Mouse anti-Human MRP5 Monoclonal Antibody | anti-MRP5 antibody

MRP5 (Multidrug Resistance-associated Protein 5, ATP-binding Cassette Sub-family C Member 5, Multi-specific Organic Anion Transporter C, MOAT-C, pABC11, SMRP, ABCC5) (HRP)

Cat. Num. AAA6153588

• Gene Names: ABCC5; MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• Reactivity: Human
• Applications: ELISA, Western Blot
• Purity: Purified by Protein A Affinity Chromatography.
• Synonyms: MRP5; Monoclonal Antibody; MRP5 (Multidrug Resistance-associated Protein 5; ATP-binding Cassette Sub-family C Member 5; Multi-specific Organic Anion Transporter C; MOAT-C; pABC11; SMRP; ABCC5) (HRP); anti-MRP5 antibody

• Host: Mouse
• Reactivity: Human
• Clonality: Monoclonal
• Isotype: IgG2a,k
• Clone Number: 1B12
• Specificity: Recognizes human ABCC5.
• Purity/Purification: Purified by Protein A Affinity Chromatography.
• Form/Format: Supplied as a liquid in PBS, pH 7.2. No preservative added. Labeled with horseradish peroxidase (HRP).
• Sequence Length: 5790

• Applicable Applications for anti-MRP5 antibody: ELISA (EIA), Western Blot (WB)
• Application Notes: Applications are based on unconjugated antibody.
• Immunogen: Partial recombinant corresponding to aa760-846 from ABCC5 (NP_005679) with GST tag. MW of the GST tag alone is 26kD.
• Immunogen Sequence: CITERGTHEELMNLNGDYATIFNNLLLGETPPVEINSKKETSGSQKKSQDKGPKTGSVKKEKAVKPEEGQLVQLEEKGQGSVPWSVY
• Conjugate: HRP
• Preparation and Storage: Store product at 4 degree C if to be used immediately within two weeks. For long-term storage, aliquot to avoid repeated freezing and thawing and store at -20 degree C. Aliquots are stable at -20 degree C for 12 months after receipt. Dilute required amount only prior to immediate use. Further dilutions can be made in assay buffer. Note: Sodium azide is a potent inhibitor of peroxidase and should not be added to HRP conjugates. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap.

Western Blot (WB)

(Western Blot detection against Immunogen (35.31kD).)

Testing Data

(Detection limit for recombinant GST tagged ABCC5 is 0.03ng/ml as a capture antibody.)

Related Product Information for anti-MRP5 antibody

ABCC5 is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients.

Product Categories/Family for anti-MRP5 antibody

Antibodies; Multi Drug Resistance (MDR)

NCBI and Uniprot Product Information

• NCBI GI #: 1519243001
• NCBI GeneID: 10057
• NCBI Accession #: NM_005688.4
• NCBI GenBank Nucleotide #: NM_005688
• UniProt Accession #: O15440
• NCBI Official Full Name: Homo sapiens ATP binding cassette subfamily C member 5 (ABCC5), transcript variant 1, mRNA
• NCBI Official Synonym Full Names: ATP binding cassette subfamily C member 5
• NCBI Official Symbol: ABCC5
• NCBI Official Synonym Symbols: MRP5; SMRP; ABC33; MOATC; MOAT-C; pABC11; EST277145
• NCBI Protein Information: multidrug resistance-associated protein 5
• UniProt Protein Name: Multidrug resistance-associated protein 5
• Protein Family: Probable 37S ribosomal protein
• UniProt Gene Name: ABCC5
• UniProt Synonym Gene Names: MRP5; MOAT-C
• UniProt Entry Name: MRP5_HUMAN

NCBI Description

The protein encoded by this gene is a member of the superfamily of ATP-binding cassette (ABC) transporters. ABC proteins transport various molecules across extra- and intra-cellular membranes. ABC genes are divided into seven distinct subfamilies (ABC1, MDR/TAP, MRP, ALD, OABP, GCN20, White). This protein is a member of the MRP subfamily which is involved in multi-drug resistance. This protein functions in the cellular export of its substrate, cyclic nucleotides. This export contributes to the degradation of phosphodiesterases and possibly an elimination pathway for cyclic nucleotides. Studies show that this protein provides resistance to thiopurine anticancer drugs, 6-mercatopurine and thioguanine, and the anti-HIV drug 9-(2-phosphonylmethoxyethyl)adenine. This protein may be involved in resistance to thiopurines in acute lymphoblastic leukemia and antiretroviral nucleoside analogs in HIV-infected patients. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Feb 2016]

Uniprot Description

ABCC5: Acts as a multispecific organic anion pump which can transport nucleotide analogs. Belongs to the ABC transporter superfamily. ABCC family. Conjugate transporter (TC 3.A.1.208) subfamily. 4 isoforms of the human protein are produced by alternative splicing.

Protein type: Membrane protein, integral; Membrane protein, multi-pass; Transporter; Transporter, ABC family

Chromosomal Location of Human Ortholog: 3q27

Cellular Component: membrane; integral to plasma membrane; plasma membrane

Molecular Function: ATPase activity, coupled to transmembrane movement of substances; organic anion transmembrane transporter activity; ATP binding

Biological Process: glycosaminoglycan metabolic process; transport; hyaluronan biosynthetic process; carbohydrate metabolic process; pathogenesis; transmembrane transport; hyaluronan metabolic process; organic anion transport