Cathepsin F Recombinant Protein | CTSF recombinant protein

Recombinant human Cathepsin F protein

Cat. Num. AAA201339

• Gene Names: CTSF; CATSF; CLN13
• Applications: SDS-Page
• Purity: >90% by SDS-PAGE
• Synonyms: Cathepsin F; Recombinant human Cathepsin F protein; CTSF; CATSF; CLN13; CTSF recombinant protein

• Host: Baculovirus
• Purity/Purification: >90% by SDS-PAGE
• Form/Format: Liquid. In Phosphate-Buffered Saline (pH7.4) containing 40% glycerol
• Concentration: 0.25mg/ml (determined by Absorbance at 280nm) (varies by lot)
• Sequence Positions: 20-484aa
• Sequence: APAQPRA ASFQAWGPPS PELLAPTRFA LEMFNRGRAA GTRAVLGLVR GRVRRAGQGS LYSLEATLEE PPCNDPMVCR LPVSKKTLLC SFQVLDELGR HVLLRKDCGP VDTKVPGAGE PKSAFTQGSA MISSLSQNHP DNRNETFSSV ISLLNEDPLS QDLPVKMASI FKNFVITYNR TYESKEEARW RLSVFVNNMV RAQKIQALDR GTAQYGVTKF SDLTEEEFRT IYLNTLLRKE PGNKMKQAKS VGDLAPPEWD WRSKGAVTKV KDQGMCGSCW AFSVTGNVEG QWFLNQGTLL SLSEQELLDC DKMDKACMGG LPSNAYSAIK NLGGLETEDD YSYQGHMQSC NFSAEKAKVY INDSVELSQN EQKLAAWLAK RGPISVAINA FGMQFYRHGI SRPLRPLCSP WLIDHAVLLV GYGNRSDVPF WAIKNSWGTD WGEKGYYYLH RGSGACGVNT MASSAVVD

• Applicable Applications for CTSF recombinant protein: SDS-PAGE, Enzyme Activity
• Endotoxin: <1 EU per 1ug of protein (determined by LAL method)
• Species: Human
• Tag: His-Tag
• Predicted N-term: Ala
• Bioactivity: Specific activity is > 5pmol/min/ug, and is defined as the amount of enzyme that hydrolyze 1pmole of Z-Phe-Arg-AMC to Z-Phe-Arg and AMC per minute at pH5.0 at 37 degree C.
• Preparation and Storage: Can be stored at +2C to +8 degree C for 1 week. For long term storage, aliquot and store at -20C to -80 degree C. Avoid repeated freezing and thawing cycles.

SDS-Page

(3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain)

Related Product Information for CTSF recombinant protein

Cathepsin F, also known as CTSF, belongs to the cathepsin family. Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. This protein is thought to play a role in normal protein catabolism, and because it is highly expressed in some cancer cell lines, it may be involved in degradative processes occurring during tumor progression. The CTSF gene may function as a tumor suppressor in Gastric cancer and may be a potential therapeutic target in the treatment of Gastric cancer. It also can be used as an alternative way to test for the disease known as Opisthorchis Viverrini. Recombinant human Cathepsin F protein, fused to His-tag at C-terminus, was expressed in insect cell and purified by using conventional chromatography techniques.

Product Categories/Family for CTSF recombinant protein

Immunology; Proteins

References

Schmitz J., et al, (2015) ChemMedChem. 10:1365-1377. Ji C., et al, (2018) Oncol Res. 26:83-93. Kulkarni G., et al, (2021) Schizophr Res. 228:435-437.

NCBI and Uniprot Product Information

• NCBI GI #: 6042196
• NCBI GeneID: 8722
• NCBI Accession #: NP_003784
• NCBI GenBank Nucleotide #: NM_003793.3
• UniProt Accession #: Q9UBX1; O95240; Q9NSU4; Q9UKQ5; B2R964
• Molecular Weight: 26 kDa (237aa)
• NCBI Official Full Name: cathepsin F
• NCBI Official Synonym Full Names: cathepsin F
• NCBI Official Symbol: CTSF
• NCBI Official Synonym Symbols: CATSF; CLN13
• NCBI Protein Information: cathepsin F
• UniProt Protein Name: Cathepsin F
• Protein Family: Cathepsin
• UniProt Gene Name: CTSF
• UniProt Synonym Gene Names: CATSF
• UniProt Entry Name: CATF_HUMAN

NCBI Description

Cathepsins are papain family cysteine proteinases that represent a major component of the lysosomal proteolytic system. Cathepsins generally contain a signal sequence, followed by a propeptide and then a catalytically active mature region. The very long (251 amino acid residues) proregion of the cathepsin F precursor contains a C-terminal domain similar to the pro-segment of cathepsin L-like enzymes, a 50-residue flexible linker peptide, and an N-terminal domain predicted to adopt a cystatin-like fold. The cathepsin F proregion is unique within the papain family cysteine proteases in that it contains this additional N-terminal segment predicted to share structural similarities with cysteine protease inhibitors of the cystatin superfamily. This cystatin-like domain contains some of the elements known to be important for inhibitory activity. CTSF encodes a predicted protein of 484 amino acids which contains a 19 residue signal peptide. Cathepsin F contains five potential N-glycosylation sites, and it may be targeted to the endosomal/lysosomal compartment via the mannose 6-phosphate receptor pathway. The cathepsin F gene is ubiquitously expressed, and it maps to chromosome 11q13, close to the gene encoding cathepsin W. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSF: Thiol protease which is believed to participate in intracellular degradation and turnover of proteins. Has also been implicated in tumor invasion and metastasis. Belongs to the peptidase C1 family.

Protein type: Protease; EC 3.4.22.41

Chromosomal Location of Human Ortholog: 11q13

Cellular Component: lysosomal lumen; lysosome; vesicle

Molecular Function: cysteine-type endopeptidase activity

Biological Process: antigen processing and presentation of exogenous peptide antigen via MHC class II; proteolysis

Disease: Ceroid Lipofuscinosis, Neuronal, 13