Rat Cubilin ELISA Kit | Cubn elisa kit

Rat Cubilin ELISA Kit

Cat. Num. AAA764305

• Gene Names: Cubn; IFCR; MGA1; GP280
• Reactivity: Rat
• Synonyms: Cubilin; Rat Cubilin ELISA Kit; Cubn/gp280/IFCR/MGA1/cubilin (intrinsic factor-cobalamin receptor)/gp280/IFCR/MGA1; Cubn elisa kit

• Reactivity: Rat
• Specificity: This assay has high sensitivity and excellent specificity for detection of CUBN. No significant cross-reactivity or interference between CUBN and analogues was observed.
• Sequence Length: 3623

• Samples: Serum, plasma, tissue homogenates and other biological fluids
• Assay Type: Quantitative Sandwich
• Detection Range: 0.156-10ng/ml
• Sensitivity: 0.094ng/ml
• Intra-assay Precision: CV<8%
• Inter-assay Precision: CV<10%
• Preparation and Storage: Store at 4 degree C if kit is to be used within 1 week. Stable for 6 months (if micro ELISA Plate, Lyophilized Standard and Concentrated Biotinylated Detection Protein stored at-20 degree C. Other components at 2-8 degree C). Stable for 12 months (if the entire kit is stored at-20 degree C).

Typical Testing Data/Standard Curve (for reference only)

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Related Product Information for Cubn elisa kit

Principle of the Assay: This kit was based on sandwich enzyme-linked immune-sorbent assay technology. Capture antibody was pre-coated onto 96-well plates. And the biotin conjugated antibody was used as detection antibodies. The standards, test samples and biotin conjugated detection antibody were added to the wells subsequently, and washed with wash buffer. HRP-Streptavidin was added and unbound conjugates were washed away with wash buffer. TMB substrates were used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the target amount of sample captured in plate. Read the O.D. absorbance at 450nm in a microplate reader, and then the concentration of target can be calculated.

NCBI and Uniprot Product Information

• NCBI GI #: 16758040
• NCBI GeneID: 80848
• NCBI Accession #: NP_445784.1
• NCBI GenBank Nucleotide #: NM_053332.2
• Molecular Weight: 398,987 Da
• NCBI Official Full Name: cubilin
• NCBI Official Synonym Full Names: cubilin
• NCBI Official Symbol: Cubn
• NCBI Official Synonym Symbols: IFCR; MGA1; GP280
• NCBI Protein Information: cubilin
• UniProt Protein Name: Cubilin
• Protein Family: Cubilin
• UniProt Gene Name: Cubn
• UniProt Synonym Gene Names: Ifcr; gp280
• UniProt Entry Name: CUBN_RAT

NCBI Description

receptor for intrinsic factor (IF)-cobalamin; involved in vitamin B12 uptake (cyanocobalamin) [RGD, Feb 2006]

Uniprot Description

CUBN: Cotransporter which plays a role in lipoprotein, vitamin and iron metabolism, by facilitating their uptake. Binds to ALB, MB, Kappa and lambda-light chains, TF, hemoglobin, GC, SCGB1A1, APOA1, high density lipoprotein, and the GIF-cobalamin complex. The binding of all ligands requires calcium. Serves as important transporter in several absorptive epithelia, including intestine, renal proximal tubules and embryonic yolk sac. Interaction with LRP2 mediates its trafficking throughout vesicles and facilitates the uptake of specific ligands like GC, hemoglobin, ALB, TF and SCGB1A1. Interaction with AMN controls its trafficking to the plasma membrane and facilitates endocytosis of ligands. May play an important role in the development of the peri-implantation embryo through internalization of APOA1 and cholesterol. Binds to LGALS3 at the maternal-fetal interface. Defects in CUBN are a cause of recessive hereditary megaloblastic anemia 1 (RH-MGA1); also known as MGA1 Norwegian type or Imerslund-Grasbeck syndrome (I-GS). RH-MGA1 is due to selective malabsorption of vitamin B12. Defects in vitamin B12 absorption lead to impaired function of thymidine synthase. As a consequence DNA synthesis is interrupted. Rapidly dividing cells involved in erythropoiesis are particularly affected.

Cellular Component: apical part of cell; apical plasma membrane; brush border; brush border membrane; coated pit; cytoplasm; endocytic vesicle; endoplasmic reticulum; endosome; endosome membrane; Golgi apparatus; lysosomal lumen; lysosomal membrane; membrane; protein complex

Molecular Function: calcium ion binding; cobalamin binding; cobalamin transporter activity; hemoglobin binding; identical protein binding; protein homodimerization activity; receptor activity

Biological Process: cholesterol metabolic process; cobalamin transport; hemoglobin import; in utero embryonic development; lipoprotein transport; receptor-mediated endocytosis; response to nutrient; vitamin metabolic process