Granulocyte Macrophage-Colony Stimulating Factor Recombinant Protein | GM-CSF recombinant protein

Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant

Cat. Num. AAA553079

• Purity: >96%, as determined by SDS-PAGE and HPLC
• Synonyms: Granulocyte Macrophage-Colony Stimulating Factor; Human Granulocyte Macrophage-Colony Stimulating Factor Recombinant; GM-CSF; gmcsf; csf2; GM-CSF recombinant protein

• Host: E Coli
• Purity/Purification: >96%, as determined by SDS-PAGE and HPLC
• Form/Format: Human GM-CSF was lyophilized from a 0.2 um filtered solution in PBS, pH 7.0.
• Sequence: MWLQSLLLLG TVACSISAPA RSPSPSTQPW EHVNAIQEAR RLLNLSRDTA AEMNETVEVI SEMFDLQEPT CLQTRLELYK QGLRGSLTKL KGPLTMMASH YKQHCPPTPE TSCATQIITF ESFKENLKDF LLVIPFDCWE PVQE
• Sequence Length: 144

• Domain: four helix cytokine core
• Host Note: Optimized DNA sequence encoding Human Granulocyte Macrophage-Colony Stimulating Factor mature chain was expressed in Escherichia Coli.
• Endotoxin: Endotoxin content was assayed using a LAL gel clot method.; Endotoxin level was found to be less than 0.1 ng/ug (1EU/ug).
• Reconstitution: A quick spin of the vial followed by reconstitution in distilled water to a concentration not less than 0.1 mg/mL. This solution can then be diluted into other buffers.
• Molecular Weight Note: Native human Granulocyte Macrophage-Colony Stimulating Factor is generated by the proteolytic removal of the signal peptide and propeptide,this molecule has a calculated mass of 15 kDa.; ; Recombinant GM-CSF is a disulfide-linked monomer protein, consisting of 128 amino acids and migrates as an approximately 15 kDa protein under reducing conditions.
• Preparation and Storage: The lyophilized protein is stable for at least 2 years from date of receipt at -20 degree C.; Upon reconstitution, this cytokine can be stored in working aliquots at 2 degree - 8 degree C for one month, or at -20 degree C for six months, with a carrier protein without detectable loss of activity. ; ; Avoid repeated freeze/thaw cycles.

Related Product Information for GM-CSF recombinant protein

GM-CSF is a monomeric protein of 127 amino acids with two glycosylation sites. The protein is synthesized as a precursor of 144 amino acids, which included a hydrophobic secretory signal sequence at the aminoterminal end. The sugar moiety is not required for the full spectrum of biological activities. Non- glycosylated and glycosylated GM-CSF show the same activities in vitro. Fully glycosylated GM-CSF is biologically more active in vivo than the non- glycosylated protein. This protein is secreted together with other factors by T-cells and macrophages following cell activation by antigens or mitogens. Approximately 90% of the secreted colony stimulating activities are due to GM- CSF.

Product Categories/Family for GM-CSF recombinant protein

Cytokines

References

• GM-CSF regulates intimal cell proliferation in nascent atherosclerotic lesions .J. Exp. Med., Sep 2009; 206: 2141 - 2149.
• the transcription factors stat5a/b regulate GM-CSF-mediated granulopoiesis .Blood, Sep 2009; 10.1182/blood-2009-04-216390.
• GM-CSF and il-4 induce dendritic cell differentiation and disrupt osteoclastogenesis through m-csf receptor shedding by up-regulation of tnf- converting enzyme (tace) .Blood, Sep 2009; 10.1182/blood-2009-04-215020.
• biologic activity of irradiated, autologous, GM-CSF-secreting leukemia cell vaccines early after allogeneic stem cell transplantation .PNAS, Sep 2009; 106: 15825 - 15830.
• stress response genes are suppressed in mouse preimplantation embryos by granulocyte-macrophage colony-stimulating factor (GM-CSF) .Hum. Reprod., Sep 2009; 10.1093/humrep/dep307.