasmic reticulum; cell soma; dendrite; cytosol; NADPH oxidase complex

Molecular Function: protein binding; electron carrier activity; superoxide-generating NADPH oxidase activity; phosphatidylinositol-3,4-bisphosphate binding; SH3 domain binding; phosphoinositide binding

Biological Process: respiratory burst; respiratory burst during defense response; interaction with host; apoptosis; superoxide metabolic process; antigen processing and presentation of exogenous peptide antigen via MHC class I, TAP-dependent; leukotriene metabolic process; cell proliferation; antigen processing and presentation of peptide antigen via MHC class I; negative regulation of smooth muscle contraction; antigen processing and presentation of exogenous peptide antigen via MHC class I; innate immune response; cellular defense response; response to yeast; inflammatory response; vascular endothelial growth factor receptor signaling pathway; protein targeting to membrane; superoxide release; hydrogen peroxide biosynthetic process

Disease: Granulomatous Disease, Chronic, Autosomal Recessive, Cytochrome B-positive, Type I