Dulbecco's MEM Culture Media
Dulbecco's MEM (DMEM) w/o Glucose, Methionine, Cystine, Histidine (Powder)
Purity
Cell Culture Grade
Synonyms
Dulbecco's MEM; Dulbecco's MEM (DMEM) w/o Glucose; Methionine; Cystine; Histidine (Powder); Dulbecco's MEM culture media
Purity/Purification
Cell Culture Grade
Form/Format
Orange, homogenous, free flowing powder
Endotoxin Level
1EU/ml
pH
6.4
Solubility
Orange, clear, complete
Directions per Liter
Dissolve 8.85g in 800-900ml of ddH2O, stirring gently until completely solubilized. If required, add 3.7g sodium bicarbonate with stirring. Adjust pH of the medium to 0.1-0.3 pH unit below the desired level. Add additional water to bring the solution to 1L. Filter-sterilize using a 0.22 micron membrane filter. Aliquot into sterile containers. Do not autoclave. Contains heat-labile compounds that can be damaged with autoclaving.
Components shown as g/Liter
Inorganic Salts:
Calcium Chloride, anhy 0.200
Ferric Nitrate•9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic•H2O 0.125
Amino Acids:
L-Arginine•HCl 0.084
L-Cystine Absent
L-Glutamine 0.584
Glycine 0.03
L-Histidine•HCl•H2O Absent
L-Isoleucine 0.105
L-Leucine 0.105
L-Lysine•HCl 0.146
L-Methionine Absent
L-Phenylalanine 0.066
L-Serine 0.042
L-Threonine 0.095
L-Tryptophan 0.016
L-Tyrosine•2Na•2H2O 0.104
L-Valine 0.094
Vitamins:
Choline Chloride 0.004
Folic Acid 0.004
i-Inositol 0.0072
Niacinamide 0.004
D-Pantothenic Acid•Ca 0.004
Pyridoxal•HCl 0.004
Riboflavin 0.0004
Thiamine•HCl 0.004
Other:
D-Glucose Absent
Phenol Red, Sodium 0.015
Pyruvic Acid, Sodium 0.110
Total: 8.85g/Liter
Calcium Chloride, anhy 0.200
Ferric Nitrate•9H2O 0.0001
Magnesium Sulfate 0.09767
Potassium Chloride 0.4
Sodium Chloride 6.4
Sodium Phosphate Monobasic•H2O 0.125
Amino Acids:
L-Arginine•HCl 0.084
L-Cystine Absent
L-Glutamine 0.584
Glycine 0.03
L-Histidine•HCl•H2O Absent
L-Isoleucine 0.105
L-Leucine 0.105
L-Lysine•HCl 0.146
L-Methionine Absent
L-Phenylalanine 0.066
L-Serine 0.042
L-Threonine 0.095
L-Tryptophan 0.016
L-Tyrosine•2Na•2H2O 0.104
L-Valine 0.094
Vitamins:
Choline Chloride 0.004
Folic Acid 0.004
i-Inositol 0.0072
Niacinamide 0.004
D-Pantothenic Acid•Ca 0.004
Pyridoxal•HCl 0.004
Riboflavin 0.0004
Thiamine•HCl 0.004
Other:
D-Glucose Absent
Phenol Red, Sodium 0.015
Pyruvic Acid, Sodium 0.110
Total: 8.85g/Liter
Preparation and Storage
RT/4°C
Related Product Information for Dulbecco's MEM culture media
Dulbecco's MEM is the most widely used modification of BME. It contains a 4-fold higher concentration of amino acids and vitamins. Non-essential amino acids and certain essential trace elements were added. The bicarbonate concentration was increased. L-Glutamine and Glucose are absent. The standard formula for DMEM is with 1000mg/ml glucose. DMEM was originally developed for the culture of mice embryonic cells. Today, it finds a broad application of serum free culture of normal and transformed mouse and chicken cells.
Product Categories/Family for Dulbecco's MEM culture media
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Product Notes
The Dulbecco's MEM (Catalog #AAA652805) is a Culture Media and is intended for research purposes only. The product is available for immediate purchase. It is sometimes possible for the material contained within the vial of "Dulbecco's MEM, Culture Media" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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