Mouse G-CSF ELISA Kit | CSF3 elisa kit

Mouse G-CSF PicoKine ELISA Kit

Cat. Num. AAA175979

• Gene Names: Csf3; Csfg; G-CSF; MGI-IG
• Reactivity: Mouse
• Synonyms: G-CSF; Mouse G-CSF PicoKine ELISA Kit; Granulocyte colony-stimulating factor; colony stimulating factor 3 (granulocyte); C17orf33; Colony stimulating factor 3 (granulocyte); CSF 3; CSF beta; CSF3; CSF3OS; Csfg; Filgrastim; GCSA; GCSF; Granulocyte colony stimulating fact; Lenograstim; Macrophage granulocyte inducer 2; MGC45931; MGI 2; MGI IG; OTTMUSP00000006785; Pluripoietin; RP23-395E10.4; CSF3 elisa kit

• Reactivity: Mouse
• Specificity: Natural and recombinant mouse G-CSF
• Sequence Length: 208

• Samples: Cell culture supernates, serum and plasma (heparin, EDTA)
• Assay Type: Sandwich
• Detection Range: 31.2pg/ml-2000pg/ml
• Sensitivity: < 4pg/ml
• Preparation and Storage: Store at 4 degree C for 6 months, at -20 degree C for 12 months. Avoid multiple freeze-thaw cycles (Shipped with wet ice.)

Typical Testing Data/Standard Curve (for reference only)

(Mouse G-CSF PicoKine ELISA Kit standard curve )

Related Product Information for CSF3 elisa kit

Principle of the assay: mouse G-CSF ELISA Kit was based on standard sandwich enzyme-linked immune-sorbent assay technology. A monoclonal antibody from rat specific for G-CSF has been precoated onto 96-well plates. Standards (E.coli,V31-A208) and test samples are added to the wells, a biotinylated detection polyclonal antibody from goat specific for G-CSF is added subsequently and then followed by washing with PBS or TBS buffer. Avidin-Biotin-Peroxidase Complex was added and unbound conjugates were washed away with PBS or TBS buffer. HRP substrate TMB was used to visualize HRP enzymatic reaction. TMB was catalyzed by HRP to produce a blue color product that changed into yellow after adding acidic stop solution. The density of yellow is proportional to the mouse G-CSF amount of sample captured in plate.
Background: Granulocyte colony-stimulating factor (G-CSF) is a member of the CSF family of hormone-like glycoprotein that regulates hematopoietic cell proliferation and differentiation, and it almost exclusively stimulates the colony formation of granulocytes from committed precursor cells in semi-solid agar culture.1 G-CSF is also termed colonystimulating factor-3, and a single gene of which codes for a 177 or 180 amino acid mature protein of molecular weight 19,600. Functionally, it specifically stimulates the proliferation and differentiation of the progenitor cells for granulocytes. The effect of G-CSF on myeloid leukemias is unique among colony stimulating factors in driving the leukemic cells from a self-renewing malignant state to a mature differentiated phenotype with the concomitant loss of tumorigenicity.2 Besides, it also prevents cardiac remodeling after myocardial infarction by activating the Jak-Statpathway in cardiomyocytes. The recombinant form of hG-CSF is capable of supporting neutrophil proliferation in a CFU-GM assay as well as early erythroid colonies and mixed colony formation. Human gene coding for G-CSF is assigned to the q21-q22 region of chromosome 17.3 The standard product used in this kit is recombinant mouseG-CSF, consisting of 179 amino acids with the molecular mass of 19KDa.

NCBI and Uniprot Product Information

• NCBI GI #: 117565
• NCBI GeneID: 12985
• UniProt Accession #: P09920
• Molecular Weight: 22,421 Da
• NCBI Official Full Name: Granulocyte colony-stimulating factor
• NCBI Official Synonym Full Names: colony stimulating factor 3 (granulocyte)
• NCBI Official Symbol: Csf3
• NCBI Official Synonym Symbols: Csfg; G-CSF; MGI-IG
• NCBI Protein Information: granulocyte colony-stimulating factor
• UniProt Protein Name: Granulocyte colony-stimulating factor
• Protein Family: Granulocyte colony-stimulating factor
• UniProt Gene Name: Csf3
• UniProt Synonym Gene Names: Csfg; G-CSF
• UniProt Entry Name: CSF3_MOUSE

Uniprot Description

G-CSF: Granulocyte/macrophage colony-stimulating factors are cytokines that act in hematopoiesis by controlling the production, differentiation, and function of 2 related white cell populations of the blood, the granulocytes and the monocytes-macrophages. This CSF induces granulocytes. Belongs to the IL-6 superfamily. 2 isoforms of the human protein are produced by alternative splicing.

Protein type: Cell cycle regulation; Secreted; Cytokine; Secreted, signal peptide

Cellular Component: extracellular space; extracellular region

Molecular Function: enzyme binding; growth factor activity; cytokine activity

Biological Process: positive regulation of phosphoinositide 3-kinase cascade; positive regulation of protein kinase B signaling cascade; positive regulation of peptidyl-tyrosine phosphorylation; positive regulation of myeloid cell differentiation; positive regulation of actin filament polymerization; positive regulation of protein binding; granulocyte differentiation; positive regulation of cell proliferation; positive regulation of transcription factor import into nucleus; positive regulation of transcription from RNA polymerase II promoter; immune response; positive regulation of peptidyl-serine phosphorylation