Chicken Lysosomal protective protein ELISA Kit | CTSA elisa kit

Chicken Lysosomal protective protein ELISA Kit

Cat. Num. AAA745044

• Gene Names: CTSA; GSL; GLB2; NGBE; PPCA; PPGB
• Reactivity: Chicken
• Synonyms: Lysosomal protective protein; Chicken Lysosomal protective protein ELISA Kit; CTSA elisa kit

• Reactivity: Chicken

• Samples: Serum, plasma, Cell Culture Supernatants, body fluid and tissue homogenate
• Assay Type: Competitive
• Sensitivity: 1.0 pg/mL.
• Intended Uses: This CTSA ELISA kit is a 1.5 hour solid-phase ELISA designed for the quantitative determination of Chicken CTSA. This ELISA kit for research use only, not for therapeutic applications
• Preparation and Storage: Store all reagents at 2-8 degree C.

Typical Testing Data/Standard Curve (for reference only)

Related Product Information for CTSA elisa kit

Principle of the Assay: CTSA ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-CTSA antibody and an CTSA-HRP conjugate. The assay sample and buffer are incubated together with CTSA-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the CTSA concentration since CTSA from samples and CTSA-HRP conjugate compete for the anti-CTSA antibody binding site. Since the number of sites is limited, as more sites are occupied by CTSA from the sample, fewer sites are left to bind CTSA-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The CTSA concentration in each sample is interpolated from this standard curve.

NCBI and Uniprot Product Information

• NCBI GI #: 262527235
• NCBI GeneID: 5476
• NCBI Accession #: NP_001161066.1
• NCBI GenBank Nucleotide #: NM_001167594.1
• Molecular Weight: 52,489 Da
• NCBI Official Full Name: lysosomal protective protein isoform c
• NCBI Official Synonym Full Names: cathepsin A
• NCBI Official Symbol: CTSA
• NCBI Official Synonym Symbols: GSL; GLB2; NGBE; PPCA; PPGB
• NCBI Protein Information: lysosomal protective protein; deamidase; urinary kininase; carboxypeptidase C; carboxypeptidase L; carboxypeptidase-L; beta-galactosidase 2; lysosomal carboxypeptidase A; protective protein cathepsin A; carboxypeptidase Y-like kininase; beta-galactosidase protective protein
• UniProt Protein Name: Lysosomal protective protein
• Protein Family: Lysosomal protective protein
• UniProt Gene Name: CTSA
• UniProt Synonym Gene Names: PPGB; PPCA
• UniProt Entry Name: PPGB_HUMAN

NCBI Description

This gene encodes a glycoprotein which associates with lysosomal enzymes beta-galactosidase and neuraminidase to form a complex of high molecular weight multimers. The formation of this complex provides a protective role for stability and activity. Deficiencies in this gene are linked to multiple forms of galactosialidosis. Alternatively spliced transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]

Uniprot Description

CTSA: Protective protein appears to be essential for both the activity of beta-galactosidase and neuraminidase, it associates with these enzymes and exerts a protective function necessary for their stability and activity. This protein is also a carboxypeptidase and can deamidate tachykinins. Defects in CTSA are the cause of galactosialidosis (GSL). A lysosomal storage disease associated with a combined deficiency of beta-galactosidase and neuraminidase, secondary to a defect in cathepsin A. All patients have clinical manifestations typical of a lysosomal disorder, such as coarse facies, cherry red spots, vertebral changes, foam cells in the bone marrow, and vacuolated lymphocytes. Three phenotypic subtypes are recognized. The early infantile form is associated with fetal hydrops, edema, ascites, visceromegaly, skeletal dysplasia, and early death. The late infantile type is characterized by hepatosplenomegaly, growth retardation, cardiac involvement, and a normal or mildly affected mental state. The juvenile/adult form is characterized by myoclonus, ataxia, angiokeratoma, mental retardation, neurologic deterioration, absence of visceromegaly, and long survival. Belongs to the peptidase S10 family.

Protein type: EC 3.4.16.5; Protease; Mitochondrial; Endoplasmic reticulum

Chromosomal Location of Human Ortholog: 20q13.1

Cellular Component: nucleoplasm; lysosomal lumen; membrane; intracellular membrane-bound organelle; endoplasmic reticulum; lysosome

Molecular Function: serine carboxypeptidase activity; carboxypeptidase activity; enzyme activator activity

Biological Process: positive regulation of catalytic activity; intracellular protein transport; sphingolipid metabolic process; cellular protein metabolic process; dolichol-linked oligosaccharide biosynthetic process; glycosphingolipid metabolic process; protein amino acid N-linked glycosylation via asparagine; post-translational protein modification; proteolysis

Disease: Galactosialidosis