RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine Culture Media
RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine (Powder)
Purity
Cell Culture Grade
Synonyms
RPMI 1640 Medium Deficient w/L-Glutamine; w/o Isoleucine; w/o Isoleucine (Powder); w/o Isoleucine culture media
Purity/Purification
Cell Culture Grade
Form/Format
Light orange, homogeneous, free flowing powder
pH
8.3
Solubility
Pink, clear, complete
Endotoxin Level
≤ 1EU/ml
Directions per liter
Dissolve 10.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Adjust pH of the medium to the desired level. Add 2g/L sodium bicarbonate with stirring. Add additional water to bring the solution to1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Note
It may be necessary to lower the pH to 4.0 with 1N HCl to completely dissolve this product. After it has dissolved completely, the pH can be raised to 7.2 with 1N NaOH prior to the addition of sodium bicarbonate.
Components shown as g/liter
Inorganic Salts:
Calcium Nitrate•4H2O: 0.10
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.40
Sodium Chloride: 6.00
Sodium Phosphate Dibasic: 0.80
Amino Acids:
L-Arginine: 0.20
L-Asparagine: 0.05
L-Aspartic Acid: 0.02
L-Cystine•2HCl: 0.0652
L-Glutamic Acid: 0.02
L-Glutamine: 0.30
Glycine: 0.01
L-Histidine: 0.015
Hydroxy-L-proline: 0.02
L-Isoleucine- absent
L-Leucine: 0.05
L-Lysine•HCl: 0.04
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.02
L-Serine: 0.03
L-Threonine: 0.02
L-Tryptophan: 0.005
L-Tyrosine•2Na•2H2O: 0.02883
L-Valine: 0.02
Vitamins:
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid•Ca: 0.00025
Pyridoxine•HCl: 0.001
Riboflavin: 0.0002
Thiamine•HCl: 0.001
Other:
D-Glucose: 2.0
Glutathione: 0.001
Phenol Red, sodium: 0.0053
Calcium Nitrate•4H2O: 0.10
Magnesium Sulfate: 0.04884
Potassium Chloride: 0.40
Sodium Chloride: 6.00
Sodium Phosphate Dibasic: 0.80
Amino Acids:
L-Arginine: 0.20
L-Asparagine: 0.05
L-Aspartic Acid: 0.02
L-Cystine•2HCl: 0.0652
L-Glutamic Acid: 0.02
L-Glutamine: 0.30
Glycine: 0.01
L-Histidine: 0.015
Hydroxy-L-proline: 0.02
L-Isoleucine- absent
L-Leucine: 0.05
L-Lysine•HCl: 0.04
L-Methionine: 0.015
L-Phenylalanine: 0.015
L-Proline: 0.02
L-Serine: 0.03
L-Threonine: 0.02
L-Tryptophan: 0.005
L-Tyrosine•2Na•2H2O: 0.02883
L-Valine: 0.02
Vitamins:
p-Aminobenzoic Acid: 0.001
D-Biotin: 0.0002
Choline Chloride: 0.003
Cyanocobalamin: 0.000005
Folic Acid: 0.001
myo-Inositol: 0.035
Niacinamide: 0.001
D-Pantothenic Acid•Ca: 0.00025
Pyridoxine•HCl: 0.001
Riboflavin: 0.0002
Thiamine•HCl: 0.001
Other:
D-Glucose: 2.0
Glutathione: 0.001
Phenol Red, sodium: 0.0053
Total
10.3g/Liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4 degree C and used within a short period of time.
Related Product Information for RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine culture media
RPMI 1640 is used for the culture of human normal and neoplastic leukocytes. RPMI 1640 was developed by Moore et. al. at Roswell Park Memorial Institute.
Product Categories/Family for RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine culture media
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Product Notes
The RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine (Catalog #AAA653278) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "RPMI 1640 Medium Deficient w/L-Glutamine, w/o Isoleucine, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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