Typical Testing Data/Standard Curve (for reference only)
Canine Cardiac Troponin 1 ELISA Kit | cTn1 elisa kit
Canine Cardiac Troponin 1 ELISA Kit
Typical Testing Data/Standard Curve (for reference only)
Typical Testing Data/Standard Curve (for reference only)
Principle of the Assay: cTnI ELISA kit applies the competitive enzyme immunoassay technique utilizing a monoclonal anti-cTnI antibody and an cTnI-HRP conjugate. The assay sample and buffer are incubated together with cTnI-HRP conjugate in pre-coated plate for one hour. After the incubation period, the wells are decanted and washed five times. The wells are then incubated with a substrate for HRP enzyme. The product of the enzyme-substrate reaction forms a blue colored complex. Finally, a stop solution is added to stop the reaction, which will then turn the solution yellow. The intensity of color is measured spectrophotometrically at 450nm in a microplate reader. The intensity of the color is inversely proportional to the cTnI concentration since cTnI from samples and cTnI-HRP conjugate compete for the anti-cTnI antibody binding site. Since the number of sites is limited, as more sites are occupied by cTnI from the sample, fewer sites are left to bind cTnI-HRP conjugate. A standard curve is plotted relating the intensity of the color (O.D.) to the concentration of standards. The cTnI concentration in each sample is interpolated from this standard curve.
NCBI and Uniprot Product Information
NCBI Description
Troponin I (TnI), along with troponin T (TnT) and troponin C (TnC), is one of 3 subunits that form the troponin complex of the thin filaments of striated muscle. TnI is the inhibitory subunit; blocking actin-myosin interactions and thereby mediating striated muscle relaxation. The TnI subfamily contains three genes: TnI-skeletal-fast-twitch, TnI-skeletal-slow-twitch, and TnI-cardiac. This gene encodes the TnI-cardiac protein and is exclusively expressed in cardiac muscle tissues. Mutations in this gene cause familial hypertrophic cardiomyopathy type 7 (CMH7) and familial restrictive cardiomyopathy (RCM). [provided by RefSeq, Jul 2008]
Uniprot Description
TNNI3: Troponin I is the inhibitory subunit of troponin, the thin filament regulatory complex which confers calcium-sensitivity to striated muscle actomyosin ATPase activity. Binds to actin and tropomyosin. Interacts with TRIM63. Interacts with STK4/MST1. Belongs to the troponin I family.
Protein type: Motor; Actin-binding; Motility/polarity/chemotaxis
Chromosomal Location of Human Ortholog: 19q13.4
Cellular Component: sarcomere; troponin complex; cytosol
Molecular Function: troponin T binding; protein domain specific binding; troponin C binding; protein binding; metal ion binding; calcium channel inhibitor activity; actin binding; protein kinase binding; calcium-dependent protein binding
Biological Process: regulation of smooth muscle contraction; cellular calcium ion homeostasis; heart contraction; heart development; regulation of systemic arterial blood pressure by ischemic conditions; ventricular cardiac muscle morphogenesis; vasculogenesis; negative regulation of ATPase activity; cardiac muscle contraction; muscle filament sliding
Disease: Cardiomyopathy, Dilated, 1ff; Cardiomyopathy, Familial Restrictive, 1; Cardiomyopathy, Familial Hypertrophic, 7; Cardiomyopathy, Dilated, 2a
Research Articles on cTn1
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Product Notes
The Canine cTn1 tnni3 (Catalog #AAA754040) is an ELISA Kit and is intended for research purposes only. The product is available for immediate purchase. The AAA754040 ELISA Kit recognizes Canine cTn1. It is sometimes possible for the material contained within the vial of "Cardiac Troponin 1, ELISA Kit" to become dispersed throughout the inside of the vial, particularly around the seal of said vial, during shipment and storage. We always suggest centrifuging these vials to consolidate all of the liquid away from the lid and to the bottom of the vial prior to opening. Please be advised that certain products may require dry ice for shipping and that, if this is the case, an additional dry ice fee may also be required.Precautions
All products in the AAA Biotech catalog are strictly for research-use only, and are absolutely not suitable for use in any sort of medical, therapeutic, prophylactic, in-vivo, or diagnostic capacity. By purchasing a product from AAA Biotech, you are explicitly certifying that said products will be properly tested and used in line with industry standard. AAA Biotech and its authorized distribution partners reserve the right to refuse to fulfill any order if we have any indication that a purchaser may be intending to use a product outside of our accepted criteria.Disclaimer
Though we do strive to guarantee the information represented in this datasheet, AAA Biotech cannot be held responsible for any oversights or imprecisions. AAA Biotech reserves the right to adjust any aspect of this datasheet at any time and without notice. It is the responsibility of the customer to inform AAA Biotech of any product performance issues observed or experienced within 30 days of receipt of said product. To see additional details on this or any of our other policies, please see our Terms & Conditions page.Item has been added to Shopping Cart
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