Rabbit anti-Human, Mouse IKK-epsilon Polyclonal Antibody | anti-IKK-epsilon antibody

IKK-epsilon (Phospho-Ser172) Antibody

Cat. Num. AAA9416596

• Gene Names: IKBKE; IKKE; IKKI; IKK-E; IKK-i
• Reactivity: Human, Mouse
• Applications: Western Blot
• Purity: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Synonyms: IKK-epsilon; Polyclonal Antibody; IKK-epsilon (Phospho-Ser172) Antibody; IKBKE; Ikb kinase epsilon; IKK-E; Inducible I kappa-B kinase; KIAA0151; IKK-i; IKK-related kinase epsilon; I-kappa-B kinase epsilon; IKKE; IKKepsilon; IKKI; Inducible IkappaB kinase; anti-IKK-epsilon antibody

• Host: Rabbit
• Reactivity: Human, Mouse
• Clonality: Polyclonal
• Specificity: IKK-eta (Phospho-Ser172) Antibody detects endogenous levels of IKK-eta only when phosphorylated at Ser172
• Purity/Purification: Antibodies were produced by immunizing rabbits with synthetic phosphopeptide and KLH conjugates. Antibodies were purified by affinity-chromatography using epitope-specific phosphopeptide. Non-phospho specific antibodies were removed by chromatogramphy usi
• Form/Format: Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.
• Concentration: 1 mg/ml (varies by lot)
• Sequence Length: 631

• Applicable Applications for anti-IKK-epsilon antibody: Western Blot (WB)
• Application Notes: Western Blot: 1:1000
• Immunogen: A synthesized peptide derived from human IKK-eta (Phospho-Ser172)
• Immunogen Type: Peptide
• Modification: Phospho-Ser172
• Preparation and Storage: Store at -20 degree C

Western Blot (WB)

(Western blot analysis IKK-eta (Phospho-Ser172) using Na3VO4 treated HepG2 whole cell lysates)

Product Categories/Family for anti-IKK-epsilon antibody

Phospho-specific Ab

NCBI and Uniprot Product Information

• NCBI GI #: 300863151
• NCBI GeneID: 9641
• NCBI Accession #: NP_001180250.1
• NCBI GenBank Nucleotide #: NM_001193321.1
• UniProt Accession #: Q14164; Q3B754; Q3KR43; Q5JTS6; D3DT78
• Molecular Weight: 70,816 Da
• NCBI Official Full Name: inhibitor of nuclear factor kappa-B kinase subunit epsilon isoform 3
• NCBI Official Synonym Full Names: inhibitor of kappa light polypeptide gene enhancer in B-cells, kinase epsilon
• NCBI Official Symbol: IKBKE
• NCBI Official Synonym Symbols: IKKE; IKKI; IKK-E; IKK-i
• NCBI Protein Information: inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Protein Name: Inhibitor of nuclear factor kappa-B kinase subunit epsilon
• UniProt Gene Name: IKBKE
• UniProt Synonym Gene Names: IKKE; IKKI; KIAA0151; I-kappa-B kinase epsilon; IKK-E; IKK-epsilon; IkBKE; IKK-i
• UniProt Entry Name: IKKE_HUMAN

NCBI Description

IKBKE is a noncanonical I-kappa-B (see MIM 164008) kinase (IKK) that is essential for regulating antiviral signaling pathways. IKBKE has also been identified as a breast cancer (MIM 114480) oncogene and is amplified and overexpressed in over 30% of breast carcinomas and breast cancer cell lines (Hutti et al., 2009 [PubMed 19481526]).[supplied by OMIM, Oct 2009]

Uniprot Description

IKKE: a oncogenic kinase of the IKK family. Phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. Plays a pivotal role in coordinating the activation of IRF3 and NF-kappaB in the innate immune response. Its redistribution to PML nuclear bodies upon DNA damage is TOPORS-dependent. Is a breast cancer oncogene that exerts its oncogenic properties through cRel.

Protein type: EC 2.7.11.10; IKK family; Inhibitor; Kinase, protein; Other group; Protein kinase, Other; Protein kinase, Ser/Thr (non-receptor)

Chromosomal Location of Human Ortholog: 1q32.1

Cellular Component: cytoplasm; cytosol; endosome membrane; mitochondrial membrane; nucleus; PML body

Molecular Function: NF-kappaB-inducing kinase activity; protein binding; protein kinase activity; ubiquitin protein ligase binding

Biological Process: DNA damage response, signal transduction resulting in induction of apoptosis; innate immune response; negative regulation of interferon type I production; peptidyl-serine phosphorylation; positive regulation of I-kappaB kinase/NF-kappaB cascade; protein amino acid phosphorylation; protein homooligomerization