Endothelial Basal Medium MCDB 107 Culture Media
Endothelial Basal Medium MCDB 107 (Powder)
Purity
Cell Culture Grade
Synonyms
Endothelial Basal Medium MCDB 107; Endothelial Basal Medium MCDB 107 (Powder); Endothelial Basal Medium MCDB 107 culture media
Purity/Purification
Cell Culture Grade
Appearance
Light orange, homogenous, free flowing powder
Solubility
Yellow, clear, completer
pH
5.8
Endotoxin
< = 1EU/ml
Note
Based powder without supplements.
Directions per Liter
Dissolve 16.2g in 800-900ml of ddH2O, stirring gently until completely solubilized. Add 1.2g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as mg/liter
Inorganic Salts
Calcium Chloride•2H2O: 111
Cupric Sulfate•5H2O: 0.0002497
Ferric Nitrate•9H2O: 1.39
Potassium Chloride: 149
Potassium phosphate mono: 408
Magnesium Sulfate: 120.38
Manganese Sulfate•5H2O: 0.00241
Sodium Chloride: 6599.2
Selenite•5H2O: 0.00789
Sodium Metasilicate•9H2O: 0.1421
Ammonium Molybdate•4H2O: 0.0012359
Ammonium Metavandate: 0.000585
Nickel Chloride•6H2O: 0.00011885
Stannous Chloride•2H2O: 0.0001128
Zinc Sulfate•7H2O: 0.14375
Amino Acids
L-Alanine: 8.9
L-Arginine: 174.2
L-Asparagine: 13.21
L-Aspartic Acid: 13.31
L-Cysteine: 6.11
L-Glutamic Acid: 14.71
L-Glutamine: 365
Glycine: 22.53
L-Histidine: 15.52
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine: 29.24
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.042
L-Tyrosine: 5.436
L-Valine: 11.72
Vitamins
Biotin: 0.007329
D-Pantothenate, Ca: 0.2383
Choline Chloride: 13.96
Folinic Acid, Ca: 0.0006016
Myo-Inositol: 18.02
Nicotinamide: 6.105
Pyridoxine•HCl: 0.02056
Riboflavin: 0.11292
Thiamine•HCl: 0.3373
Vitamin B12: 0.13554
Other
Adenine: 1.35
D-glucose: 720.8
HEPES Buffer: 7149
Lipoic Acid: 0.002063
Phenol Red, Na: 1.129
Putrescine•2HCI: 0.0001611
Sodium Pyruvate: 110
Thymidine: 0.07266
Total: 16.2g/liter
Calcium Chloride•2H2O: 111
Cupric Sulfate•5H2O: 0.0002497
Ferric Nitrate•9H2O: 1.39
Potassium Chloride: 149
Potassium phosphate mono: 408
Magnesium Sulfate: 120.38
Manganese Sulfate•5H2O: 0.00241
Sodium Chloride: 6599.2
Selenite•5H2O: 0.00789
Sodium Metasilicate•9H2O: 0.1421
Ammonium Molybdate•4H2O: 0.0012359
Ammonium Metavandate: 0.000585
Nickel Chloride•6H2O: 0.00011885
Stannous Chloride•2H2O: 0.0001128
Zinc Sulfate•7H2O: 0.14375
Amino Acids
L-Alanine: 8.9
L-Arginine: 174.2
L-Asparagine: 13.21
L-Aspartic Acid: 13.31
L-Cysteine: 6.11
L-Glutamic Acid: 14.71
L-Glutamine: 365
Glycine: 22.53
L-Histidine: 15.52
L-Isoleucine: 3.94
L-Leucine: 13.12
L-Lysine: 29.24
L-Methionine: 4.48
L-Phenylalanine: 4.96
L-Proline: 34.53
L-Serine: 10.51
L-Threonine: 11.91
L-Tryptophan: 2.042
L-Tyrosine: 5.436
L-Valine: 11.72
Vitamins
Biotin: 0.007329
D-Pantothenate, Ca: 0.2383
Choline Chloride: 13.96
Folinic Acid, Ca: 0.0006016
Myo-Inositol: 18.02
Nicotinamide: 6.105
Pyridoxine•HCl: 0.02056
Riboflavin: 0.11292
Thiamine•HCl: 0.3373
Vitamin B12: 0.13554
Other
Adenine: 1.35
D-glucose: 720.8
HEPES Buffer: 7149
Lipoic Acid: 0.002063
Phenol Red, Na: 1.129
Putrescine•2HCI: 0.0001611
Sodium Pyruvate: 110
Thymidine: 0.07266
Total: 16.2g/liter
Preparation and Storage
Store powdered media at RT. Opened bottles should be capped tightly and kept in a dark, low humidity environment. Prepared media should be kept at 4°C and used within a short period of time.
Related Product Information for Endothelial Basal Medium MCDB 107 culture media
Basal Medium for the growth of nonendothelial mesenchymal cells. Supplement with growth factors or fetal calf serum (0.2-2.0% dialyzed).
Product Categories/Family for Endothelial Basal Medium MCDB 107 culture media
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Product Notes
The Endothelial Basal Medium MCDB 107 (Catalog #AAA652891) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Endothelial Basal Medium MCDB 107, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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