IFI30 recombinant protein

IFI30, 58-232aa, Human, His tag, E Coli

Cat. Num. AAA204459

• Gene Names: IFI30; GILT; IP30; IP-30; IFI-30
• Applications: SDS-Page
• Purity: > 90% by SDS-PAGE
• Synonyms: IFI30; 58-232aa; Human; His tag; E Coli; Gamma-interferon-inducible lysosomal thiol reductase; GILT; IFI-30; IP30; IFI30 recombinant protein

• Host: E Coli
• Purity/Purification: > 90% by SDS-PAGE
• Form/Format: Liquid. In 20mM Tris-HCl Buffer (pH 8.0) containing 0.1M NaCl, 10% glycerol, 1mM DTT
• Concentration: 1 mg/ml (determined by Bradford assay) (varies by lot)
• Sequence: MGSSHHHHHH SSGLVPRGSH MGSMNAPLVN VTLYYEALCG GCRAFLIREL FPTWLLVMEI LNVTLVPYGN AQEQNVSGRW EFKCQHGEEE CKFNKVEACV LDELDMELAF LTIVCMEEFE DMERSLPLCL QLYAPGLSPD TIMECAMGDR GMQLMHANAQ RTDALQPPHE YVPWVTVNGK PLEDQTQLLT LVCQLYQGK
• Sequence Length: 250

• Applicable Applications for IFI30 recombinant protein: SDS-PAGE
• Antigen Species: Human
• Tag Information: His-tag
• Preparation and Storage: Can be stored at 4 degree C short term (1-2 weeks).; For long term storage, aliquot and store at -20 degree C or -70 degree C.; Avoid repeated freezing and thawing cycles.

SDS-Page

Related Product Information for IFI30 recombinant protein

IFI30, also as known as gamma-interferon-inducible lysosomal thiol reductase, belongs to the GILT family. This protein is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. This protein facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds. Also facilitates MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or cross-presentation. Recombinant human IFI30 protein, fused to His-tag at N-terminus, was expressed in E Coli and purified by conventional column chromatography, after refolding of the isolated inclusion bodies in a renaturation buffer.

Product Categories/Family for IFI30 recombinant protein

Immunology

References

Arunachalam B, et al. (2000) Proc Natl Acad Sci u S A 97 (2): 745-50. ; Luster AD, et al. (1988) J Biol Chem 263 (24): 12036-43.

NCBI and Uniprot Product Information

• NCBI GI #: 29150261
• NCBI GeneID: 10437
• NCBI Accession #: NP_006323
• NCBI GenBank Nucleotide #: NM_006332.4
• UniProt Accession #: P13284; Q76MF9; Q8NEI4; Q8WU77; Q9UL08
• Molecular Weight: 22.5 kDa (199aa) confirmed by MALDI-TOF
• NCBI Official Full Name: gamma-interferon-inducible lysosomal thiol reductase preproprotein
• NCBI Official Synonym Full Names: IFI30, lysosomal thiol reductase
• NCBI Official Symbol: IFI30
• NCBI Official Synonym Symbols: GILT; IP30; IP-30; IFI-30
• NCBI Protein Information: gamma-interferon-inducible lysosomal thiol reductase
• UniProt Protein Name: Gamma-interferon-inducible lysosomal thiol reductase
• Protein Family: Gamma-interferon-inducible lysosomal thiol reductase
• UniProt Gene Name: IFI30
• UniProt Synonym Gene Names: GILT; IP30

NCBI Description

The protein encoded by this gene is a lysosomal thiol reductase that at low pH can reduce protein disulfide bonds. The enzyme is expressed constitutively in antigen-presenting cells and induced by gamma-interferon in other cell types. This enzyme has an important role in MHC class II-restricted antigen processing. [provided by RefSeq, Jul 2008]

Uniprot Description

Lysosomal thiol reductase that can reduce protein disulfide bonds. May facilitate the complete unfolding of proteins destined for lysosomal degradation. Plays an important role in antigen processing. Facilitates the generation of MHC class II-restricted epitodes from disulfide bond-containing antigen by the endocytic reduction of disulfide bonds (). Facilitates also MHC class I-restricted recognition of exogenous antigens containing disulfide bonds by CD8+ T-cells or crosspresentation ().