Rabbit anti-Human Fractalkine Polyclonal Antibody | anti-CX3CL1 antibody

Anti-Fractalkine Antibody, Rabbit Polyclonal

Cat. Num. AAA8103418

• Gene Names: CX3CL1; NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• Reactivity: Human
• Applications: ELISA, Immunohistochemistry
• Purity: Protein A & Antigen Affinity
• Synonyms: Fractalkine; Polyclonal Antibody; Anti-Fractalkine Antibody; Rabbit Polyclonal; Fractalkine/CX3CL1 Antibody; Rabbit PAb; Antigen Affinity Purified; chemokine (C-X3-C motif) ligand 1; Anti-ABCD-3 Antibody; Anti-C3Xkine Antibody; Anti-CXC3 Antibody; Anti-CXC3C Antibody; Anti-fractalkine Antibody; Anti-neurotactin Antibody; Anti-NTN Antibody; Anti-NTT Antibody; Anti-SCYD1 Antibody; anti-CX3CL1 antibody

• Host: Rabbit
• Reactivity: Human
• Clonality: Polyclonal
• Isotype: Rabbit IgG
• Specificity: Human CX3CL1/FKN
• Purity/Purification: Protein A & Antigen Affinity
• Form/Format: Liquid; 0.2um filtered solution in PBS

• Applicable Applications for anti-CX3CL1 antibody: ELISA (EIA), Immunohistochemistry-Paraffin (IHC-P)
• Application Notes: ELISA: 1:1000-1:2000; IHC-P: 1:1000-1:4000
• Immunogen: Recombinant Human CX3CL1 protein
• Conjugation: Unconjugated
• Preparation: Produced in rabbits immunized with purified, recombinant Human CX3CL1 extracellular domain (rhCX3CL1). CX3CL1 specific IgG was purified by human CX3CL1 affinity chromatography.
• Preparation and Storage: This antibody can be stored at 2-8 degree C for one month without detectable loss of activity. Antibody products are stable for twelve months from date of receipt when stored at -20 degree C to -80 degree C. Preservative-Free. Avoid repeated freeze-thaw cycles.

Immunohistochemistry (IHC)

(Immunochemical staining of human CX3CL1 in human kidney with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections).)

Immunohistochemistry (IHC)

(Immunochemical staining of human CX3CL1 in human brain with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections). Positive staining was localized to neuron.)

Immunohistochemistry (IHC)

(Immunochemical staining of human CX3CL1 in human appendix with rabbit polyclonal antibody (1:2000, formalin-fixed paraffin embedded sections). Positive staining was localized to neuron in myenteric nervous plexus. The left panel: tissue incubated with primary antibody; The right panel: tissue incubated with the mixture of primary antibody and antigen (recombinant protein).)

Related Product Information for anti-CX3CL1 antibody

Fractalkine or Chemokine (C-X3-C motif) ligand 1 (CX3CL1) is a member of the CX3C chemokine family. Fractalkine/CX3CL1 is a unique chemokine that functions not only as a chemoattractant but also as an adhesion molecule and is expressed on endothelial cells activated by proinflammatory cytokines, such as interferon-gamma and tumor necrosis factor-alpha. Fractalkine/CX3CL1 is expressed in a membrane-bound form on activated endothelial cells and mediates attachment and firm adhesion of T cells, monocytes and NK cells. Fractalkine/CX3CL1 is associated with dendritic cells (DC) in epidermis and lymphoid organs. The fractalkine receptor, CX3CR1, is expressed on cytotoxic effector lymphocytes, including natural killer (NK) cells and cytotoxic T lymphocytes, which contain high levels of intracellular perforin and granzyme B, and on macrophages. Soluble fractalkine causes migration of NK cells, cytotoxic T lymphocytes, and macrophages, whereas the membrane-bound form captures and enhances the subsequent migration of these cells in response to secondary stimulation with other chemokines.

Product Categories/Family for anti-CX3CL1 antibody

Neuroinflammation

References

Imai T, et al. (1997) Identification and molecular characterization of fractalkine receptor CX3CR1, which mediates both leukocyte migration and adhesion. Cell. 91(4): 521-30.Papadopoulos EJ, et al. (1999) Fractalkine, a CX3C chemokine, is expressed by dendritic cells and is up-regulated upon dendritic cell maturation. Eur J Immunol. 29 (8): 2551-9.Umehara H, et al. (2004) Fractalkine in vascular biology: from basic research to clinical disease". Arterioscler. Thromb Vasc Biol. 24 (1): 34-40.

NCBI and Uniprot Product Information

• NCBI GI #: 4506857
• NCBI GeneID: 6376
• NCBI Accession #: NP_002987.1
• NCBI GenBank Nucleotide #: NP_002987.1
• UniProt Accession #: P78423
• NCBI Official Full Name: fractalkine isoform 1
• NCBI Official Synonym Full Names: C-X3-C motif chemokine ligand 1
• NCBI Official Symbol: CX3CL1
• NCBI Official Synonym Symbols: NTN; NTT; CXC3; CXC3C; SCYD1; ABCD-3; C3Xkine; fractalkine; neurotactin
• NCBI Protein Information: fractalkine
• UniProt Protein Name: Fractalkine
• Protein Family: Fractalkine
• UniProt Gene Name: CX3CL1
• UniProt Synonym Gene Names: FKN; NTT; SCYD1
• UniProt Entry Name: X3CL1_HUMAN

NCBI Description

This gene belongs to the CX3C subgroup of chemokines, characterized by the number of amino acids located between the conserved cysteine residues. This is the only member of the CX3C subgroup, which contains three amino acids between cysteine residues, resulting in a Cys-X-X-X-Cys configuration. The encoded protein contains an extended mucin-like stalk with a chemokine domain on top, and exists in both a membrane-anchored form where it acts as a binding molecule, or, in soluble form, as a chemotactic cytokine. The mature form of this protein can be cleaved at the cell surface, yielding different soluble forms that can interact with the G-protein coupled receptor, C-X3-C motif chemokine receptor 1 gene product. This gene plays a role in a wide range of diseases, including cancer, vasculitis, neuropathies, atherosclerosis, inflammatory diseases, and in human immunodeficiency virus infections. [provided by RefSeq, Sep 2017]

Uniprot Description

CX3CL1: The soluble form is chemotactic for T-cells and monocytes, but not for neutrophils. The membrane-bound form promotes adhesion of those leukocytes to endothelial cells. May play a role in regulating leukocyte adhesion and migration processes at the endothelium. Binds to CX3CR1. Belongs to the intercrine delta family.

Protein type: Motility/polarity/chemotaxis; Membrane protein, integral

Chromosomal Location of Human Ortholog: 16q13

Cellular Component: extracellular space; cell surface; integral to membrane; extracellular region; plasma membrane

Molecular Function: protein binding; chemokine activity; receptor binding

Biological Process: neutrophil chemotaxis; positive regulation of transforming growth factor-beta1 production; leukocyte chemotaxis; cytokine and chemokine mediated signaling pathway; defense response; chemotaxis; angiogenesis involved in wound healing; leukocyte adhesive activation; macrophage chemotaxis; positive regulation of angiogenesis; positive regulation of calcium-independent cell-cell adhesion; immune response; cell adhesion; lymphocyte chemotaxis; positive regulation of inflammatory response