Rabbit Connexin 31 ELISA Kit | CX31 elisa kit

Rabbit Connexin 31 ELISA Kit

Cat. Num. AAA720473

• Gene Names: GJB3; EKV; CX31; DFNA2; DFNA2B
• Reactivity: Rabbit
• Synonyms: Connexin 31; Rabbit Connexin 31 ELISA Kit; CX31 elisa kit

• Reactivity: Rabbit

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Detection Range: 250-5000pg/mL
• Sensitivity: 1.0pg/mL
• Intended Uses: This CX31 ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Rabbit CX31. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CX31 elisa kit

Principle of the assay: CX31 ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CX31 antibody and an CX31-HRP conjugate. The assay sample and buffer are incubated together with CX31-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CX31 concentration since CX31 from samples and CX31-HRP conjugate compete for the anti-CX31 antibody binding site. Since the number of sites is limited, as more sites are occupied by CX31 from the sample, fewer sites are left to bind CX31-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CX31 concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CX31 elisa kit

Cell Biology

NCBI and Uniprot Product Information

• NCBI GI #: 3982600
• NCBI GeneID: 2707
• Molecular Weight: 30,818 Da
• NCBI Official Full Name: connexin 31
• NCBI Official Synonym Full Names: gap junction protein, beta 3, 31kDa
• NCBI Official Symbol: GJB3
• NCBI Official Synonym Symbols: EKV; CX31; DFNA2; DFNA2B
• NCBI Protein Information: gap junction beta-3 protein; connexin 31; connexin-31
• UniProt Protein Name: Gap junction beta-3 protein
• UniProt Gene Name: GJB3
• UniProt Synonym Gene Names: CX31; Cx31
• UniProt Entry Name: CXB3_HUMAN

NCBI Description

This gene is a member of the connexin gene family. The encoded protein is a component of gap junctions, which are composed of arrays of intercellular channels that provide a route for the diffusion of low molecular weight materials from cell to cell. Mutations in this gene can cause non-syndromic deafness or erythrokeratodermia variabilis, a skin disorder. Alternative splicing results in multiple transcript variants encoding the same protein. [provided by RefSeq, Jul 2008]

Uniprot Description

GJB3: One gap junction consists of a cluster of closely packed pairs of transmembrane channels, the connexons, through which materials of low MW diffuse from one cell to a neighboring cell. Defects in GJB3 are a cause of erythrokeratodermia variabilis (EKV). EKV is a genodermatosis characterized by the appearance of two independent skin lesions: transient figurate erythematous patches and hyperkeratosis that is usually localized but occasionally occurs in its generalized form. Clinical presentation varies significantly within a family and from one family to another. Palmoplantar keratoderma is present in around 50% of cases. Defects in GJB3 are the cause of deafness autosomal dominant type 2B (DFNA2B). DFNA2 is a form of sensorineural hearing loss. Sensorineural deafness results from damage to the neural receptors of the inner ear, the nerve pathways to the brain, or the area of the brain that receives sound information. Belongs to the connexin family. Beta-type (group I) subfamily.

Protein type: Channel, misc.; Membrane protein, integral; Membrane protein, multi-pass

Chromosomal Location of Human Ortholog: 1p34

Cellular Component: connexon complex; cytoplasm; integral to membrane; gap junction

Molecular Function: gap junction channel activity

Biological Process: skin development; sensory perception of sound; in utero embryonic development; cell communication; transmembrane transport; placenta development

Disease: Deafness, Autosomal Recessive 1a; Deafness, Autosomal Dominant 2b; Erythrokeratodermia Variabilis Et Progressiva