Cystatin SN / CST1 Recombinant Protein | CST1 recombinant protein

Recombinant Human Cystatin SN / CST1 Protein (His tag)

Cat. Num. AAA2545742

• Purity: > 97 % as determined by SDS-PAGE
• Synonyms: Cystatin SN / CST1; Recombinant Human Cystatin SN / CST1 Protein (His tag); CST1; CST1 recombinant protein

• Host: Human Cells
• Purity/Purification: > 97 % as determined by SDS-PAGE
• Form/Format: Lyophilized from sterile PBS, pH 7.4, 5% Trehalose, 5% mannitol, 0.01% Tween 80
• Sequence Length: 141

• Application Notes: The secreted recombinant human CST1 consists of 132 amino acids and has a calculated molecular mass of 15.6 kDa. It migrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions as predicted.
• Predicted N Terminal: Trp 21
• Source: Human
• Endotoxin: < 1.0 EU per ug of the protein as determined by the LAL method
• Reconstitution: It is recommended that sterile water (130 uL) be added to the vial to prepare a stock solution of 0.15 mg/mL; Concentration is measured by UV-Vis.
• Stability: Samples are stable for up to twelve months from date of receipt at -70°C
• Preparation and Storage: Store it under sterile conditions at -20°C to -80°C. It is recommended that the protein be aliquoted and be used as soon as possible. ; ; Avoid repeated freeze-thaw cycles.

SDS-PAGE

Related Product Information for CST1 recombinant protein

Background: Cystatin-SN, also known as Cystain-SA-I, Cystatin-1, Salivary cystatin-SA-1 and CST1, is a secreted protein which belongs to the cystatin family. The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. Cystatin-SN / CST1 is expressed in submandibular and sublingual saliva but not in parotid saliva (at protein level). Cystatin-SN / CST1 is also expressed in saliva, tears, urine and seminal fluid. Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Human salivary cystatins include Cystatin-S, Cystatin-S1, Cystatin-S2, Cystatin- SA, Cystatin-SN, Cystatin-C and Cystatin-D. Cystatin-SN is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin-S, although both inhibit ficin equally well.

Description: A DNA sequence encoding the human CST1 (P01037-1) (Met 1-Ser 141) was fused with a polyhistidine tag at the C-terminus.

NCBI and Uniprot Product Information

• NCBI GI #: 19882251
• NCBI GeneID: 1469
• NCBI Accession #: NP_001889.2
• NCBI GenBank Nucleotide #: NM_001898.2
• UniProt Accession #: P01037; Q96LE6; Q9UCQ6
• Molecular Weight: The secreted recombinant human CST1 consists of 132 amino acids and has a calculated molecular mass of 15.6 kDa. It migrates as an approximately 17 kDa band in SDS-PAGE under reducing conditions as predicted.
• NCBI Official Full Name: cystatin-SN
• NCBI Official Synonym Full Names: cystatin SN
• NCBI Official Symbol: CST1
• NCBI Protein Information: cystatin-SN
• UniProt Protein Name: Cystatin-SN
• Protein Family: Cystatin
• UniProt Gene Name: CST1

NCBI Description

The cystatin superfamily encompasses proteins that contain multiple cystatin-like sequences. Some of the members are active cysteine protease inhibitors, while others have lost or perhaps never acquired this inhibitory activity. There are three inhibitory families in the superfamily, including the type 1 cystatins (stefins), type 2 cystatins and the kininogens. The type 2 cystatin proteins are a class of cysteine proteinase inhibitors found in a variety of human fluids and secretions, where they appear to provide protective functions. The cystatin locus on chromosome 20 contains the majority of the type 2 cystatin genes and pseudogenes. This gene is located in the cystatin locus and encodes a cysteine proteinase inhibitor found in saliva, tears, urine, and seminal fluid. [provided by RefSeq, Jul 2008]

Uniprot Description

CST1: Human saliva appears to contain several cysteine proteinase inhibitors that are immunologically related to cystatin S but that differ in their specificity due to amino acid sequence differences. Cystatin SN, with a pI of 7.5, is a much better inhibitor of papain and dipeptidyl peptidase I than is cystatin S, although both inhibit ficin equally well. Belongs to the cystatin family.

Protein type: Secreted; Secreted, signal peptide

Chromosomal Location of Human Ortholog: 20p11.21

Cellular Component: extracellular space

Molecular Function: protease binding; protein binding

Biological Process: detection of chemical stimulus involved in sensory perception of bitter taste