MCDB 110 Diploid Fibroblast-like Cell Medium Culture Media
MCDB 110 Diploid Fibroblast-like Cell Medium (Powder)
Purity
Cell Culture Grade
Synonyms
MCDB 110 Diploid Fibroblast-like Cell Medium; MCDB 110 Diploid Fibroblast-like Cell Medium (Powder); MCDB 110 Diploid Fibroblast-like Cell Medium culture media
Purity/Purification
Cell Culture Grade
Form/Format
White to light orange, homogeneous, free flowing powder
Appearance
White, homogeneous, free flowing powder
Endotoxin Level
1EU/ml
Solubility (15.3 g/L)
Pale yellow, clear, complete
pH
6.5
Directions per Liter
Dissolve 15.3g in 800-900ml of ddH2O stirring gently until completely solubilized. Add sodium bicarbonate if required.Adjust pH of the medium to the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/liters
Inorganic Salts:
Ammonium Metavandate: 0.000585
Ammonium Molybdate: 0.00124
Calcium Chloride•2H20: 147.00
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.39
Magnesium Sulfate: 120.38
Manganese Sulfate: 0.000151
Nickel Chloride Hexahydrate: 0.00012
Potassium Chloride: 372.75
Sodium Chloride: 6546.00
Sodium Metasilicate•9H2O: 0.1421
Sodium Phosphate Dibasic: 425.94
Sodium Selenite: 0.0038
Stannous Chloride Dihydrate: 0.000113
Zinc Sulfate•7H2O : 0.144
Amino Acids:
L-Alanine: 8.90
L-Arginine HCl: 210.70
L-Asparagine H2O: 15.00
L-Aspartic Acid: 13.31
L-Cysteine HCl: H2O 8.78
L-Glutamic Acid: 14.71
L-Glutamine: 365.30
Glycine: 22.52
L-Histidine HCl: H2O 20.97
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.04
L-Tyrosine: 7.84
L-Valine: 11.72
Vitamins:
Biotin: 0.00733
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.000602
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.2383
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337
Other:
Adenine HCl: 1.72
Thymidine: 0.0727
D-Glucose: 720.64
HEPES Free Acid: 5958.00
Thioctic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine•2HCl: 0.0001611
Sodium Pyruvate: 110.00
Total: 15.3g/liter
Ammonium Metavandate: 0.000585
Ammonium Molybdate: 0.00124
Calcium Chloride•2H20: 147.00
Cupric Sulfate•5H20: 0.00025
Ferrous Sulfate•7H20: 1.39
Magnesium Sulfate: 120.38
Manganese Sulfate: 0.000151
Nickel Chloride Hexahydrate: 0.00012
Potassium Chloride: 372.75
Sodium Chloride: 6546.00
Sodium Metasilicate•9H2O: 0.1421
Sodium Phosphate Dibasic: 425.94
Sodium Selenite: 0.0038
Stannous Chloride Dihydrate: 0.000113
Zinc Sulfate•7H2O : 0.144
Amino Acids:
L-Alanine: 8.90
L-Arginine HCl: 210.70
L-Asparagine H2O: 15.00
L-Aspartic Acid: 13.31
L-Cysteine HCl: H2O 8.78
L-Glutamic Acid: 14.71
L-Glutamine: 365.30
Glycine: 22.52
L-Histidine HCl: H2O 20.97
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine HCl: 36.54
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.04
L-Tyrosine: 7.84
L-Valine: 11.72
Vitamins:
Biotin: 0.00733
Choline Chloride: 13.96
Vitamin B12: 0.136
Folinic Acid, Calcium: 0.000602
Myo-Inositol: 18.02
Nicotinamide: 6.11
D-Pantothenic Acid•Ca: 0.2383
Pyridoxine•HCl: 0.0617
Riboflavin: 0.113
Thiamine•HCl: 0.337
Other:
Adenine HCl: 1.72
Thymidine: 0.0727
D-Glucose: 720.64
HEPES Free Acid: 5958.00
Thioctic Acid: 0.00206
Phenol Red, Sodium: 1.242
Putrescine•2HCl: 0.0001611
Sodium Pyruvate: 110.00
Total: 15.3g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should becapped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for MCDB 110 Diploid Fibroblast-like Cell Medium culture media
Basal Medium MCDB 110 is specifically formulated for the cultivation of diploid fibroblast-like cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).
Product Categories/Family for MCDB 110 Diploid Fibroblast-like Cell Medium culture media
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Product Notes
The MCDB 110 Diploid Fibroblast-like Cell Medium (Catalog #AAA652776) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "MCDB 110 Diploid Fibroblast-like Cell Medium, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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