Goat Cytovillin ELISA Kit | CVL elisa kit

Goat Cytovillin ELISA Kit

Cat. Num. AAA746862

• Gene Names: EZR; CVL; CVIL; VIL2; HEL-S-105
• Reactivity: Goat
• Synonyms: Cytovillin; Goat Cytovillin ELISA Kit; CVL elisa kit

• Reactivity: Goat

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Intended Uses: This CVL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CVL. This ELISA kit for research use only, not for therapeutic applications
• !Intended Uses: This CVL ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Goat CVL. This ELISA kit for research use only, not for therapeutic applications!
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CVL elisa kit

Principle of the Assay: CVL ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CVL antibody and an CVL-HRP conjugate. The assay sample and buffer are incubated together with CVL-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CVL concentration since CVL from samples and CVL-HRP conjugate compete for the anti-CVL antibody binding site. Since the number of sites is limited, as more sites are occupied by CVL from the sample, fewer sites are left to bind CVL-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CVL concentration in each sample is interpolated from this standard curve.

Product Categories/Family for CVL elisa kit

Signal Transduction

NCBI and Uniprot Product Information

• NCBI GI #: 161702986
• NCBI GeneID: 7430
• NCBI Accession #: NP_001104547.1
• NCBI GenBank Nucleotide #: NM_001111077.1
• Molecular Weight: 69,413 Da
• NCBI Official Full Name: ezrin
• NCBI Official Synonym Full Names: ezrin
• NCBI Official Symbol: EZR
• NCBI Official Synonym Symbols: CVL; CVIL; VIL2; HEL-S-105
• NCBI Protein Information: ezrin; p81; villin-2; cytovillin 2; villin 2 (ezrin); epididymis secretory protein Li 105
• UniProt Protein Name: Ezrin
• UniProt Gene Name: EZR
• UniProt Synonym Gene Names: VIL2
• UniProt Entry Name: EZRI_HUMAN

NCBI Description

The cytoplasmic peripheral membrane protein encoded by this gene functions as a protein-tyrosine kinase substrate in microvilli. As a member of the ERM protein family, this protein serves as an intermediate between the plasma membrane and the actin cytoskeleton. This protein plays a key role in cell surface structure adhesion, migration and organization, and it has been implicated in various human cancers. A pseudogene located on chromosome 3 has been identified for this gene. Alternatively spliced variants have also been described for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

Ezrin: a member of the ERM family which includes radixin and moesin. ERM proteins appear to function as cross-linkers between plasma membranes and actin-based cytoskeletons. Plays a key role in cell surface structure adhesion, migration, and organization.

Protein type: Motility/polarity/chemotaxis; Cytoskeletal

Chromosomal Location of Human Ortholog: 6q25.3

Cellular Component: cortical cytoskeleton; extracellular space; microvillus; focal adhesion; basolateral plasma membrane; T-tubule; actin filament; cytosol; actin cytoskeleton; lipid raft; ruffle; extrinsic to membrane; membrane; apical part of cell; microvillus membrane; apical plasma membrane; nucleolus; plasma membrane; uropod; vesicle; filopodium

Molecular Function: actin filament binding; protein domain specific binding; protein binding; cell adhesion molecule binding

Biological Process: microvillus biogenesis; actin filament bundle formation; axon guidance; regulation of cell shape; leukocyte adhesion; filopodium formation; cytoskeletal anchoring; receptor internalization; membrane to membrane docking; establishment of epithelial cell polarity