CXCL1 recombinant protein

RECOMBINANT MOUSE CXCL1

Cat. Num. AAA232359

• Gene Names: Cxcl1; KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• Applications: ELISA, Functional Assay, Western Blot
• Purity: >98% by SDS page and HPLC analysis
• Synonyms: CXCL1; RECOMBINANT MOUSE CXCL1; CXCL1 recombinant protein

• Purity/Purification: >98% by SDS page and HPLC analysis
• Form/Format: Purified Recombinant Protein - lyophilised
• Concentration: Approximate Protein Concentration: 1.0mg/ml after reconstitution (varies by lot)
• Sequence Length: 96

• Applicable Applications for CXCL1 recombinant protein: ELISA (EIA), Functional Assays (FN), Western Blot (WB)
• Application Notes: ELISA: Minimum Dilution: 0.2; Maximum Dilution: 0.4ng/well;; Functional Assays: Minimum Dilution: 0.01; Maximum Dilution: 0.10ug/ml;; Western Blotting: Minimum Dilution: 1.5; Maximum Dilution: 3.0ng/lane
• Reconstitution: Reconstitute with 20ul distilled water. Care should be taken during reconstitution as the protein may appear as a film at the bottom of the vial. MyBioSource recommends that the vial is gently mixed after reconstitution. ; Preparation
• Target Species: Mouse
• Preparation and Storage: Prior to reconstitution store at 4 degree C. Following reconstitution store at -20 degree C. This product should be stored undiluted. Storage in frost-free freezers is not recommended. Avoid repeated freezing and thawing as this may denature the protein. Should this product contain a precipitate we recommend microcentrifugation before use.; Shelf Life: 3 months from date of reconstitution.

Related Product Information for CXCL1 recombinant protein

CXCL1, also known as GRO1, is a 7.8kD chemokine which is involved in neutrophil chemotaxis and activation and is expressed in a range of cells including monocytes, macrophages and vascular endothelial cells.

NCBI and Uniprot Product Information

• NCBI GI #: 6680109
• NCBI GeneID: 14825
• NCBI Accession #: NP_032202.1
• NCBI GenBank Nucleotide #: NM_008176.3
• UniProt Accession #: P12850
• Molecular Weight: Protein Molecular Weight: 7.8kD (72 amino acid sequence)
• NCBI Official Full Name: growth-regulated alpha protein
• NCBI Official Synonym Full Names: chemokine (C-X-C motif) ligand 1
• NCBI Official Symbol: Cxcl1
• NCBI Official Synonym Symbols: KC; Fsp; N51; gro; Gro1; Mgsa; Scyb1
• NCBI Protein Information: growth-regulated alpha protein; C-X-C motif chemokine 1; GRO1 oncogene; KC/GR)-alpha; KC/GRO-alpha; alpha-chemokine; platelet-derived growth factor-inducible protein KC; secretory protein N51
• UniProt Protein Name: Growth-regulated alpha protein
• Protein Family: Growth-regulated alpha protein
• UniProt Gene Name: Cxcl1
• UniProt Synonym Gene Names: Gro; Gro1; Mgsa; Scyb1; HSF
• UniProt Entry Name: GROA_MOUSE

NCBI Description

This gene encodes a protein that is a member of the CXC subfamily of chemokines. Chemokines, which recruit and activate leukocytes, are classified by function (inflammatory or homeostatic) or by structure. This secretory protein is proposed to bind the G-protein coupled receptor chemokine (C-X-C motif) receptor 2 to recruit neutrophils. In mouse, deficiency of this gene is associated with colitis and with defects in immune cell recruitment to the lung. [provided by RefSeq, Apr 2013]

Uniprot Description

CXCL2: Produced by activated monocytes and neutrophils and expressed at sites of inflammation. Hematoregulatory chemokine, which, in vitro, suppresses hematopoietic progenitor cell proliferation. GRO-beta(5-73) shows a highly enhanced hematopoietic activity. Belongs to the intercrine alpha (chemokine CxC) family.

Protein type: Secreted, signal peptide; Secreted; Motility/polarity/chemotaxis

Cellular Component: extracellular space; cell; extracellular region; cytosol

Molecular Function: growth factor activity; chemokine activity; CXCR chemokine receptor binding; cytokine activity

Biological Process: G-protein coupled receptor protein signaling pathway; neutrophil chemotaxis; elevation of cytosolic calcium ion concentration; positive regulation of superoxide release; response to molecule of bacterial origin; positive regulation of potassium ion transport; positive regulation of leukocyte chemotaxis; immune response; response to lipopolysaccharide; inflammatory response; acute inflammatory response; regulation of cell proliferation